Biologically active polypeptides in submandibular glands.

Barka, Tibor

doi:10.1177/28.8.7003006

Cited by 467 publications

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“…T he sexual dimorphism of the granular convoluted tubule (GCT) cells of the mouse submandibular gland (SMG) has been extensively documented by morphological studies at the light and electron microscopic levels and by the techniques of molecular biology, biochemistry, and immunocytochemistry (reviewed in Barka 1980;Gresik 1994). These studies show that the vast majority of the biologically active substances, such as epidermal growth factor (EGF), nerve growth factor (NGF), renin, tonin, and other proteinases, in addition to kallikrein gene family members (mK9, mK13, and mK22), are more abundant in the glands of males.…”

mentioning

confidence: 99%

Developmental and Androgenic Regulation of the Immunocytochemical Distribution of mK1, a True Tissue Kallikrein, in the Granular Convoluted Tubule of the Mouse Submandibular Gland

Kurabuchi

Hosoi

Gresik

2002

J Histochem Cytochem.

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S U M M A R YThe action of androgens on the immunocytochemical distribution of mK1, a true tissue kallikrein, was examined in the submandibular gland (SMG) of developing and adult mice by indirect enzyme-labeled and immunogold-labeled antibody methods for light and electron microscopy, respectively. In both sexes at 3 weeks of age, essentially all of the immature granular convoluted tubule (GCT) cells were uniformly immunostained. At 4 weeks of age (the onset of puberty), morphological differences between the two sexes appeared in the GCTs, in which some cells became immunonegative. Thereafter, the immunonegative GCT cells became more abundant in the SMG of males than of females and considerable intercellular variation in staining intensity for mK1 was seen, especially in males. A few slender GCT cells with strong immunoreactivity appeared in GCT segments only in males. Castration of males resulted in an increase in the number of immunopositive GCT cells, whereas administration of dihydrotestosterone (DHT) decreased the number of immunopositive GCT cells in the SMGs of both sexes. Slender GCT cells immunoreactive for mK1 were seen in females treated with DHT for 6 days. However, there were no immunostained slender GCT cells in female SMGs after injection of DHT for 2 weeks. Immunoelectron microscopy disclosed this type of cell in male SMGs, which closely resembles immature GCT cells of prepubertal mice, with a few small secretory granules uniformly labeled with gold particles, a sparse Golgi apparatus and RER, and basal infoldings. In mature male SMGs and in SMGs of DHT-treated females and castrated males, typical GCT cells had a well-developed Golgi apparatus and a net-like RER but few to no basal infoldings, whereas in the female gland equivalent cells had moderately developed RER and some basal infoldings. These results suggest that mK1 is one of the enzymes characteristically present in immature GCT cells and that its synthesis is inhibited in part by androgens, resulting in decreased numbers of immunopositive cells.

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confidence: 99%

Developmental and Androgenic Regulation of the Immunocytochemical Distribution of mK1, a True Tissue Kallikrein, in the Granular Convoluted Tubule of the Mouse Submandibular Gland

Kurabuchi

Hosoi

Gresik

2002

J Histochem Cytochem.

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“…The complexity of the present results is probable if one considers that the tremendous species differences, and quite often sex differences within species, occur with respect to the gross anatomy, histological architecture, histochemical staining, physiology, and biochemistry of the salivary gland duct systems (BARKA, 1980;PINKSTAFF, 1980). It was reported previously that proteinase isozyme patterns of the mouse SMG are different among various strains (HIRAMATSU et al, 1981).…”

Section: Discussionmentioning

confidence: 93%

“…The submandibular gland (SMG) of mice is known to have androgen receptors (VERHOEVEN and WILSON, 1976;VERHOEVEN, 1979), and to show prominent sex differences (BARKA, 1980;GRESIK, 1980;PINKSTAFF, 1980). Proteinases A and D (BoESMAN et al, 1976;SCHENKEIN et al, 1981), being identical to f3-nerve growth factor endopeptidase and epidermal growth factor binding protein, respectively (Hosoi et al, 1983a), and P-esterase (CALISSANO and ANGELETTI, 1968) are "androgen -inducible" proteinases (Hosol et al ., 1981) in this gland.…”

