Biology of rust-red flour beetle,<i>Tribolium castaneum</i>(Herbst) (Tenebrionidae:Coleoptera)

Devi, M. Bhubaneshwari; Devi, Nirmala

doi:10.5958/0974-8172.2015.00015.2

Cited by 19 publications

(26 citation statements)

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“…Indeed, other workers have found similar results with whole wheat, corn flour, and bran and wheat flakes (Good 1936;Halliday & Blouin-Demers 2014;Devi & Devi 2015). We identified 8 instars in our rearing studies, which is within the range reported for this species (instar range 5-11 with 7-8 most common) (Good 1936;Devi & Devi 2015). Pupal stage duration was similar to previous reports for this species with other food sources at the same temperature range.…”

Section: Discussionsupporting

confidence: 85%

“…We found that the length of the pre-oviposition period in T. castaneum was in agreement with other studies, where T. castaneum females usually initiated oviposition about one wk after mating, and that the period from copulation to oviposition was, on average, four d (Good 1936). Also, the incubation period of T. castaneum eggs was similar to that found by Good (1936), Abdelsamad et al (1988), and Devi and Devi (2015). However, the oviposition period of our beetles, and the number of eggs produced per female fed Brazil nuts, was considerably reduced compared with the same species reared on other diets (at the same temperature) where oviposition continued from 5 to 6 mo (Good 1936;Li & Arbogast 1991).…”

Section: Discussionsupporting

confidence: 83%

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Biological and Reproductive Parameters of Tribolium castaneum in Brazil Nut

Pires

Nogueira

Faroni

et al. 2019

Florida Entomologist

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Section: Discussionsupporting

confidence: 85%

Section: Discussionsupporting

confidence: 83%

Biological and Reproductive Parameters of Tribolium castaneum in Brazil Nut

Pires

Nogueira

Faroni

et al. 2019

Florida Entomologist

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“…We found that TcGr20 expression levels after eclosion in TcGr20 -dsRNA-injected pupae showed a negligible difference compared to those injected with Eluc -dsRNA (S4 Fig). The 6–9-day period of pupal development prior to adulthood [33] may have decreased the gene silencing effect. To test this possibility, we attempted dsRNA injection into starved adult beetles.…”

Section: Discussionmentioning

confidence: 99%

A mannitol/sorbitol receptor stimulates dietary intake in Tribolium castaneum

2017

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In insects, perception of chemical stimuli is involved in the acceptance or rejection of food. Gustatory receptors (Grs) that regulate external signals in chemosensory organs have been found in many insects. Tribolium castaneum, a major pest of stored products, possesses over 200 Gr genes. An expanded repertoire of Gr genes appears to be required for diet recognition in species that are generalist feeders; however, it remains unclear whether T. castaneum recognizes a suite of chemicals common to many products or whether its feeding is activated by specific chemicals, and whether its Grs are involved in feeding behavior. It is difficult to determine the food preferences of T. castaneum based on dietary intake due to a lack of appropriate methodology. This study established a novel dietary intake estimation method using gypsum, designated the TribUTE (Tribolium Urges To Eat) assay. For this assay, T. castaneum adults were fed a gypsum block without added organic compounds. Sweet preference was determined by adding sweeteners and measuring the amount of gypsum in the excreta. Mannitol was the strongest activator of T. castaneum dietary intake. In a Xenopus oocyte expression, TcGr20 was found to be responsible for mannitol and sorbitol responses, but not for responses to other tested non-volatile compounds. The EC50 values of TcGr20 for mannitol and sorbitol were 72.6 mM and 90.6 mM, respectively, suggesting that TcGr20 is a feasible receptor for the recognition of mannitol at lower concentrations. We used RNAi and the TribUTE assay to examine whether TcGr20 expression was involved in mannitol recognition. The amounts of excreta in TcGr20 dsRNA-injected adults decreased significantly, despite the presence of mannitol, compared to control adults. Taken together, our results indicate that T. castaneum adults recognized mannitol/sorbitol using the TcGr20 receptor, thereby facilitating the dietary intake of these compounds.

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“…To explore effective RNAi in T. castaneum, TcGr20-dsRNA was injected into pupae; however, the TcGr20 expression levels in TcGr20 -dsRNA-injected adult beetles after eclosion showed a negligible difference compared to those injected with Eluc -dsRNA injection (S3 Fig). The 6-9-day period of pupal development prior to adulthood [29] may have decreased the gene silencing effect To test this possibility, we attempted dsRNA injection into starved adult beetles. The test showed a significant RNAi silencing effect (Fig 7A).…”

Section: Discussionmentioning

confidence: 99%

A mannitol/sorbitol receptor stimulates dietary intake inTribolium castaneum

Takada

Satō

Kikuta

2017

Preprint

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Perception of chemical stimuli by insects aids in accepting or rejecting food. Gustatory receptors (Grs) regulating external signals in chemosensory organs have been found in many insects. Tribolium castaneum, a major pest of stored products, possesses over 200 Gr genes. An expanded repertoire of Gr genes appears to be required for diet recognition in generalist feeders; however, it remains unclear whether T. castaneum recognizes a suite of chemicals common to many products or whether it is attracted to specific chemicals, and whether its Grs are involved in its feeding behavior. It is difficult to determine the food preference of T. castaneum based on its dietary intake due to a lack of appropriate methodology. This study established a novel dietary intake estimation method using gypsum, designated the TribUTE (Tribolium Urges To Eat) assay. T. castaneum adults were fed gypsum block without added organic compounds. Sugar preference was determined by adding sweeteners and measuring the amount of gypsum in the excreta. Mannitol was the strongest attractant of T. castaneum dietary intake; in addition, TcGr20 was responsible for mannitol and sorbitol responses in Xenopus oocyte expression, but did not respond to any other non-volatile compounds tested. The EC50 values of TcGr20 for mannitol and sorbitol were 72.6 mM and 90.6 mM, respectively, suggesting that TcGr20 is a feasible receptor for the recognition of mannitol in lower concentrations. TcGr20 was expressed in the antennae, where the perception of mannitol would occur. We examined whether TcGr20 was involved in mannitol recognition using RNAi and the TribUTE assay. The amounts of excreta in TcGr20 dsRNA-injected adults decreased significantly despite the presence of mannitol, compared to that of the control adults. Taken together, our results suggest that T. castaneum adults recognized mannitol/sorbitol using TcGr20 receptors, thereby facilitating their dietary intake.AbbreviationORFopen reading frameRTreverse transcriptionRNAiRNA interferencedsRNAdouble strand RNAcRNAcapped RNAMBSmodified Barth’s salineCBBCoomassie brilliant blueGrgustatory receptor

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Biology of rust-red flour beetle,Tribolium castaneum(Herbst) (Tenebrionidae:Coleoptera)

Cited by 19 publications

References 0 publications

Biological and Reproductive Parameters of Tribolium castaneum in Brazil Nut

Biological and Reproductive Parameters of Tribolium castaneum in Brazil Nut

A mannitol/sorbitol receptor stimulates dietary intake in Tribolium castaneum

A mannitol/sorbitol receptor stimulates dietary intake inTribolium castaneum

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