Biology, epidemiology and control of Fusicladium eriobotryae, causal agent of loquat scab

Abstract

Scab caused by the fungus Fusicladium eriobotryae is the most serious disease affecting loquat in Spain. Isolation of F. eriobotryae from infected tissue on culture media can be difficult due to its slow growth. A polymerase chain reaction (PCR) based protocol was developed for F. eriobotryae-specific identification from pure culture or infected loquat tissues. The primer set was designed in the elongation factor 1-α gene (EF1-α), and specificity and sensitivity for single and nested-PCR were validated. The nested-PCR assay resulted in 100% positive detection of F. eriobotryae in naturally and artificially infected tissues. This protocol can be useful for routine diagnosis, disease monitoring programs and epidemiological research.

Biology and Epidemiology of Venturia Species Affecting Fruit Crops: A Review

“…For (A) , the Web of Science was searched on 30 August 2016 for different combinations of words in the title (for apple: “ Venturia inaequalis” or “ Fusicladium pomi ” or “ Spilocaea pomi ” or “apple scab”; for pear: “ Venturia pyrina ” or “ Venturia pirina ” or “ Fusicladium pyrorum ” or “pear scab” and in light blue “ Venturia nashicola ” or “ Fusicladium nashicola ”; for pecan: “ Fusicladium effusum ” or “ Cladosporium effusum ” or “ Cladosporium caryigenum ” or ”pecan scab”; for olive “ Spilocaea oleagina ” or “ Fusicladium oleagineum ” or “olive scab” or “olive leaf spot”; for peach: “ Venturia carpophila ” or “ Fusicladium carpophilum ” or “ Cladosporium carpophilum ” or “peach scab”; and for loquat: “ Fusicladium eriobotryae ” or “ Spilocaea eriobotryae ” or “loquat scab”). Worldwide production data for (B) were extracted from FAOSTAT, except in the case of loquat, where data from González-Domínguez ( 2014 ) were used.…”