Biology, ecology and management of the South American tomato pinworm, Tuta absoluta.

Urbaneja, Alberto; Desneux, Nicolas; Gabarra, Rosa; Arnó, Judit; González‐Cabrera, Joel; Neto, Agenor Mafra; Stoltman, L.; Pinto, Alexandre de Sene; Parra, JosE Roberto Postali

doi:10.1079/9781845938291.0098

Cited by 37 publications

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“…The tomato leafminer is a multivoltine species whose young larvae can damage tomato plants during all developmental stages by forming large galleries in the leaves and burrowing into stalks, shoot apex, and green and ripe fruits. Tuta absoluta , which can cause yield losses of up to 100% in various regions and under diverse cultivation systems, has become the major tomato pest in South America and Africa (Desneux et al, 2010; Urbaneja et al, 2013; Campos et al, 2014; Tonnang et al, 2015). This insect invaded Europe in 2006 and spread to northern Africa in 2007 (Urbaneja et al, 2013), where it caused extensive economic losses to growers; multiple efforts have since been made to control this pest (Desneux et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

“…Tuta absoluta , which can cause yield losses of up to 100% in various regions and under diverse cultivation systems, has become the major tomato pest in South America and Africa (Desneux et al, 2010; Urbaneja et al, 2013; Campos et al, 2014; Tonnang et al, 2015). This insect invaded Europe in 2006 and spread to northern Africa in 2007 (Urbaneja et al, 2013), where it caused extensive economic losses to growers; multiple efforts have since been made to control this pest (Desneux et al, 2011). To aggravate this situation, resistance to insecticides has been reported for T. absoluta , making the development of alternative means of controlling this pest even more urgent (Urbaneja et al, 2013; Campos et al, 2014; Silva et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

“…This insect invaded Europe in 2006 and spread to northern Africa in 2007 (Urbaneja et al, 2013), where it caused extensive economic losses to growers; multiple efforts have since been made to control this pest (Desneux et al, 2011). To aggravate this situation, resistance to insecticides has been reported for T. absoluta , making the development of alternative means of controlling this pest even more urgent (Urbaneja et al, 2013; Campos et al, 2014; Silva et al, 2015). …”

Section: Introductionmentioning

confidence: 99%

“…Traditionally, target genes are screened and evaluated by adding dsRNA to artificial diets offered to insect larvae (Terenius et al, 2011; Zhang, Li & Miao, 2012; Wang et al, 2015; Fishilevich et al, 2016), but in the case of T. absoluta , there is no readily available artificial diet (Urbaneja et al, 2013). We therefore investigated two delivery approaches in which T. absoluta larvae feed on tomato leaflets containing dsRNA.…”

Section: Introductionmentioning

confidence: 99%

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RNA interference as a gene silencing tool to controlTuta absolutain tomato (Solanum lycopersicum)

Camargo

Barbosa

Possignolo

et al. 2016

PeerJ

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RNA interference (RNAi), a gene-silencing mechanism that involves providing double-stranded RNA molecules that match a specific target gene sequence, is now widely used in functional genetic studies. The potential application of RNAi-mediated control of agricultural insect pests has rapidly become evident. The production of transgenic plants expressing dsRNA molecules that target essential insect genes could provide a means of specific gene silencing in larvae that feed on these plants, resulting in larval phenotypes that range from loss of appetite to death. In this report, we show that the tomato leafminer (Tuta absoluta), a major threat to commercial tomato production, can be targeted by RNAi. We selected two target genes (Vacuolar ATPase-A and Arginine kinase) based on the RNAi response reported for these genes in other pest species. In view of the lack of an artificial diet for T. absoluta, we used two approaches to deliver dsRNA into tomato leaflets. The first approach was based on the uptake of dsRNA by leaflets and the second was based on “in planta-induced transient gene silencing” (PITGS), a well-established method for silencing plant genes, used here for the first time to deliver in planta-transcribed dsRNA to target insect genes. Tuta absoluta larvae that fed on leaves containing dsRNA of the target genes showed an ∼60% reduction in target gene transcript accumulation, an increase in larval mortality and less leaf damage. We then generated transgenic ‘Micro-Tom’ tomato plants that expressed hairpin sequences for both genes and observed a reduction in foliar damage by T. absoluta in these plants. Our results demonstrate the feasibility of RNAi as an alternative method for controlling this critical tomato pest.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

