Biologically and mechanically driven design of an RGD-mimetic macroporous foam for adipose tissue engineering applications

Rossi, Eleonora; Gerges, Irini; Tocchio, Alessandro; Tamplenizza, Margherita; Aprile, Paola; Recordati, Camilla; Martello, Federico; Martin, Ivan; Milani, P.; Lenardi, Cristina

doi:10.1016/j.biomaterials.2016.07.004

Cited by 35 publications

(13 citation statements)

References 37 publications

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“…The large-size and round porosity adequate for adipocyte colonization and the isotropic structure of apple hypanthium present interesting similarities with human AT, and mechanical investigation confirmed its suitability in mimicking the mechanical features of the native AT (E apple = 4 kPa vs. E adipose tissue = 2–20 kPa) ( Omidi et al, 2014 ; Van Nieuwenhove et al, 2017 ). The decellularized scaffolds also show mechanical features comparable to those of other AT scaffolds proposed in literature ( Davidenko et al, 2010 ; Yu et al, 2013 ; Rossi et al, 2016 ). Carrot-derived scaffolds are characterized by a smaller and heterogeneous porosity, related to higher mechanical properties and lower water adsorption, compared to the apple structures.…”

Section: Discussionsupporting

confidence: 78%

“…Samples were tested up to 30% strain to replicate AT physiological conditions ( Frydrych et al, 2015 ) and were able to sustain this deformation without failure. Hydrated decellularized apple-derived samples were characterized by a compression modulus of 4.17 ± 0.17 kPa; this is comparable to that of native human adipose tissue [e.g., E breast tissue ( Van Nieuwenhove et al, 2017 ) = 2 kPa, E abdomen tissue ( Omidi et al, 2014 ) = 3.3 kPa] and to those of recently proposed scaffolds for AT regeneration, including polyamidoamine foams ( Rossi et al, 2016 ) ( E = 3.4–4.4 kPa), decellularized AT ( Yu et al, 2013 ) ( E = 2.4–4 kPa) and collagen-hyaluronic acid scaffolds ( Davidenko et al, 2010 ) ( E = 5.39–6.73 kPa). No statistical difference was observed between the elastic moduli of decellularized and control samples ( Table 1 ).…”

Section: Resultsmentioning

confidence: 67%

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Plant Tissues as 3D Natural Scaffolds for Adipose, Bone and Tendon Tissue Regeneration

Negrini

Toffoletto

Altomare

2020

Front. Bioeng. Biotechnol.

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Decellularized tissues are a valid alternative as tissue engineering scaffolds, thanks to the three-dimensional structure that mimics native tissues to be regenerated and the biomimetic microenvironment for cells and tissues growth. Despite decellularized animal tissues have long been used, plant tissue decellularized scaffolds might overcome availability issues, high costs and ethical concerns related to the use of animal sources. The wide range of features covered by different plants offers a unique opportunity for the development of tissue-specific scaffolds, depending on the morphological, physical and mechanical peculiarities of each plant. Herein, three different plant tissues (i.e., apple, carrot, and celery) were decellularized and, according to their peculiar properties (i.e., porosity, mechanical properties), addressed to regeneration of adipose tissue, bone tissue and tendons, respectively. Decellularized apple, carrot and celery maintained their porous structure, with pores ranging from 70 to 420 µm, depending on the plant source, and were stable in PBS at 37 • C up to 7 weeks. Different mechanical properties (i.e., E apple = 4 kPa, E carrot = 43 kPa, E celery = 590 kPa) were measured and no indirect cytotoxic effects were demonstrated in vitro after plants decellularization. After coating with poly-L-lysine, apples supported 3T3-L1 preadipocytes adhesion, proliferation and adipogenic differentiation; carrots supported MC3T3-E1 pre-osteoblasts adhesion, proliferation and osteogenic differentiation; celery supported L929 cells adhesion, proliferation and guided anisotropic cells orientation. The versatile features of decellularized plant tissues and their potential for the regeneration of different tissues are proved in this work.

