Biological targets of neurotoxic pesticides analysed by alteration of developmental events in the Mediterranean sea urchin, Paracentrotus lividus

Pesando, D.; Huitorel, Philippe; Dolcini, Virginia; Angelini, Cristiano; Guidetti, Paolo; Falugi, Carla

doi:10.1016/s0141-1136(02)00215-5

Cited by 71 publications

(41 citation statements)

References 43 publications

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“…However, neurotoxins can affect neuro-transmitters such as AChE in organisms prior to the formation of the nervous system, and there is evidence to suggest they regulate developmental processes such as cell division and differentiation (Buznikov et al 2001). AChE is found in ascidian eggs and sperm (Pesando et al 2003) and in the eggs (Piomboni et al 2001) of sea urchins. The organophosphate diazinon decreases AChE activity and lectin binding in urchin eggs at 30 µg l -1 , resulting in a reduction in fertilisation (Pesando et al 2003).…”

Section: Discussionmentioning

confidence: 99%

“…AChE is found in ascidian eggs and sperm (Pesando et al 2003) and in the eggs (Piomboni et al 2001) of sea urchins. The organophosphate diazinon decreases AChE activity and lectin binding in urchin eggs at 30 µg l -1 , resulting in a reduction in fertilisation (Pesando et al 2003). If AChE is present in 3 h A. millepora embryos its function was not affected by chlorpyrifos exposures of up to 30 µg l -1 .…”

Section: Discussionmentioning

confidence: 99%

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Insecticides and a fungicide affect multiple coral life stages

Markey¹,

Baird²,

Humphrey³

et al. 2007

Mar. Ecol. Prog. Ser.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Insecticides and a fungicide affect multiple coral life stages

Markey¹,

Baird²,

Humphrey³

et al. 2007

Mar. Ecol. Prog. Ser.

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“…One of the most widely used organophosphate pesticides is diazinon. Diazinon is known to inhibit acetylcholinesterase, an enzyme responsible for inactivating the neurotransmitter acetylcholine in target and non-target organisms (Ecobichon & Joy, 1994;Pesando et al, 2003). Due to its chemical properties, widespread use and application, diazinon is frequently found in point sources (wastewater treatment plant effluent) and non-point sources (storm water runoff) in urban and agricultural areas, and known to be lethal to many aquatic species (EPA, 2003).…”

Section: Introductionmentioning

confidence: 99%

Effect of diazinon on life stages and resting egg hatchability of rotifer Brachionus plicatilis

Marcial

Hagiwara

2007

Hydrobiologia

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This paper has not been submitted elsewhere in identical or similar form, not will it be during the first three months after its submission to Hydrobiologia. showed that among the life history parameters, the time the rotifers took to release neonates is the most sensitive, giving the lowest EC 50 value of 1.24 mg/L. The fecundity of maternal females, amictic and mictic daughters was also investigated. Rotifers exposed to 10.0 mg/L produced significantly fewer amictic daughters, and at this concentration, rotifers did not produce any mictic daughter. At 5.0 mg/L, the number of male offspring was significantly lower than the control. Furthermore, the hatchability of resting eggs produced by the rotifers was evaluated when exposed to diazinon: from birth until they produced resting eggs (early development); during late developmental stage of resting eggs (before diapause); and during diapausing stage. The hatchability of the resting eggs was not affected when exposure was timed at late developmental and diapausing stages. Overall results showed that even though amictic females reproduced normally in the presence of low concentration of diazinon, sexual reproduction is severely affected, especially the hatchability of resting eggs when the exposure was timed on its early developmental stages. This provides another evidence that production of resting eggs is particularly sensitive to the presence of xenobiotics in the environment.

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“…Embryos are the most sensitive life stage of aquatic organisms and echinoid embryos (including sea urchins) have been used extensively as a valuable model embryonic system for studying the effects of environmental factors such as temperature, oxidative stress, chemical pollutants and UVR (Adams and Shick, 1996;Bancroft et al, 2007;Lesser et al, 2003;Pesando et al, 2003;Russo et al, 2003). Sea urchins inhabit all of Earth's major oceans and have life history stages sensitive to UVR (Adams and Shick, 1996;Anderson et al, 1993;Lesser et al, 2003).…”

Section: Introductionmentioning

confidence: 99%

Exposure to Ultraviolet Radiation Causes Proteomic Changes in Embryos of the Purple Sea Urchin, Strongylocentrotus Purpuratus

Campanale

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iv EXPOSURE TO ULTRAVIOLET RADIATION CAUSES PROTEOMIC CHANGES IN EMBRYOS OF THE PURPLE SEA URCHIN, STRONGYLOCENTROTUS PURPURATUS Joseph Paul CampanaleThe amount of solar ultraviolet radiation (UVR, 290-400 nm) reaching Earth's surface is increasing due to ozone depletion and global climate change. Embryos of the purple sea urchin, Strongylocentrotus purpuratus, provide an ideal system for examining how UVR affects developing marine organisms and cells in general. To model the protein-mediated cell cycle response to UV-irradiation, six batches of S. purpuratus embryos were exposed to UVR, monitored for delays in the first mitotic division and examined for global proteomic changes. Embryos from each batch were exposed to or protected from artificial UVR for 25 or 60 min. Embryos treated with UVR for 60 min cleaved an average of 23.24 min (±1.92 s.e.m) later than the UVprotected embryos. Protein expression of UV-protected and UV-treated embryos was examined at 30 and 90 min post-fertilization using two dimensional sodium dodecyl sulfate polyacrylamide gel electrophoresis (2D SDS-PAGE) and mass spectrometry (MS). Proteins were isoelectrically focused (pH 4-7) and separated by molecular weight using SDS-PAGE. At least 1,306 protein spots were detected in all gels. A total of 171 protein spots (13% of the detected proteome) migrated differently in UVtreated embryos at 30 min post-fertilization and 187 spots (14%) at 90 min postfertilization (2-way ANOVA, P= 0.03, n=6). Our results identify the differential migration of proteins from multiple cellular pathways and are the first to indicate that the mechanisms involved in the protein mediated UV-induced developmental delay are integrated among pathways for cellular stress, protein turnover and translation, signal transduction, general metabolism and involve the cytoskeleton.

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Biological targets of neurotoxic pesticides analysed by alteration of developmental events in the Mediterranean sea urchin, Paracentrotus lividus

Cited by 71 publications

References 43 publications

Insecticides and a fungicide affect multiple coral life stages

Insecticides and a fungicide affect multiple coral life stages

Effect of diazinon on life stages and resting egg hatchability of rotifer Brachionus plicatilis

Exposure to Ultraviolet Radiation Causes Proteomic Changes in Embryos of the Purple Sea Urchin, Strongylocentrotus Purpuratus

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