Biological synthesis of silver nanoparticles using β-1, 3 glucan binding protein and their antibacterial, antibiofilm and cytotoxic potential

Anjugam, Mahalingam; Vaseeharan, Baskaralingam; Iswarya, Arokiadhas; Divya, Mani; Prabhu, Nagesh; Sankaranarayanan, K.

doi:10.1016/j.micpath.2017.12.003

Cited by 57 publications

(20 citation statements)

References 53 publications

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“…• Phages reduced biofilm population 2 hr after the onset of infection • CT-PA treatment reduced biofilm biomass significantly, regardless of of antibiotic resistance or cystic fibrosis (CF) status (Fong, Drilling et al, 2017, Pires, Sillankorva, Faustino, & Azeredo, 2011 P. mirabilis vB_PmiS-TH • Combination of the phage with a high dose of ampicillin showed the highest biofilm removal activity after 24 hr (Yazdi, Bouzari, & Ghaemi, 2018) Mohammadi-Khorsand, & Shakibaie, 2015). Antibiofilm property of β1-3 glucan-binding protein-based silver nanoparticles (Ppβ-GBP-AgNPs) was assessed, and related findings demonstrated that it inhibited 85% and 80% of immature biofilms by E. faecalis and P. aeruginosa, respectively (Anjugam, Vaseeharan et al, 2018 (Comin, Lopes et al, 2016). On the other hand, green synthesized Nps (silver and gold), bimetallic (Ag/Au) Nps, and lectin-coated AgNPs reduced the biofilm formation and interfered with cell adhesion and polysaccharide matrix (Gopinath, Kumaraguru et al, 2016, Jayanthi, Shanthi et al, 2017.…”

Section: S Epidermidis Vb_sepis-phiipla5 and Vbsepis Phiipla7mentioning

confidence: 99%

Advanced strategies for combating bacterial biofilms

Sharahi

Azimi

Shariati

et al. 2019

Journal Cellular Physiology

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Biofilms are communities of microorganisms that are formed on and attached to living or nonliving surfaces and are surrounded by an extracellular polymeric material. Biofilm formation enjoys several advantages over the pathogens in the colonization process of medical devices and patients' organs. Unlike planktonic cells, biofilms have high intrinsic resistance to antibiotics and sanitizers, and overcoming them is a significant problematic challenge in the medical and food industries. There are no approved treatments to specifically target biofilms. Thus, it is required to study and present innovative and effective methods to combat a bacterial biofilm. In this review, several strategies have been discussed for combating bacterial biofilms to improve healthcare, food safety, and industrial process.

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Section: S Epidermidis Vb_sepis-phiipla5 and Vbsepis Phiipla7mentioning

confidence: 99%

Advanced strategies for combating bacterial biofilms

Sharahi

Azimi

Shariati

et al. 2019

Journal Cellular Physiology

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“…Crystal violet assay The effect of Pb-AgNPs on P. aeruginosa PAO1 biofilm formation was analyzed by the crystal violet (CV) assay in 96-MTP as performed by Anjugam et al (2018) with a few modifications. Briefly, an overnight grown and then diluted P. aeruginosa PAO1 culture in the absence and presence of increasing concentrations (0, 2, 4, 6, and 8 mg ml À1 ) of Pb-AgNPs was incubated for 18 h at 37 C. After this, planktonic cells were measured at OD 600 while the attached bacterial cells were washed with sterile phosphate buffer.…”

Section: Effect Of Pb-agnps On Pao1 Biofilmmentioning

confidence: 99%

“…Then the wells were washed with sterile DDW and the surface associated CV stain was solubilized with ethanol for another 30 min. The OD 570 was measured in an Epoch plate reader (Anjugam et al 2018). The percentage of PAO1 biofilm inhibition was calculated using the formula:…”

