Biology and Industrial Potential of<i>Botryococcus braunii</i>

Watanabe, Makoto M.; Tanabe, Yuuhiko

doi:10.1002/9781118567166.ch19

Cited by 11 publications

(10 citation statements)

References 90 publications

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“…The highest biomass production for cultures receiving green light was at 14.22 W m −2 (22.0%). These results coincided with that reported by Watanabe et al 27 who found that growth was highest under red and lowest under green light; low productivity with green light may be due to poor absorption of light by chlorophyll and carotenoids, 18 i.e. A. obliquus absorbs the energy produced by violet, yellow and red light whilst the energy produced by the colour green is reflected.…”

Section: Experimental Conditionssupporting

confidence: 91%

Using different wavelengths and irradiance on the microalgae Acutodesmus obliquus batch culture

Hurtado

Garzón‐Castro

Cortés‐Romero

et al. 2019

J of Chemical Tech & Biotech

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BACKGROUND: The quality of light, such as wavelength and the intensity affect the performance of algal growth and lipid production. Moreover, these variables also affect the lipids profile. To study the interrelation among these variables, Acutodesmus obliquus (Scenedesmus obliquus UTEX393) a microalga, was cultivated using illumination by light-emitting-diode surface mount device extensions emitting red ( = 620-750 nm), yellow ( = 570-590 nm), green ( = 495-570 nm) and violet ( = 380-450 nm) light. Additionally, each culture was illuminated at different light intensities (I 1 = 8.75, I 2 = 14.22 and I 3 = 19.69 W m −2 ). Finally, all samples were analysed by GC-MS. RESULTS:The results showed: (1) the biomass production is directly proportional to the light intensity, e.g. at I 3 = 19.69 W m −2 violet (36.80 mg L −1 ), yellow (25.49 mg L −1 ) and red (39.44 mg L −1 ) showed the major production; (2) however, the lipids were produced in minor amounts at major violet light intensity (3.64 mg L −1 at I 3 = 19.69 W m −2 ); (3) the major lipids production (20.56 mg L −1 ) was obtained with red light at I 3 = 19.69 W m −2 and (4) A. obliquus, under the parameters established was a good producer of lipids. CONCLUSIONS:The major amount of biomass was obtained at red, yellow and violet light; meanwhile, the low biomass production, using green light, was associated with inefficient absorption of green light by chlorophyll. Additionally, palmitic, linolenic and linoleic were the most produced lipids, establishing a dependence between light wavelength and irradiance with biomass and lipid production. These results support the use of wavelengths and intensities to produce a group of specific lipids according to the needs of different industries.

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Section: Experimental Conditionssupporting

confidence: 91%

Using different wavelengths and irradiance on the microalgae Acutodesmus obliquus batch culture

Hurtado

Garzón‐Castro

Cortés‐Romero

et al. 2019

J of Chemical Tech & Biotech

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“…Botryococcus braunii Kützing is a colony-forming green alga, taxonomically belonging to the Trebouxiophyceae, Chlorophyta 22 . B. braunii has been recovered from fresh and brackish water environments, such as ponds, lakes, and reservoirs worldwide.…”

mentioning

confidence: 99%

A novel alphaproteobacterial ectosymbiont promotes the growth of the hydrocarbon-rich green alga Botryococcus braunii

Tanabe

Okazaki

Yoshida

et al. 2015

Sci Rep

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Botryococcus braunii is a colony-forming green alga that accumulates large amounts of liquid hydrocarbons within the colony. The utilization of B. braunii for biofuel production is however hindered by its low biomass productivity. Here we describe a novel bacterial ectosymbiont (BOTRYCO-2) that confers higher biomass productivity to B. braunii. 16S rDNA analysis indicated that the sequence of BOTRYCO-2 shows low similarity (<90%) to cultured bacterial species and located BOTRYCO-2 within a phylogenetic lineage consisting of uncultured alphaproteobacterial clones. Fluorescence in situ hybridization (FISH) studies and transmission electric microscopy indicated that BOTRYCO-2 is closely associated with B. braunii colonies. Interestingly, FISH analysis of a water bloom sample also found BOTRYCO-2 bacteria in close association with cyanobacterium Microcystis aeruginosa colonies, suggesting that BOTRYCO-2 relatives have high affinity to phytoplankton colonies. A PCR survey of algal bloom samples revealed that the BOTRYCO-2 lineage is commonly found in Microcystis associated blooms. Growth experiments indicated that B. braunii Ba10 can grow faster and has a higher biomass (1.8-fold) and hydrocarbon (1.5-fold) yield in the presence of BOTRYCO-2. Additionally, BOTRYCO-2 conferred a higher biomass yield to BOT-22, one of the fastest growing strains of B. braunii. We propose the species name ‘Candidatus Phycosocius bacilliformis’ for BOTRYCO-2.

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“…Myers also conducted experiments on the effects of high-light intensity on the photosynthesis of Chlorella (Myers and Burr, 1940). At the same time, studies on the effects of nitrogen and phosphate on the growth medium of algae were done by Chu (1942) using several species such as Botryococcus braunii (K€ utzing, 1849), a promising algal hydrocarbon feedstock (Watanabe and Tanabe, 2013).…”

Section: Microalgae a Milestone In Light To Fuel Conceptmentioning

confidence: 99%

From the Ancient Tribes to Modern Societies, Microalgae Evolution from a Simple Food to an Alternative Fuel Source

Öncel

Köse

Vardar

et al. 2015

Handbook of Marine Microalgae

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Biology and Industrial Potential ofBotryococcus braunii

Cited by 11 publications

References 90 publications

Using different wavelengths and irradiance on the microalgae Acutodesmus obliquus batch culture

Using different wavelengths and irradiance on the microalgae Acutodesmus obliquus batch culture

A novel alphaproteobacterial ectosymbiont promotes the growth of the hydrocarbon-rich green alga Botryococcus braunii

From the Ancient Tribes to Modern Societies, Microalgae Evolution from a Simple Food to an Alternative Fuel Source

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