Biology of Floral Scent Volatiles in Ornamental Plants

Ghissing, Upashana; Mitra, Adinpunya

doi:10.1007/978-981-15-1554-5_27-1

Cited by 4 publications

(5 citation statements)

References 47 publications

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“…The MEP pathway is vital for biosynthesis of monoterpenes in plants (Nagegowda et al . 2010; Ghissing & Mitra 2021). Another 14 unigenes encoding eight enzymes were annotated to the mevalonate (MVA) pathway that synthesizes sesquiterpenes (Table S7).…”

Section: Resultsmentioning

confidence: 99%

Comparative transcriptome analysis reveals an insight into the candidate genes involved in anthocyanin and scent volatiles biosynthesis in colour changing flowers of Combretum indicum

et al. 2022

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Combretum indicum is a widely cultivated ornamental species displaying the distinct phenomenon of floral colour change. Flowers display a gradual colour change from white to red, attributed to increased cyanidin 3-O glucoside in petal tissues. The differently coloured flowers also emanate a complex blend of VOCs with trans-linalool oxide (furanoid) as the major compound in the emission profile.• To understand molecular mechanisms regulating floral colour shifts and scent biosynthesis, we performed Illumina transcriptome sequencing, including de novo assembly and functional annotation, for the two stages of floral maturation (white and red). Homology analysis with functional classification identified 84 and 42 candidate genes associated with pigment and scent biosynthesis, respectively. Genes encoding transcription factors, such as MYB, ERF, WD40, WRKY, NAC, bHLH and bZIP, that play critical roles in regulating specialized metabolism were also identified in the transcriptome data.• Differences in expression of genes were consistent with accumulation patterns of anthocyanins in the two different flower colours. A clear upregulation of flavonoid biosynthesis genes in red flower tissue is associated with increased pigment content. RT-qPCR-based expression analyses gave results consistent with the RNA-Seq data, suggesting the sequencing data are consistent and reliable.• This study presents the first report of genetic information for C. indicum. Gene sequences generated from RNA-Seq, along with candidate genes identified by pathway mapping and their expression profiles, provide a valuable resource for subsequent studies towards molecular understanding of specialized metabolism in C. indicum flowers.

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Section: Resultsmentioning

confidence: 99%

Comparative transcriptome analysis reveals an insight into the candidate genes involved in anthocyanin and scent volatiles biosynthesis in colour changing flowers of Combretum indicum

et al. 2022

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“…Volatile production is usually linked with certain cells or compartments like secretory cells or resin ducts (Ghissing & Mitra, 2020). However, these specialized tissue-scale cavities were not observed in E. oxypetalum petal.…”

Section: Discussionmentioning

confidence: 99%

“…The copyright holder for this this version posted November 27, 2023. ; https://doi.org/10. 1101/2023.11.26.568706 doi: bioRxiv preprint resin ducts (Ghissing & Mitra, 2020). However, these specialized tissue-scale cavities were not observed in E. oxypetalum petal.…”

Section: Subcellular Structure Might Participate In the Explosive Sce...mentioning

confidence: 98%

Floral scent emission ofEpiphyllum oxypetalum: identification of a novel cytosol-localized geraniol biosynthesis pathway

Zhang,

Tian

et al. 2023

Preprint

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SummaryEpiphyllum oxypetalum, a renowned ornamental species in Cactaceae, unveils its attractive fragrance during its infrequent and rapid night blooming. However, the nature of this floral scent remains unexplored.Employing GC-MS, transcriptome and biochemical assay, we systematically characterized the composition, emission dynamics and biosynthesis ofE. oxypetalumfloral scent.Composition of the floral scent was highly dynamic, with 72.54% trans-Geraniol, 12.96% benzyl alcohol and 3.75% methyl salicylate at full bloom. From 22:00 to 05:00 the following day, the strong scent was primarily released from petals and sepals. Proved by inhibitor assay, the precursors for the dominant scent volatile trans-Geraniol are derived from amyloplast degradation and mevalonate (MVA) pathway. Together with the cytosol-localized geraniol pyrophosphate synthaseEoGDPSand geraniol synthaseEoTPSa1, we proved an uncanonical cytoplasmic biosynthesis route for monoterpene volatiles inE. oxypetalumpetal.Our study on the dynamic floral scent profile and its biosynthesis posed the first detailed analysis of cactus floral scent. These results laid foundation for novel perfume development and future inquiries on specialized pollination coevolution in Cactaceae.

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“…Floral scents (FSs), comprising a diverse range of low molecular weight (30–300 amu) volatile compounds [ 1 , 2 ], are often heralded as the quintessence of flowers, embodying one of the three fundamental elements alongside shape and color. They serve as a critical criterion for assessing cut flowers [ 3 ].…”

Section: Introductionmentioning

confidence: 99%

Physiological and Biochemical Analysis Revealing the Key Factors Influencing 2-Phenylethanol and Benzyl Alcohol Production in Crabapple Flowers

Peng,

Tao,

et al. 2024

Plants

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Floral scent (FS) plays a crucial role in the ecological functions and industrial applications of plants. However, the physiological and metabolic mechanisms underlying FS formation remain inadequately explored. Our investigation focused on elucidating the differential formation mechanisms of 2-phenylethanol (2-PE) and benzyl alcohol (BA) by examining seven related enzyme concentrations and the content of soluble sugar, soluble proteins, carbon (C) and nitrogen (N), as well as the C/N ratio. The findings revealed that the peak content of 2-PE in M. ‘Praire Rose’ and BA in M. ‘Lollipop’ occurred during the end flowering stage (S4) and flowering stage (S3) periods, respectively. The enzyme concentration change trends of phenylpyruvate decarboxylase (PDL), phenylacetaldehyde reductase (PAR), soluble protein, C, N, and C/N ratio changes during the S3-S4 period in M. ‘Praire Rose’ and M. ‘Lollipop’ were entirely opposite. Correlation and PCA analysis demonstrated that the content of CYP79D73 (a P450) and N, and the C/N ratio were key factors in 2-PE production in M. ‘Praire Rose’. The production of BA in M. ‘Lollipop’ was more influenced by the content of phenylacetaldehyde synthase (PAAS), CYP79D73, and soluble sugar. As CYP79D73 exits oppositely in correlation to 2-PE (M. ‘Praire Rose’) and BA (M. ‘Lollipop’), it is hypothesized that CYP79D73 was postulated as the primary factor contributing to the observed differences of 2-PE (M. ‘Praire Rose’) and BA (M. ‘Lollipop’) formation. These results carry significant implications for crabapple aromatic flower breeding and the essential oil industry etc.

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Biology of Floral Scent Volatiles in Ornamental Plants

Cited by 4 publications

References 47 publications

Comparative transcriptome analysis reveals an insight into the candidate genes involved in anthocyanin and scent volatiles biosynthesis in colour changing flowers of Combretum indicum

Comparative transcriptome analysis reveals an insight into the candidate genes involved in anthocyanin and scent volatiles biosynthesis in colour changing flowers of Combretum indicum

Floral scent emission ofEpiphyllum oxypetalum: identification of a novel cytosol-localized geraniol biosynthesis pathway

Physiological and Biochemical Analysis Revealing the Key Factors Influencing 2-Phenylethanol and Benzyl Alcohol Production in Crabapple Flowers

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