Biology and Chemistry of Endophytes

Zhang, Huawei; Song, Yong; Tan, Ren Xiang

doi:10.1002/chin.200703269

Cited by 97 publications

(119 citation statements)

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“…Isolation and characterization of pharmacologically active compounds from medicinal plants is continuing even today. All the plants have mutualistic internal endophytic fungi [22][23] . "Endophytes are fungi or bacteria occurring inside plant tissues without causing any apparent symptoms in the host".…”

Section: Dpph Assaymentioning

confidence: 99%

Bio Diversity of Endophytic Mycobiota From Limonia ACIDISSIMA.L and Screening of Antioxidant Activity

Kannan¹,

Poovizhi²,

Priyadharshini³

et al. 2017

Int. Res. J. Pharm.

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The present study was carried out to isolate and identify the endophytic fungi from different plant parts (Leaves, Twig, and Shoot) of Limonia acidissima L. and to screening the antioxidant activity. The plants samples were collected and processed within 24 hours with modified surface sterilization. The plants segments were placed on PDA incubated for four weeks. The emerging fungal colonies were sub-cultured and identified morphologically and microscopically. The present study proves that the plant segments of endophytic fungi colonized 150 Segments were screened from leaves, twigs and shoot of selected medicinal plant. A total of 34 isolates were obtained. Among them 7 were classified into sterile morphospecies, remaining were classified into 28 species which belongs to 11 genera. The predominant endophytic fungi were Phyllosticta Sp., Phoma Sp., Nigrospora sphaerica, Alternaria alternata, Dreschlera australiensis, Sterile forms. The phytochemicals present in the Limonia acidissima L. were Tannins, Saponins, Terpenoids, Cardiac glycosides, Steroids, Flavonoids and Phenols. The antioxidant of free radical scavenging activity was recorded around 99% inhibition at the concentration of 25mg/ml. The Limonia acidissima L. were 100% of endophytic fungal infection and also the endophytes are effectively free radical scavenging activity and the R square values are significant. The microbes are fast producers for natural products and it makes excellent drugs for curing many diseases.

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Section: Dpph Assaymentioning

confidence: 99%

Bio Diversity of Endophytic Mycobiota From Limonia ACIDISSIMA.L and Screening of Antioxidant Activity

Kannan¹,

Poovizhi²,

Priyadharshini³

et al. 2017

Int. Res. J. Pharm.

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“…Endolichenic fungi, an emerging group of endosymbiotic microorganisms, consist of fungal strains that live within asymptomatic lichen thalli, much in the same way as endophytic fungi live within healthy plant tissues (1). In contrast to endophytic fungi that have been studied chemically a number of times (2)(3)(4), only a limited number of endolichenic fungi have thus far been investigated for their secondary metabolites (5,6), but studies have shown that endolichenic fungi are also rich sources of structurally diverse and biologically active small-molecule metabolites. In the course of the current authors' ongoing efforts to discover bioactive compounds from endophytic and endolichenic microorganisms, four new anthraquinone derivatives (1-4) ( Figure 1) were obtained, along with six known anthraquinones (5-10) and three xanthones (11)(12)(13) (Supplemental data, http://www.…”

Section: Introductionmentioning

confidence: 99%

Metabolites from Aspergillus versicolor, an endolichenic fungus from the lichen Lobaria retigera

Dou

Wang

Jiang

et al. 2014

DD&T

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“…IR data were recorded using a Shimadzu FTIR-8400S spectrophotometer. 1 Cytochalasans from endophytic fungus Trichoderma gamsii G Ding et al C-19-C-20, C-11-C-5-C-4-C-3-C-10-C-22/C-23, and C-7-C-8-C-13 fragments (Figure 2), and the remaining connection was completely established by Heteronuclear multiple-bond correlations (Figure 2). The correlations from H-3 to C-1, H-4 to C-1 and C-9, and 2-NH-to C-1, C-3, C-4 and C-9 established a pyrrolidin-2-one ring system with one isobutyl connected at C-3.…”

