Biologically inspired growth of hydroxyapatite nanocrystals inside self-assembled collagen fibers

Roveri, Norberto; Falini, Giuseppe; Sidoti, M. C.; Tampieri, Anna; Landi, Elena; Sandri, Monica; Parma, B.

doi:10.1016/s0928-4931(02)00318-1

Cited by 136 publications

(81 citation statements)

References 18 publications

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“…Results of the ICP analysis of the HA/Coll composites are reported in Table 1 oriented parallel to the direction of orientation of the fibers [14, 24,25]. In the case of the composite containing Mg-HA, the XRD pattern (Fig.…”

Section: Development and Characterization Of The Lower (Bony) And Intmentioning

confidence: 99%

Design of graded biomimetic osteochondral composite scaffolds

et al. 2008

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With the ultimate goal to generate suitable materials for the repair of osteochondral defects, in this work we aimed at developing composite osteochondral scaffolds organized in different integrated layers, with features which are biomimetic for articular cartilage and subchondral bone and can differentially support formation of such tissues.A biologically inspired mineralization process was first developed to nucleate Mg-doped hydroxyapatite crystals on type I collagen fibers during their self assembling. The resulting mineral phase was non-stoichiometric and amorphous, resembling chemico-physical features of newly deposited, natural bone matrix. A graded material was then generated, consisting of (i) a lower layer of the developed biomineralized collagen, corresponding to the subchondral bone, (ii) an upper layer of hyaluronic acid-charged collagen, mimicking the cartilaginous region, and (iii) an intermediate layer of the same nature as the biomineralized collagen, but with a lower extent of mineral, resembling the tidemark. The layers were stacked and freeze-dried, to obtain an integrated, monolithic composite. Culture of the material for 2 weeks after loading with articular chondrocytes yielded cartilaginous tissue formation selectively in the upper layer. Conversely, ectopic implantation in nude mice of the material after loading with bone marrow stromal cells resulted in bone formation which remained confined within the lower layer.In conclusion, we developed a composite material with cues which are biomimetic of an osteochondral tissue and with the capacity to differentially support cartilage and bone tissue generation. The results warrant test of the material as a substitute for the repair of osteochondral lesions in orthotopic animal models. Response to Reviewer #1:1) results and discussion should be separated We would prefer to maintain Results and Discussion combined, in order to avoid possible redundancies and improve readability. 2) figures should be detailed with arrows and information about specific orientations. Details and arrows have been included in the Figures3) collagen type 1 was used for the creation of the construct -in every layer? Usually collagen type 1 does not play a role in articular cartilage. This should be addressed in the discussion. According to the Reviewer's comment, a rationale for the use of type I collagen has been included in the text, as follows: "Type I collagen was selected for the synthesis of the composite layers, including the cartilaginous one, due to (i) its good physico-chemical stability and processability and (ii) high safety and biocompatibility profile, related to the removal of all Reply Letter to Editor and referee's comments telopeptides, which are potentially responsible for immunological reactions." (Section 2.1, Page 2, 1 st paragraph). Indeed, type II collagen is normally extracted porcine derivative, and is still not available either in bulk for processing or for clinical use. Nowadays there are only few papers on experimental studies desc...

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Section: Development and Characterization Of The Lower (Bony) And Intmentioning

confidence: 99%

Design of graded biomimetic osteochondral composite scaffolds

et al. 2008

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“…Although to date, neither the elegance of the biomineral assembly mechanisms nor the intricate composite nano-architectures have been duplicated by non-biological methods, the best way to mimic bone is to copy the way it is formed, namely by nucleation and growth of CDHA nanocrystals from a supersaturated solution both onto and within the collagen fibrils [591][592][593]. Such syntheses were denoted as ''biologically inspired'' which means they reproduce an ordered pattern and an environment very similar to natural ones [594][595][596]. The biologically inspired biocomposites of collagen and calcium orthophosphates (mainly, apatites) for bone substitute have a long history [29,364,499,[597][598][599][600][601][602][603][604][605][606][607][608][609][610][611][612][613][614][615] and started from the pioneering study by Mittelmeier and Nizard [616], who mixed calcium orthophosphate granules with a collagen web.…”

Section: Biocomposites With Collagenmentioning

confidence: 99%

“…-containing solution to allow mineral deposition. Also, collagen I fibers might be dissolved in acetic acid and then this solution is added to phosphoric acid, followed by the neutralization synthesis (performed at 25°C and solution pH within 9-10) between an aqueous suspension of Ca(OH) 2 and the H 3 PO 4 /collagen solution [594,595]. In order to ensure the quality of the final product, it is necessary to control the Ca/P ionic ratio in the reaction solution.…”

Section: Biocomposites With Collagenmentioning

confidence: 99%

“…5 [628]. Another proof of a chemical interaction between CDHA and collagen fibers was also evaluated in FTIR spectra of CDHA/collagen biocomposites, in which a shift of the band corresponding to -COO -stretching from 1340 to 1337 cm -1 was observed [594,595]. More to the point, nucleation of CDHA crystals onto collagen through a chemical interaction with carboxylate Reprinted from Ref.…”

Section: Biosensorsmentioning

confidence: 99%

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Calcium orthophosphate-based biocomposites and hybrid biomaterials

Dorozhkin¹

2009

J Mater Sci

285

146

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“…An alternative soaking process in calcium and phosphorous solutions provided the HAp formation in a three-dimensionally structured organic hydrogel matrix at ambient temperature [18,19]. An ordered composite powder of HAp and organic molecules was also prepared through self-organization by interplay at the organic-inorganic interface [20][21][22][23][24][25][26][27][28]. A composite material with a hierarchical structure was fabricated by the self-assembly of nanofibrils of mineralized collagen [29].…”

Section: Introductionmentioning

confidence: 99%

Preparation of hierarchically organized calcium phosphate–organic polymer composites by calcification of hydrogel

Furuichi

Oaki

Ichimiya

et al. 2006

Science and Technology of Advanced Materials

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A novel type of calcium phosphate-organic polymer composite having a hierarchical structure was prepared by calcification of a poly(acrylic acid) hydrogel. Macroscopically, an organic gel containing phosphate ions was transformed into an opaque solid material by diffusion of calcium ions. We observed the formation of micrometer-scale layered structures consisting of nanoscale crystals of hydroxylapatite (HAp) in the opaque products. The laminated architecture resulting from the periodic precipitation of calcium phosphate varied with the reaction conditions, such as the concentrations of the precursor ions and the density of the gel. The nanoscopic structure of HAp crystals was modified by the addition of gelatin to the polymer matrix. q

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Biologically inspired growth of hydroxyapatite nanocrystals inside self-assembled collagen fibers

Cited by 136 publications

References 18 publications

Design of graded biomimetic osteochondral composite scaffolds

Design of graded biomimetic osteochondral composite scaffolds

Calcium orthophosphate-based biocomposites and hybrid biomaterials

Preparation of hierarchically organized calcium phosphate–organic polymer composites by calcification of hydrogel

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