Biology, vulnerabilities and clinical applications of circulating tumour cells

Ring, Alexander; Nguyen-Sträuli, Bich Doan; Wicki, Andreas; Aceto, Nicola

doi:10.1038/s41568-022-00536-4

Cited by 103 publications

(78 citation statements)

References 249 publications

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“…Tumor metastasis is the primary reason for more than 90% of cancer-associated deaths. − In the process of tumor metastasis, circulating tumor cells (CTCs) are shed from the solid tumors and enter the bloodstream, eventually colonizing in distant tissues or organs and forming new metastatic foci. − CTCs have been regarded as important noninvasive biomarkers and hold great potential for early cancer diagnosis, prediction of recurrence, and individualized treatment. − However, the extremely rare CTCs are present in the complex whole blood, which contains only a few to a dozen CTCs per mL while billions of blood cells. − Some materials have been developed to improve the capture performance toward CTCs, while most of them cannot achieve fully stereo contacts with target cells. − Compared with the smooth plane, the three-dimensional (3D) micro-/nanostructure with the ability to interact with cells can significantly improve the capture efficiency of CTCs . In addition, the fragility of CTCs is another key issue that needs to be considered.…”

Section: Introductionmentioning

confidence: 99%

Tumor Microenvironment-Inspired Glutathione-Responsive Three-Dimensional Fibrous Network for Efficient Trapping and Gentle Release of Circulating Tumor Cells

Luo,

He,

et al. 2023

ACS Appl. Mater. Interfaces

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Detection of circulating tumor cells (CTCs) is important for early cancer diagnosis, prediction of postoperative recurrence, and individualized treatment. However, it is still challenging to achieve efficient capture and gentle release of CTCs from the complex peripheral blood due to their rarity and fragility. Herein, inspired by the three-dimensional (3D) network structure and high glutathione (GSH) level of the tumor microenvironment (TME), a 3D stereo (3D-G@FTP) fibrous network is developed by combining the liquid-assisted electrospinning method, gas foaming technique, and metal–polyphenol coordination interactions to achieve efficient trapping and gentle release of CTCs. Compared with the traditional 2D@FTP fibrous scaffold, the 3D-G@FTP fibrous network could achieve higher capture efficiency (90.4% vs 78.5%) toward cancer cells in a shorter time (30 min vs 90 min). This platform showed superior capture performance toward heterogeneous cancer cells (HepG2, HCT116, HeLa, and A549) in an epithelial cell adhesion molecule (EpCAM)-independent manner. In addition, the captured cells with high cell viability (>90.0%) could be gently released under biologically friendly GSH stimulus. More importantly, the 3D-G@FTP fibrous network could sensitively detect 4–19 CTCs from six kinds of cancer patients’ blood samples. We expect this TME-inspired 3D stereo fibrous network integrating efficient trapping, broad-spectrum recognition, and gentle release will promote the development of biomimetic devices for rare cell analysis.

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Section: Introductionmentioning

confidence: 99%

Tumor Microenvironment-Inspired Glutathione-Responsive Three-Dimensional Fibrous Network for Efficient Trapping and Gentle Release of Circulating Tumor Cells

Luo,

He,

et al. 2023

ACS Appl. Mater. Interfaces

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“…After entry into the bloodstream, CTCs (known as inherent metastatic factors) can infiltrate healthy tissues that are distant from the tumour [11]. Nevertheless, CTC clusters display metastatic potential of 20-to 100-fold greater metastatic potential than single CTCs [12][13][14]. Other possible targets of migrastatic agents are mechanisms of cell mobility (e.g., cytoskeletal dynamics and cell contractility) and energy provisions (e.g., ATP availability, mitochondrial metabolism) [15].…”

Section: Introductionmentioning

confidence: 99%

Therapeutic potential and limitations of curcumin as antimetastatic agent

Dytrych¹,

Kejík²,

Hajduch³

et al. 2023

Biomedicine & Pharmacotherapy

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“…Moreover, many details remain unclear as it is almost impossible to predict when and where metastases emerge in vivo. Thus we are often limited to study metastasis via circulating tumor cells, biopsies, and postmortem analysis of metastases 17,18 .…”

Section: Introductionmentioning

confidence: 99%

Direct visualization of emergent metastatic features within anex vivomodel of the tumor microenvironment

Anandi

Garcia

Janská

et al. 2023

Preprint

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Preventing tumor cells from acquiring metastatic properties would significantly reduce cancer mortality. However, due to the complex nature of this process, it remains one of the most poorly understood and untreatable aspects of cancer. Ischemia and hypoxia in solid tumors are requisite in metastasis formation -- conditions that arise far from functional blood vessels and deep within tumor tissues. These secluded locations impede the observation of pre-metastatic tumor cells and their interactions with stromal cells, which are also critical in the initiation of this process. Thus, the initiation of metastasis has been incredibly difficult to model in the lab and to observe in vivo. We present an ex vivo model of the tumor microenvironment, called 3MIC, which overcomes these experimental challenges and enables the observation of ischemic tumor cells in their native 3D context with high spatial and temporal resolutions. The 3MIC recreates ischemic conditions in the tumor microenvironment and facilitates the co-culture of different cell types. Using live microscopy, we showed that ischemia, but not hypoxia alone, increases the motility and invasive properties of cells derived from primary tumors. These changes are phenotypic and can occur without clonal selection. We directly observed how interactions with stromal cells such as macrophages increased tumor invasion in conjunction with the effects of an ischemic microenvironment. Finally, we tested the effects of chemotherapy drugs under different metabolic microenvironments and found that ischemic tumor cells are more resistant to paclitaxel, possibly due to a metabolic resistance mechanism. Overall, the 3MIC is a cost-effective system that allows for the dissection of the complexity of the tumor microenvironment and direct observation of the emergence of metastasis, as well as the testing of treatments that may halt this process.

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Biology, vulnerabilities and clinical applications of circulating tumour cells

Cited by 103 publications

References 249 publications

Tumor Microenvironment-Inspired Glutathione-Responsive Three-Dimensional Fibrous Network for Efficient Trapping and Gentle Release of Circulating Tumor Cells

Tumor Microenvironment-Inspired Glutathione-Responsive Three-Dimensional Fibrous Network for Efficient Trapping and Gentle Release of Circulating Tumor Cells

Therapeutic potential and limitations of curcumin as antimetastatic agent

Direct visualization of emergent metastatic features within anex vivomodel of the tumor microenvironment

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