Section: Effect Of Pb-agnps On Pao1 Biofilmmentioning

confidence: 99%

Biofilm inhibition and anti-quorum sensing activity of phytosynthesized silver nanoparticles against the nosocomial pathogen Pseudomonas aeruginosa

et al. 2019

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Quorum sensing (QS), the communication signaling network, regulates biofilm formation and several virulence factors in Pseudomonas aeruginosa PAO1, a nosocomial opportunistic pathogen. QS is considered to be a challenging target for compounds antagonistic to virulent factors. Biologically synthesized silver nanoparticles (AgNPs) are reported as anti-QS and anti-biofilm drugs against bacterial infections. The present study reports on the synthesis and characterization of Piper betle (Pb) mediated AgNPs (Pb-AgNPs). The anti-QS activity of Pb-AgNPs against Chromobacterium violaceum and the potential effect of Pb-AgNPs on QS-regulated phenotypes in PAO1 were studied. FTIR analysis exhibited that Pb-AgNPs had been capped by phytochemical constituents of Pb. Eugenol is one of the active phenolic phytochemicals in Pb leaves, therefore molecular docking of eugenol-conjugated AgNPs on QS regulator proteins (LasR, LasI and MvfR) was performed. Eugenol-conjugated AgNPs showed considerable binding interactions with QS-associated proteins. These results provide novel insights into the development of phytochemically conjugated nanoparticles as promising anti-infective candidates.

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“…Therefore, there have been efforts toward the development of greener synthesis processes to produce Ag-NPs 11,12 with tailored properties that reduce toxicity against eukaryotic systems. 13 Although there are various methods to synthesize Ag-NPs, including chemical reduction, 14 photochemical reduction, 15,16 electrochemical reduction, 17 vaporization, and thermal synthesis, 18 there is a growing interest in developing a green synthesis method to produce nanoparticles using biological systems 19,20 and plant extracts. 21 Notwithstanding the diversity of green synthesis approaches, the use of plant extracts, as reductive media for nanoparticle synthesis, is one of the most convenient routes.…”

Section: Introductionmentioning

confidence: 99%

In vivo antimicrobial activity of silver nanoparticles produced via a green chemistry synthesis using <em>Acacia rigidula</em> as a reducing and capping agent

Escárcega‐González¹,

Garza-Cervantes²,

Vázquez-Rodríguez³

et al. 2018

IJN

135

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IntroductionOne of the main issues in the medical field and clinical practice is the development of novel and effective treatments against infections caused by antibiotic-resistant bacteria. One avenue that has been approached to develop effective antimicrobials is the use of silver nanoparticles (Ag-NPs), since they have been found to exhibit an efficient and wide spectrum of antimicrobial properties. Among the main drawbacks of using Ag-NPs are their potential cytotoxicity against eukaryotic cells and the latent environmental toxicity of their synthesis methods. Therefore, diverse green synthesis methods, which involve the use of environmentally friendly plant extracts as reductive and capping agents, have become attractive to synthesize Ag-NPs that exhibit antimicrobial effects against resistant bacteria at concentrations below toxicity thresholds for eukaryotic cells.PurposeIn this study, we report a green one-pot synthesis method that uses Acacia rigidula extract as a reducing and capping agent, to produce Ag-NPs with applications as therapeutic agents to treat infections in vivo.Materials and methodsThe Ag-NPs were characterized using transmission electron microscopy (TEM), high-resolution TEM, selected area electron diffraction, energy-dispersive spectroscopy, ultraviolet–visible, and Fourier transform infrared.ResultsWe show that Ag-NPs are spherical with a narrow size distribution. The Ag-NPs show antimicrobial activities in vitro against Gram-negative (Escherichia coli, Pseudomonas aeruginosa, and a clinical multidrug-resistant strain of P. aeruginosa) and Gram-positive (Bacillus subtilis) bacteria. Moreover, antimicrobial effects of the Ag-NPs, against a resistant P. aeruginosa clinical strain, were tested in a murine skin infection model. The results demonstrate that the Ag-NPs reported in this work are capable of eradicating pathogenic resistant bacteria in an infection in vivo. In addition, skin, liver, and kidney damage profiles were monitored in the murine infection model, and the results demonstrate that Ag-NPs can be used safely as therapeutic agents in animal models.ConclusionTogether, these results suggest the potential use of Ag-NPs, synthesized by green chemistry methods, as therapeutic agents against infections caused by resistant and nonresistant strains.

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Biological synthesis of silver nanoparticles using β-1, 3 glucan binding protein and their antibacterial, antibiofilm and cytotoxic potential

Cited by 57 publications

References 53 publications

Advanced strategies for combating bacterial biofilms

Advanced strategies for combating bacterial biofilms

Biofilm inhibition and anti-quorum sensing activity of phytosynthesized silver nanoparticles against the nosocomial pathogen Pseudomonas aeruginosa

In vivo antimicrobial activity of silver nanoparticles produced via a green chemistry synthesis using <em>Acacia rigidula</em> as a reducing and capping agent

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