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confidence: 99%

Androgen responsiveness of proteinase F in the submandibular salivary glands of inbred mice.

et al. 1987

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Using the radioimmunoassay technique, we studied androgenic regulations of proteinase F in the submandibular salivary glands (SMG) of various mouse strains (ICR, BALB/CA, C3H/HeN, C57BL/ION, and DBA/2N). The proteinase contents in BALB/CA and DBA/2N mice were almost the same between males and females. However, prominent sex differences in enzyme content were seen in all other strains; i.e., male glands of C3H/HeN and C57BL/ l ON contained more proteinase F than female glands did. On the contrary, the enzyme level in the ICR was exceedingly higher in females than in males. The enzyme contents in ICR SMG increased in males following castration and decreased after androgen injection in both castrated males and normal females. On the contrary, the enzyme level in C3H/HeN was decreased by castration and increased by androgen injection. Therefore, these sex differences in ICR and C3H/HeN mice depend on androgen, though the direction of the response is opposite.

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“…Submandibular gland (SMG) is known to contain mK1, mK9, mK13, and mK26, including epidermal growth factor (EGF), nerve growth factor (NGF), and renin (Barka 1980). mK9, mK13, and mK26 are identical to EGF-binding protein (Taylor et al 1974), bNGF-endopeptidase (Smith et al 1968;Satch et al 1976Satch et al , 1980, and prorenin converting enzyme-2 (Kim et al 1991), respectively.…”

Section: Introductionmentioning

confidence: 99%

Hypophysectomy and hormonal therapy modulate mK1-immunoreactive duct cells in the mice sublingual glands

et al. 2008

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The immunocytochemical localization of a true tissue kallikrein, mK1, in mouse sublingual glands (SLGs) was examined following hypophysectomy and hormonal replacement therapy. In the glands of intact mice (14 weeks of age), mK1 was detected in the striated ducts (SDs). Full-fledged granular cells were scattered in the SDs of male mice (but not in those of female mice), showing a cellular mosaic distribution of mK1 with some being positive and others being negative. mK1 was also detected in transitional-type granular cells, though the secretory granules were too small and scarce to be visible by a light microscopy. Hypophysectomy in male mice resulted in the atrophy and loss of secretory granules in many SD cells. Granulation recovered after the repeated injection of 5a-dihydrotestosterone (DHT), 3,5,3 0 -triiodo-L thyronine (T 3 ), and dexamethasone (Dex), given either alone or in combination to the hypophysectomized mice. The concomitant injection of DHT and T 3 , with or without Dex, resulted in the reappearance of the full-fledged granular cells, only some of which were mK1-positive. Electron microscopy revealed mK1 to be present exclusively in the secretory granules of these mK1-positive cells, and no ultrastructural differences were observed between mK1-positive and mK1-negative full-fledged granular cells. These results show that the differentiation of the granular cell phenotype in the mouse SLG duct system requires the concomitant action of androgen and thyroid hormone and retards mK1 synthesis.

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Biologically active polypeptides in submandibular glands.

Cited by 467 publications

References 0 publications

Developmental and Androgenic Regulation of the Immunocytochemical Distribution of mK1, a True Tissue Kallikrein, in the Granular Convoluted Tubule of the Mouse Submandibular Gland

Developmental and Androgenic Regulation of the Immunocytochemical Distribution of mK1, a True Tissue Kallikrein, in the Granular Convoluted Tubule of the Mouse Submandibular Gland

Androgen responsiveness of proteinase F in the submandibular salivary glands of inbred mice.

Hypophysectomy and hormonal therapy modulate mK1-immunoreactive duct cells in the mice sublingual glands

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