Biology and Diseases of Birds

Bryant, E S

doi:10.1016/b978-0-12-263620-2.50019-7

Cited by 2 publications

(1 citation statement)

References 8 publications

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“…We did not ¢nd evidence of infection in the embryonic lungs which, before the egg hatches, are buds of tissue only 3^4 mm long that have not yet begun to function in breathing. In adult birds, M. avium infections cause nodules in the intestinal tract and granulomas in the liver, spleen and bone marrow; granulomas are seldom found in the lungs [2]. The question remains if the egg su¤ciently mimics the conditions found in the immunocompromised human to the extent that the bacterial genes responsible for human disease can be di¡erentially expressed and identi¢ed using this model system.…”

Section: Discussionmentioning

confidence: 99%

Changes in the virulence of Mycobacterium avium after passage through embryonated hens' eggs

Long

2000

FEMS Microbiology Letters

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Eight-day-old embryonated hen's eggs were used as a model to study Mycobacterium avium virulence. Strains isolated from human patients caused 20^90% mortality when eggs were infected by injection of bacterial suspensions into the amniotic sac. Virulence of examined strains subsequently decreased with passage through eggs to between 0 and 40% mortality in four passages. Virulence of the egg-attenuated strains could be restored by passage through human peripheral blood mononuclear cells. The site of infection in the egg was usually the mesodermal layer of the chorioallantoic membrane. A few small granulomas containing acid-fast bacteria were seen in the liver, but not in other organs. Death of chicken embryos may have resulted from destruction of the mesodermal layer of the chorioallantoic membrane with consequent respiratory failure. PBMCs infected with less virulent egg-passaged strains of M. avium produced higher levels of tumor necrosis factor-K than did peripheral blood mononuclear cells infected with more virulent nonpassaged strains. ß

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Section: Discussionmentioning

confidence: 99%

Changes in the virulence of Mycobacterium avium after passage through embryonated hens' eggs

Long

2000

FEMS Microbiology Letters

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show abstract

Changes in the virulence ofMycobacterium aviumafter passage through embryonated hensâ eggs

Long

Ewing

Bartlett

et al. 2000

FEMS Microbiology Letters

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Eight-day-old embryonated hen's eggs were used as a model to study Mycobacterium avium virulence. Strains isolated from human patients caused 20-90% mortality when eggs were infected by injection of bacterial suspensions into the amniotic sac. Virulence of examined strains subsequently decreased with passage through eggs to between 0 and 40% mortality in four passages. Virulence of the egg-attenuated strains could be restored by passage through human peripheral blood mononuclear cells. The site of infection in the egg was usually the mesodermal layer of the chorioallantoic membrane. A few small granulomas containing acid-fast bacteria were seen in the liver, but not in other organs. Death of chicken embryos may have resulted from destruction of the mesodermal layer of the chorioallantoic membrane with consequent respiratory failure. PBMCs infected with less virulent egg-passaged strains of M. avium produced higher levels of tumor necrosis factor-alpha than did peripheral blood mononuclear cells infected with more virulent nonpassaged strains.

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Biology and Diseases of Birds

Cited by 2 publications

References 8 publications

Changes in the virulence of Mycobacterium avium after passage through embryonated hens' eggs

Changes in the virulence of Mycobacterium avium after passage through embryonated hens' eggs

Changes in the virulence ofMycobacterium aviumafter passage through embryonated hensâ eggs

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Biology and Diseases of Birds

Cited by 2 publications

References 8 publications

Changes in the virulence of Mycobacterium avium after passage through embryonated hens' eggs

Changes in the virulence of Mycobacterium avium after passage through embryonated hens' eggs

Changes in the virulence ofMycobacterium aviumafter passage through embryonated hensâ eggs

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Changes in the virulence ofMycobacterium aviumafter passage through embryonated hensâ eggs