Biology and biochemistry of sexual and sporogenic stages of <i>Plasmodium falciparum</i>: a review

Meuwissen, J. H. E. T.; Ponnudurai, T.

doi:10.1016/0248-4900(88)90083-4

Cited by 7 publications

(2 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The demonstrated parasite transmission to the ticks implies that B. divergens infective stages are produced in vitro in the described culture conditions. This is an important feature as it is very difficult to obtain sexual stages infective for the vector for other closely related vector-transmitted haemoprotozoan such as Plasmodium falciparum (Meuwissen and Ponnudurai, 1988) and because it is suspected that parasites artificially maintained in vitro may lose their infectiousness capacity (Stewart et al 1986).…”

Section: Discussionmentioning

confidence: 99%

Transstadial and transovarial persistence ofBabesia divergensDNA inIxodes ricinusticks fed on infected blood in a new skin-feeding technique

et al. 2006

View full text Add to dashboard Cite

Although Babesia divergens is the the principal confirmed zoonotic Babesia sp. in Europe, there are gaps in our knowledge of its biology and transmission by the tick Ixodes ricinus. In order to reproduce the part of the parasite cycle that occurs in the vector, an in vitro animal skin feeding technique on blood containing in vitro cultivated B. divergens was developed. Parasite DNA was detected in all samples of salivary glands of nymphs and adults that had fed on parasitized blood as larvae and nymphs, respectively, indicating acquisition as well as a transtadial persistence of B. divergens. PCR performed on eggs and larvae produced by females that had fed on parasitized blood demonstrated the existence of a transovarial transmission of the parasite. Gorging B. divergens infected larvae on non-infected gerbils showed persistance of the parasite over moulting into the resulting nymphs. These results indicate that the parasitic stages infective for the vector (i.e. the sexual stages) can be produced in vitro. To our knowledge, this is the first report of artificial feeding of I. ricinus via membrane as well as in vitro transmission of B. divergens to its vector. The opportunities offered by the use of such a transmission model of a pathogen by I. ricinus are discussed.

show abstract

Section: Discussionmentioning

confidence: 99%

Transstadial and transovarial persistence ofBabesia divergensDNA inIxodes ricinusticks fed on infected blood in a new skin-feeding technique

et al. 2006

View full text Add to dashboard Cite

show abstract

“…Printed in the United Kingdom © 1998 Cambridge University Press been reviewed on numerous occasions (Sinden, 1981(Sinden, , 1983aAikawa et al 1984;Meuwissen & Ponnudurai, 1988), and a book has been written about the biology of the insect midgut (Lehane & Billingsley, 1996). This article attempts to discuss the development of the Plasmodium ookinete and the physical and chemical factors in the mosquito midgut that may influence such development.…”

Section: Introductionmentioning

confidence: 98%

Plasmodiumookinete development in the mosquito midgut: a case of reciprocal manipulation

Shahabuddin

1998

Parasitology

View full text Add to dashboard Cite

The ookinete is one of the most important stages of Plasmodium development in the mosquito. It is morphologically and biochemically distinct from the earlier sexual stages--gametocytes and zygote, and from the later stages--oocyst and sporozoites. Development to ookinete allows the parasite to escape from the tightly packed blood bolus, to cross the sturdy peritrophic matrix (PM), to be protected from the digestive environment of the midgut lumen, and to invade the gut epithelium. The success of each of these activities may depend on the degree of the biochemical and physical barriers in the mosquito (such as density of blood bolus, thickness of peritrophic matrix, proteolytic activities in the gut lumen etc.) and the ability of the ookinete to overcome these barriers. Ookinete motility, secretion of chitinase, resistance to the digestive enzymes, and recognition/invasion of the midgut epithelium all may play crucial roles in the transformation to oocyst. The overall sporogonic development of Plasmodium, therefore, depends on the results of the two-way manipulations between the parasite and the vector mosquito. Study of ookinete development and of the cellular and biochemical complexities of the mosquito gut may therefore lead to the design of novel strategies to block the transmission of malaria. This article reviews the intricate interactions between the parasite and the mosquito midgut in the context of development and transmission of Plasmodium parasites.

show abstract

Plasmodium falciparum ookinetes migrate intercellularly throughAnopheles stephensi midgut epithelium

et al. 1989

View full text Add to dashboard Cite

The migration of Plasmodium falciparum and P. berghei ookinetes through the midgut epithelium in Anopheles stephensi was studied by transmission electron microscopy. With ruthenium red (RR) staining, the results of previous studies were confirmed: P. falciparum ookinetes take an intercellular route through the midgut epithelium. In the same mosquito species, the rodent parasite P. berghei appeared to take an intracellular position, as previously suggested by other authors. The intra- or intercellular ookinete migration of P. berghei or P. falciparum, respectively, can perhaps be related to the higher mortality of P. berghei-infected mosquitoes within the first 2 days of infection. Evidence is presented that oocyst capsule formation begins as early as during the migration of the ookinete. After localization between the epithelial cells and the midgut basal lamina, the rapidly expanding oocyst stretches the overlying layer of the latter at the haemocoelic surface while a new basal lamina is generated between the oocyst and epithelial cell.

show abstract

Biology and biochemistry of sexual and sporogenic stages of Plasmodium falciparum: a review

Cited by 7 publications

References 17 publications

Transstadial and transovarial persistence ofBabesia divergensDNA inIxodes ricinusticks fed on infected blood in a new skin-feeding technique

Transstadial and transovarial persistence ofBabesia divergensDNA inIxodes ricinusticks fed on infected blood in a new skin-feeding technique

Plasmodiumookinete development in the mosquito midgut: a case of reciprocal manipulation

Plasmodium falciparum ookinetes migrate intercellularly throughAnopheles stephensi midgut epithelium

Contact Info

Product

Resources

About