Biology of the <i>Caenorhabditis elegans</i> Germline Stem Cell System

Hubbard, E. Jane Albert; Schedl, Tim

doi:10.1534/genetics.119.300238

Cited by 100 publications

(56 citation statements)

References 315 publications

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“…Peak LAG-1 accumulation is also repressed by the combined activities of GLD-1 and GLD-2 (Fig 3; S3 Fig). This modest repression is presumably because of lower gld-1 and gld-2 activity in the distal most 10-cell diameters [4]. and numbers in bracket shows the sample size.…”

Section: Plos Geneticsmentioning

confidence: 99%

“…Here we focus on the C. elegans germline stem cell system, which shares a number of features with other stem cell systems [4]. Niche-germline stem cell signaling employs the Notch pathway, which has been extensively studied in C. elegans [5].…”

Section: Introductionmentioning

confidence: 99%

“…The germline is capped by the distal tip cell (DTC), which is a large somatic niche cell that polarizes germline cellular organization. Germ cells adjacent to the DTC are in a region called the progenitor zone, which distally contains stem cells, then cells completing a terminal mitotic cell cycle and cells undergoing meiotic S-phase, followed proximally by cells undergoing the earliest stages of meiotic prophase, leptotene and zygotene [4,6,7] (Fig 1). The DTC expresses two Notch pathway DSL (for Delta, Serate, LAG-2) ligands, LAG-2 and APX-1 [8][9][10].…”

Section: Introductionmentioning

confidence: 99%

“…Second, epistasis analysis place lst-1 and sygl-1, like glp-1, upstream of the meiotic entry pathway genes. Third, lst-1 and sygl-1 nascent transcripts are restricted to the distal most germ cells, contacting the [4]. GLP-1 signaling maintains germline stem cell fate and GLP-1 mediated transcription occurs in the distal most~5 cell diameters (cd) of the germline [21,42].…”

Section: Introductionmentioning

confidence: 99%

“…(B) Genetic pathway controlling the stem cell fate vs meiotic development decision in the distal end of C. elegans germline. GLP-1 signaling acts, at least in part, through transcriptional targets lst-1 and sygl-1 to repress the GLD-1, GLD-2 and SCF PROM-1 meiotic entry pathways (reviewed in [4]). GLP-1(ICD), GLP-1 intracellular domain.…”

Section: Introductionmentioning

confidence: 99%

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GLP-1 Notch—LAG-1 CSL control of the germline stem cell fate is mediated by transcriptional targets lst-1 and sygl-1

et al. 2020

Self Cite

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Stem cell systems are essential for the development and maintenance of polarized tissues. Intercellular signaling pathways control stem cell systems, where niche cells signal stem cells to maintain the stem cell fate/self-renewal and inhibit differentiation. In the C. elegans germline, GLP-1 Notch signaling specifies the stem cell fate, employing the sequence-specific DNA binding protein LAG-1 to implement the transcriptional response. We undertook a comprehensive genome-wide approach to identify transcriptional targets of GLP-1 signaling. We expected primary response target genes to be evident at the intersection of genes identified as directly bound by LAG-1, from ChIP-seq experiments, with genes identified as requiring GLP-1 signaling for RNA accumulation, from RNA-seq analysis. Furthermore, we performed a time-course transcriptomics analysis following auxin inducible degradation of LAG-1 to distinguish between genes whose RNA level was a primary or secondary response of GLP-1 signaling. Surprisingly, only lst-1 and sygl-1, the two known target genes of GLP-1 in the germline, fulfilled these criteria, indicating that these two genes are the primary response targets of GLP-1 Notch and may be the sole germline GLP-1 signaling protein-coding transcriptional targets for mediating the stem cell fate. In addition, three secondary response genes were identified based on their timing following loss of LAG-1, their lack of a LAG-1 ChIP-seq peak and that their glp-1 dependent mRNA accumulation could be explained by a requirement for lst-1 and sygl-1 activity. Moreover, our analysis also suggests that the function of the primary response genes lst-1 and sygl-1 can account for the glp-1 dependent peak protein accumulation of FBF-2, which promotes the stem cell fate and, in part, for the spatial restriction of elevated LAG-1 accumulation to the stem cell region.

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Section: Plos Geneticsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

GLP-1 Notch—LAG-1 CSL control of the germline stem cell fate is mediated by transcriptional targets lst-1 and sygl-1

et al. 2020

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Caenorhabditis elegans septins contribute to the development and structure of the oogenic germline

Perry,

Werner,

Rivenbark

et al. 2023

Cytoskeleton

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Oocytes must be exceptionally large cells in order to support embryonic development. Throughout animal phylogeny, a specialized cell called a syncytium, wherein many nuclei share a continuous cytoplasm, achieves oogenesis. The syncytial nature of germline architecture is key to its function and depends on conserved components of the cortical cytoskeleton. Septins form non‐polar cytoskeletal polymers that associate with membranes. In the syncytial germline of the nematode Caenorhabditis elegans, septins are highly enriched on the cortex and generally required for fertility, but the role of septins in the germline is poorly understood. We report that the C. elegans septins, UNC‐59 and UNC‐61, are important for germline extension during development, the maintenance of its syncytial architecture, and production of oocytes. While much of our findings substantiate the idea that the two C. elegans septins act together, we also found evidence that they have distinct functions. Loss of UNC‐61 perturbed germline extension during germline development, while the loss of UNC‐59 function severely affected germline architecture in adult hermaphrodites. Consultation of clustering results from a large‐scale high‐throughput screen suggested that septins are involved in germ cell proliferation and/or differentiation. In sum, our findings implicate a conserved cytoskeletal component in the complex architecture of a germline syncytium.

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Simplified Quantification of Progenitor Zone Size, an Indicator of Germ Stem Cell Niche Activity, in the Nematode Caenorhabditis elegans

Fausett,

Laury,

Magallon

et al. 2024

Methods in Molecular Biology

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Biology of the Caenorhabditis elegans Germline Stem Cell System

Cited by 100 publications

References 315 publications

GLP-1 Notch—LAG-1 CSL control of the germline stem cell fate is mediated by transcriptional targets lst-1 and sygl-1

GLP-1 Notch—LAG-1 CSL control of the germline stem cell fate is mediated by transcriptional targets lst-1 and sygl-1

Caenorhabditis elegans septins contribute to the development and structure of the oogenic germline

Simplified Quantification of Progenitor Zone Size, an Indicator of Germ Stem Cell Niche Activity, in the Nematode Caenorhabditis elegans

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