mentioning

confidence: 99%

“…1 Thus, endophytes are considered an outstanding source of bioactive natural products, and many different secondary metabolites with diverse structural features have been isolated from endophytes. [1][2][3][4][5][6] During an ongoing search for new bioactive secondary metabolites from endophytic fungi, the extract of one endophytic fungus Trichoderma gamsii inhabiting the traditional Chinese medicinal plant Panax notoginseng (BurK.) F.H.Chen displayed cytotoxic activity against HeLa cells.…”

mentioning

confidence: 99%

Cytochalasans with different amino-acid origin from the plant endophytic fungus Trichoderma gamsii

et al. 2012

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Endophytes, as members of special niche of fungi inhabiting unique environments, namely, the normal plant tissues or organs, have acquired genetic and species diversities, which could transform into chemo-structural and bioactive diversities. 1 Thus, endophytes are considered an outstanding source of bioactive natural products, and many different secondary metabolites with diverse structural features have been isolated from endophytes. [1][2][3][4][5][6] During an ongoing search for new bioactive secondary metabolites from endophytic fungi, the extract of one endophytic fungus Trichoderma gamsii inhabiting the traditional Chinese medicinal plant Panax notoginseng (BurK.) F.H.Chen displayed cytotoxic activity against HeLa cells. Bioactivityguided separation of this extract, resulted in two new cytochalasans, trichalasin A (1) originated from valine, and trichalasin B (2) together with aspochalasins I (3) 7 and J (4) 7 derived from leucine ( Figure 1). In this note, we present the isolation, structural elucidation and bioactivity of these compounds.The culture of T. gamsii was isolated from traditional Chinese medicinal plant P. notoginseng (BurK.) F.H.Chen. The isolate was identified based on the sequence (Genbank Accession No. JF964996) obtained the by analysis of ITS region of the rDNA. The fungal strain was cultured on the slants of potato dextrose agar at 25 1C for 10 days. The agar plugs were used to inoculate 250-ml Erlenmeyer flasks, each containing 40 ml of media (0.4% glucose, 1% malt extract and 0.4% yeast extract), and the final pH of the media was adjusted to 6.5 before sterilization. Flask cultures were incubated at 25 1C on a rotary shaker at 170 r.p.m. for 5 days. Fermentation was carried out in Fernbach flasks (500 ml) each containing 80 g of rice. Spore inoculum was prepared by suspension in sterile, distilled H 2 O to give a final spore/cell suspension of 1Â10 6 per ml. Distilled H 2 O (100 ml) was added to each flask, and the contents were soaked overnight before autoclaving at 15 lb in À2 for 30 min. After cooling to room temperature, each flask was inoculated with 5 ml of the spore inoculum and incubated at 25 1C for 40 days.The fermented material was extracted with ethyl acetate (20 l), and the organic solvent was evaporated to dryness under vacuum to afford a crude extract (100 g), which was fractionated by silica gel column chromatography (10Â100 cm) using CH 2 Cl 2 -CH 3 OH gradient elution. The fraction (0.8 g) which eluted with 100:2 CH 2 Cl 2 -CH 3 OH was separated by column chromatography, and further purification by reversed-phase HPLC (Lumtech, Berlin, Germany; YMC-Pack ODS-A column; 10 mm; 250Â10 mm; 2 ml min À1 , 75% MeOH in H 2 O for 5 min, and followed by 75-90% for 60 min) afforded trichalasin A (1; 1.5 mg, t R 21.5 min) and aspochalasin J (4; 5 mg, t R 29.3 min). Other fraction (0.3 g) eluted with 100:2 CH 2 Cl 2 -CH 3 OH was separated by Sephadex LH-20 CC repeatedly to obtain trichalasin B (2; 2 mg). One fraction eluted with 100:8 CH 2 Cl 2 -CH 3 OH was separated by Sephadex ...

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Biology and Chemistry of Endophytes

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Cited by 97 publications

References 1 publication

Bio Diversity of Endophytic Mycobiota From Limonia ACIDISSIMA.L and Screening of Antioxidant Activity

Bio Diversity of Endophytic Mycobiota From Limonia ACIDISSIMA.L and Screening of Antioxidant Activity

Metabolites from Aspergillus versicolor, an endolichenic fungus from the lichen Lobaria retigera

Cytochalasans with different amino-acid origin from the plant endophytic fungus Trichoderma gamsii

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