Biology of Bacterium Lepisepticum

Abstract

Inorganic iron substances of known chemical structure have been tested for their ability to function as the X factor in the growth of bacteria of the hemophilic, anaerobic) and hemorrhagic septicemia groups. Each iron compound possessing this biocatalytic activity showed oxygen-absorbing and peroxidase properties. The interrelation of these mechanisms is discussed.

Biology of Bacterium Lepisepticum

“…De Kruif noted that "D" strains, inoculated into broth or peptone solutions, gave rise to "G" variant forms after 48 to 72 hours incubation (2). Later, we made a more detailed study of the growth of this type (14,15), and noted the following facts: (1) that "D" strains, inoculated into aerobic meat infusion broth, pH 7.4, in quantifies less than 100,000 per cc., did not live; (2) that larger seedings multiplied after a period of lag, until a maximum number of 1,000,000,000 per cc. was reached; (3) that after 48 to 72 hours incubation, "G" variants appeared in increasing numbers, until they replaced entirely the original "D" forms.…”

Active Iron. I. The Structure of Werner Complexes of Metals of the Iron Family in Relation to the Chemical, Physical, and Biological Behavior of Their Central Metal Atoms.

“…The extensive experiments of Webster and Baudisch (79) showed that aquo salt diluted to 0.01 mg. per cubic centimeter can replace the X factor with hemophilic bacteria. Influenza bacilli also grew in the presence of aquo salt, as did B. welcjiii and B. sporogenes.…”