RNA interference as a gene silencing tool to controlTuta absolutain tomato (Solanum lycopersicum)

Camargo

Barbosa

Possignolo

et al. 2016

PeerJ

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“…It is based on the combination of preventive methods and monitoring of pests and their damage, but also on the use of biological, physical, and other sustainable non-chemical methods if they provide suitable pest control. Biological control (BC) which relies on the use of living organisms (natural enemies) to reduce pest populations is a key component of IPM [1,9,10]. It includes classical (introduction of natural enemies to a new area), augmentation (supplemental release of natural enemies), and conservation BC (habitat managed to favor natural enemies).…”

Section: Introductionmentioning

confidence: 99%

Life-History Traits of Macrolophus pygmaeus with Different Prey Foods

et al. 2016

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Macrolophus pygmaeus Rambur (Hemiptera: Miridae) is a generalist predatory mirid widely used in augmentative biological control of various insect pests in greenhouse tomato production in Europe, including the invasive tomato leafminer, Tuta absoluta (Meyrick) (Lepidoptera, Gelechiidae). However, its biocontrol efficacy often relies on the presence of alternative prey. The present study aimed at evaluating the effect of various prey foods (Ephestia kuehniella eggs, Bemisia tabaci nymphs, Tuta absoluta eggs and Macrosiphum euphorbiae nymphs) on some life history traits of M. pygmaeus. Both nymphal development and adult fertility of M. pygmaeus were significantly affected by prey food type, but not survival. Duration of nymphal stage was higher when M. pygmaeus fed on T. absoluta eggs compared to the other prey. Mean fertility of M. pygmaeus females was greatest when fed with B. tabaci nymphs, and was greater when offered M. euphorbiae aphids and E. kuehniella eggs than when offered T. absoluta eggs. Given the low quality of T. absoluta eggs, the efficacy of M. pygmaeus to control T. absoluta may be limited in the absence of other food sources. Experiments for assessing effectiveness of generalist predators should involve the possible impact of prey preference as well as a possible prey switching.

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Gene silencing by RNAi via oral delivery of dsRNA by bacteria in the South American tomato pinworm, Tuta absoluta

Bento

Marques

Campana

et al. 2019

Pest Management Science

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BACKGROUND RNA interference (RNAi) has been evaluated in several insect pests as a novel strategy to be included in integrated pest management. Lepidopterans are recognized to be recalcitrant to gene silencing by RNAi. As such, double‐stranded RNA (dsRNA) delivery needs to be adjusted to assure its stability until it reaches the target gene transcript for silencing. Gene silencing by RNAi offers the potential to be used in the control of Tuta absoluta (Meyrick), one of the main insect pests of tomato (Solanum lycopersicum) worldwide. Here, we tested the delivery of dsRNA expressed in Escherichia coli HT115(DE3) and supplied to larvae in an artificial diet by screening target genes for silencing. We tested six target genes: juvenile hormone inducible protein (JHP); juvenile hormone epoxide hydrolase protein (JHEH); ecdysteroid 25‐hydroxylase (PHM); chitin synthase A (CHI); carboxylesterase (COE); and arginine kinase (AK). RESULTS Based on larval mortality, the duration of the larval stage in days, pupal weight, and the accumulation of the target gene transcript, we demonstrated the efficacy of bacterial dsRNA delivery for the functional effects on larval development. Providing dsRNA targeted to JHP, CHI, COE and AK by bacteria led to a significant decrease in transcript accumulation and an increase in larval mortality. CONCLUSION Bacteria expressing dsRNA targeting essential T. absoluta genes supplied in artificial diet are efficient to screen RNAi target‐genes. The oral delivery of dsRNA by bacteria is a novel potential alternative for the control of T. absoluta based on RNAi. © 2019 Society of Chemical Industry

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Biology, ecology and management of the South American tomato pinworm, Tuta absoluta.

Cited by 37 publications

References 0 publications

RNA interference as a gene silencing tool to controlTuta absolutain tomato (Solanum lycopersicum)

RNA interference as a gene silencing tool to controlTuta absolutain tomato (Solanum lycopersicum)

Life-History Traits of Macrolophus pygmaeus with Different Prey Foods

Gene silencing by RNAi via oral delivery of dsRNA by bacteria in the South American tomato pinworm, Tuta absoluta

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