Biologically Active Transcripts of Beet Necrotic Yellow Vein Virus RNA-3 and RNA-4

Synthesis of infectious transcripts of blueberry scorch carlavirus in vitro

“…T4 transcripts, systemic symptoms identical to those produced by wild-type virus infection were observed. As shown in Table 1, pBS.T4 transcripts were far more infectious when capped, a similar result to those reported for in vitro synthesized infectious transcripts of the potexviruses, potato virus X (Hemenway et al, 1990), white clover mosaic virus (WC1MV) (Beck et al, 1990), clover yellow mosaic virus (CYMV) (Holy & AbouHaidar, 1993), papaya mosaic virus (PMV) (Sit & AbouHaidar, 1993) and the furovirus BNYVV (Zeigler-Graff et al, 1988). This result supports the conclusion that native BBScV RNA is capped as predicted from primer extension analysis (Cavileer et al, 1994).…”

“…In vitro synthesized transcripts of the potexviruses, WC1MV, CYMV and PMV have shown that as the length of the poly(A) tail is decreased, reduction in infectivity is observed Holy & AbouHaidar, 1993;Sit & AbouHaidar, 1993). The presence of additional ribonucleotides at the 3' terminus of the transcript did not appear to affect infectivity of the BBScV transcripts, a result similar to those reported for other poly(A)-containing virus genomes (Zeigler-Graff et al, 1988;Eggen et al, 1989;Beck et al, 1990).…”

In vitro synthesis of biologically active transcripts of tomato black ring virus satellite RNA

“…As with transcripts of other cloned cDNA of polyadenylated virus RNAs (van der Werfet al, 1986;Vos et al, 1988;Ziegler-Graff et al, 1988), multiplication of TBRV satRNA transcripts occurred with different lengths of extra viral nucleotides at the 3' end, and these extra nucleotides were not maintained in the progeny RNA. This suggests that the poly(A) stretch might be the signal (or a part of it) for the recognition of plus-strand template RNA.…”

High resolution analysis of the readthrough domain of beet necrotic yellow vein virus readthrough protein: a KTER motif is important for efficient transmission of the virus by Polymyxa betae

“…The recombinant plasmids used for production of in vitro run-off transcripts of wild-type BNYVV RNA 1 (pB15), RNA 2 (pB218 and pBF14), RNA 3 (pB35) and RNA 4 (pB45) have been described (Bouzoubaa el aL, 1986;Ziegler-Graff et al, 1988;Quillet et at., 1989). A plasmid (pB2-14) containing the complete cDNA of RNA 2 was obtained by introduction of the BstXI-SalI restriction fragment corresponding to residues 23354612 plus 3'-poly(A) tail [all sequence coordinates refer to the complete RNA 2 sequence (Bouzoubaa et al, 1986)] between the BstXI and Sall sites of pB218 (insert residues 1-2715).…”