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Section: Discussionsupporting

confidence: 78%

Section: Resultsmentioning

confidence: 67%

Plant Tissues as 3D Natural Scaffolds for Adipose, Bone and Tendon Tissue Regeneration

Negrini

Toffoletto

Altomare

2020

Front. Bioeng. Biotechnol.

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“…The combination of adult stem cells from various sources within 3D polymeric structures has been one of the most approached to generate 3D adipose-like tissues. The majority of these works have been using 3T3-L1 [ 4 , 5 , 6 , 7 ], a murine pre-adipocyte cell line that has limited representation of human tissue physiology. In alternative, adult stem cells have been differentiated into the adipogenic lineage in a range of natural/synthetic materials [ 8 , 9 , 10 , 11 ].…”

Section: Introductionmentioning

confidence: 99%

Generation of Gellan Gum-Based Adipose-Like Microtissues

et al. 2018

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Adipose tissue is involved in many physiological processes. Therefore, the need for adipose tissue-like analogues either for soft tissue reconstruction or as in vitro testing platforms is undeniable. In this work, we explored the natural features of gellan gum (GG) to recreate injectable stable adipose-like microtissues. GG hydrogel particles with different percentages of polymer (0.5%, 0.75%, 1.25%) were developed and the effect of obtained mechanical properties over the ability of hASCs to differentiate towards the adipogenic lineage was evaluated based on the expression of the early (PPARγ) and late (FABP4) adipogenic markers, and on lipids formation and accumulation. Constructs were cultured in adipogenic induction medium up to 21 days or for six days in induction plus nine days in maintenance media. Overall, no significant differences were observed in terms of hASCs adipogenic differentiation within the range of Young’s moduli between 2.7 and 12.9 kPa. The long-term (up to six weeks) stability of the developed constructs supported its application in soft tissue reconstruction. Moreover, their ability to function as adipose-like microtissue models for drug screening was demonstrated by confirming its sensitivity to TNFα and ROCK inhibitor, respectively involved in the repression and induction of the adipogenic differentiation.

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“…However, bioactive properties can be incorporated into other materials through presentation of peptides (e.g., RGD to promote adhesion) from a hydrogel backbone. [327] Injectable biomaterials and 3D porous implantable scaffolds are commonly used to deliver cells for adipose tissue regeneration. Injectable materials are advantageous because they allow minimally invasive delivery, can conform to any tissue geometry, and can gel in situ.…”

Section: Adipose Tissue Biomaterialsmentioning

confidence: 99%

Biomaterials to Mimic and Heal Connective Tissues

2019

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Connective tissue is one of the four major types of animal tissue and plays essential roles throughout the human body. Genetic factors, aging, and trauma all contribute to connective tissue dysfunction and motivate the need for strategies to promote healing and regeneration. The goal of this Review is to link a fundamental understanding of connective tissues and their multiscale properties to better inform the design and translation of novel biomaterials to promote their regeneration. We discuss major clinical problems in adipose tissue, cartilage, dermis, and tendon that inspire the need to replace native connective tissue with biomaterials. We then detail multiscale structure-function relationships in native soft connective tissues that may be used to guide material design. Several biomaterials strategies to improve healing of these tissues that incorporate biologics and are biologic-free are reviewed. Finally, we highlight important guidance documents and standards (ASTM, FDA, EMA) that are important to consider for translating new biomaterials into clinical practice.

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Biologically and mechanically driven design of an RGD-mimetic macroporous foam for adipose tissue engineering applications

Cited by 35 publications

References 37 publications

Plant Tissues as 3D Natural Scaffolds for Adipose, Bone and Tendon Tissue Regeneration

Plant Tissues as 3D Natural Scaffolds for Adipose, Bone and Tendon Tissue Regeneration

Generation of Gellan Gum-Based Adipose-Like Microtissues

Biomaterials to Mimic and Heal Connective Tissues

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