Biological UV dosimetry—a comprehensive problem

Rontó, Gy.; Gróf, Pál; Gáspár, S.

doi:10.1016/1011-1344(95)07168-5

Cited by 33 publications

(13 citation statements)

References 16 publications

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“…In outdoor measurements, dosimeters were exposed to the sun either without amplification or for daily-profile measurements, using a specially developed collector system, as described earlier (17)(18)(19)). This collector system allows a 4OcI-fold amplification of the incident sunlight.…”

Section: Methodsmentioning

confidence: 99%

“…No difference in the spectral distribution was found in the spectra with and without the mirrors. Earlier measurements of the daily profile of solar radiation with phage T7 (18) showed good agreement between the measured dose-rate values of the daily profile and the calculated ones, based on model calculations (according to a modified Green's model) (25). On 8 June 1994 between 9:00 and 17:00, a simultaneous measurement of the cumulative dose of direct solar radiation with phage T7 and a uracil layer, in the latter case using a 400-fold amplification, was performed.…”

Section: Outdoor Measurements With Phage "7 and With Uracil Dosimetermentioning

confidence: 99%

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Use of Uracil Thin Layer for Measuring Biologically Effective UV Dose

Gróf

Gáspár

Rontó

1996

Photochem & Photobiology

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Dimerization of uracil monomers in a polycrystalline state by UV radiation changes the absorption characteristics of a thin layer of the material. The change in optical density, measured by spectrophotometry in the 250-400 nm range, as a function of the exposure time is evaluated in terms of the biologically effective UV dose. A statistical evaluation of a great number of uracil dosimeters irradiated with a TL01 lamp from Philips establishes the possibility of evaluating the biologically effective UV dose using a uracil dosimeter. Nonlinear regression procedures were introduced to correct the absorption spectra for contributions due to light scattering and to determine the optical density values required to calculate the UV dose expressed in HU units. Comparison of cumulative daily doses and long-term monitoring measured by the uracil thin-layer dosimeter and a phage T7 dosimeter are given, which allow the determination of conversion factors between various biological dosimeters under different irradiation conditions.

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Section: Methodsmentioning

confidence: 99%

Section: Outdoor Measurements With Phage "7 and With Uracil Dosimetermentioning

confidence: 99%

Use of Uracil Thin Layer for Measuring Biologically Effective UV Dose

Gróf

Gáspár

Rontó

1996

Photochem & Photobiology

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“…A recent application using a DNA repair-deficient Escherichia coli strain to measure UV in Antarctica (1 1-14) demonstrated sufficient sensitivity to detect UVB up to 25 m deep in Antarctic waters during the presence of an ozone "hole." Biological organisms, such as E. coli (11,15,16), bacteriophage TI, T2 and T7 (17)(18)(19), spores of Bacillus subtilis, both repair-deficient and wildtype strains (20)(21)(22)(23)(24), have some drawbacks in their application. First, different organisms with different repair capacities (12) confound comparisons.…”

Section: Introductionmentioning

confidence: 99%

“…In addition, repair-deficient mutants for all repair systems are largely unavailable in most organisms. Other materials for biologically effective dosimeters include uracil (19,26) and naked DNA from human fibroblasts (27). Because of the lower sensitivity in the detection of the formation of dimers in uracil by absorbance (280-290 nm), the uracil dosimeters are applicable to the long-term monitoring.…”

Section: Introductionmentioning

confidence: 99%

UVB DNA Dosimeters Analyzed by Polymerase Chain Reactions

Yoshida

Regan

1997

Photochem & Photobiology

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Purified bacteriophage lambda DNA was dried on a UV-transparent polymer film and served as a UVB dosimeter for personal and ecological applications. Bacteriophage lambda DNA was chosen because it is commercially available and inexpensive, and its entire sequence is known. Each dosimeter contained two sets of DNA sandwiched between UV-transparent polymer films, one exposed to solar radiation (experimental) and another protected from UV radiation by black paper (control). The DNA dosimeter was then analyzed by a polymerase chain reaction (PCR) that amplifies a 500 base pair specific region of lambda DNA. Photoinduced damage in DNA blocks polymerase from synthesizing a new strand; therefore, the amount of amplified product in UV-exposed DNA was reduced from that found in control DNA. The average lesion frequency per 500 base pair per strand at 16 PCR cycles was approximately 1.22, 1.00, 0.70 and 0.50 for 30 ng, 50 ng, 100 ng and 150 ng of dried DNA, respectively, after a total dose of 60 kJ m(-2) delivered with a solar UVB simulator. Although the average lesion frequency increases linearly with increasing doses for four different amounts of template DNA, the lesion frequency seems to be averaged by the amplified products from the protected lambda DNA molecules below the top few layers. The average daily dose, equivalent to the UV dose applied with the solar UVB simulator was 10.2 +/- 0.4 kJ m(-2) with the 50 ng containing DNA dosimeter in September 1995 in Melbourne, FL. Both 50 ng and 150 ng containing DNA dosimeters produced the same average daily dose within experimental error in January 1996, which was 5.2 +/- 0.3 kJ m(-2) at the same location. The dried lambda DNA dosimeter is compact, robust, safe and transportable, stable over long storage times and provides the total UVB dose integrated over the exposure time.

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“…Similar dimerization occurs in a polycrystalline uracil thin‐layer dosimeter, used in previous and present work as a biological dosimeter (29,30). Uracil dosimeter measures the biologically effective UV radiation with a spectral sensitivity very close to the DNA‐damaging spectra (30–32); thus, requirements (1), (3) and (4) are fulfilled. With the aim to provide information on UVB/UVA proportion also, an additional calculation will be presented.…”

Section: Introductionmentioning

confidence: 99%

Biological UV Dosimeters in Quality Control of Tanning Tubes¶

Kuluncsics¹,

Kerékgyártó²,

Gróf³

et al. 2007

Photochemistry and Photobiology

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Although according to the International Radiological Protection Association–International Non‐Ionizing Radiation Committee recommendation (1991) the use of sunbeds for cosmetic purposes is not recommended, tanning devices are used widely. Ten different types of commercially available sunbed tubes have been studied using a uracil biological UV dosimeter, and three of them were analyzed in detail. Dimerization effectiveness of the tubes was measured directly, whereas efficiency of erythema induction was calculated weighting the emission spectra by the Commission Internationale de l'Eclairage erythema action spectrum. The data obtained demonstrate that quality control of sunbed tubes has to include not only the determination of the UV doses administered but also the assessment of the health risk due to the UVB and UVA components of the lamp. A method of quality control using the uracil biological dosimeter was elaborated, and the estimation of the “acceptable” exposure time was checked/controlled on 15 volunteers by assessing individually the erythema induction threshold. A correct classification of the sunbed tubes is proposed by characterizing the erythema induction versus DNA‐damaging effectiveness of tubes.

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Biological UV dosimetry—a comprehensive problem

Cited by 33 publications

References 16 publications

Use of Uracil Thin Layer for Measuring Biologically Effective UV Dose

Use of Uracil Thin Layer for Measuring Biologically Effective UV Dose

UVB DNA Dosimeters Analyzed by Polymerase Chain Reactions

Biological UV Dosimeters in Quality Control of Tanning Tubes¶

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