Biological soft X-ray tomography on beamline 2.1 at the Advanced Light Source

Gros, Mark A. Le; McDermott, Gerry; Cinquin, Bertrand; Smith, Elizabeth A.; Do, Myan; Chao, Weilun; Naulleau, Patrick; Larabell, Carolyn A.

doi:10.1107/s1600577514015033

Cited by 87 publications

(80 citation statements)

References 28 publications

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“…They allow overpassing the limited accessibility of large facilities [3], maintaining a comparable spatial resolution [4]. Over the last few years many efforts have been made to perform nanometer spatial resolution imaging in the EUV and SXR spectral * corresponding author; e-mail: wachulak@gmail.com ranges, employing both large scale and compact sources.…”

Section: Introduction and Overview Of The Euv And Sxr Imaging Methodsmentioning

confidence: 99%

Nanoimaging Using Soft X-Ray and EUV Sources Based on Double Stream Gas Puff Targets

et al. 2018

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In this work we present recent results on nanoscale imaging in the extreme ultraviolet and soft X-ray spectral ranges, describing three novel imaging systems dedicated for high spatial resolution imaging of nanoscale objects with the extreme ultraviolet and soft X-ray radiations. The extreme ultraviolet and soft X-ray full field microscopes operate at 13.8 nm and 2.88 nm wavelengths and are capable of imaging of nanostructures with a sub-50 nm spatial resolution. A soft X-ray contact microscope operates in the "water-window" spectral range from 2.3 to 4.4 nm wavelength, to obtain images of an internal structure of the investigated object in a thin surface layer of soft X-ray light sensitive photoresist. The development of such compact imaging systems may, in the near future, be important from the point of view of new research related to biological, material science, and nanotechnology applications. Such preliminary applications are also shown in the studies of biological samples, including carcinoma cells, diatoms, and neurons.

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Section: Introduction and Overview Of The Euv And Sxr Imaging Methodsmentioning

confidence: 99%

Nanoimaging Using Soft X-Ray and EUV Sources Based on Double Stream Gas Puff Targets

et al. 2018

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“…It is the main reason why, until the development of compact sources, most of the studies in the field of EUV and SXR microscopy were conducted using synchrotron and free-electron laser facilities [4][5][6]. Such facilities are still being used not only for the purpose of high resolution imaging, but also for cutting-edge scientific experiments, providing the highest available photon flux, with the possibility to tune the generated wavelength and producing beams with adjustable spatial and temporal coherence.…”

Section: Introduction and Previous Achievementsmentioning

confidence: 99%

Bioimaging Using Full Field and Contact EUV and SXR Microscopes with Nanometer Spatial Resolution

et al. 2017

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We present our recent results, related to nanoscale imaging in the extreme ultraviolet (EUV) and soft X-ray (SXR) spectral ranges and demonstrate three novel imaging systems recently developed for the purpose of obtaining high spatial resolution images of nanoscale objects with the EUV and SXR radiations. All the systems are based on laser-plasma EUV and SXR sources, employing a double stream gas puff target. The EUV and SXR full field microscopes-operating at 13.8 nm and 2.88 nm wavelengths, respectively-are currently capable of imaging nanostructures with a sub-50 nm spatial resolution with relatively short (seconds) exposure times. The third system is a SXR contact microscope, operating in the "water-window" spectral range (2.3-4.4 nm wavelength), to produce an imprint of the internal structure of the investigated object in a thin surface layer of SXR light sensitive poly(methyl methacrylate) photoresist. The development of such compact imaging systems is essential to the new research related to biological science, material science, and nanotechnology applications in the near future. Applications of all the microscopes for studies of biological samples including carcinoma cells, diatoms, and neurons are presented. Details about the sources, the microscopes, as well as the imaging results for various objects will be shown and discussed.

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“…For this reason, until now, most of studies in this field are conducted using large scale facilities, such as synchrotron or free-electron laser installations [2][3][4] . Synchrotron and FEL facilities are being used for cutting-edge scientific experiments and provide highest available photon flux, tunability and spatial and temporal coherence.…”

Section: Introductionmentioning

confidence: 99%

Soft x-ray imaging with incoherent sources

Wachulak

Torrisi

Ayele

et al. 2017

X-Ray Lasers and Coherent X-Ray Sources: Development and Applications

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In this work we present experimental, compact desk-top SXR microscope, the EUV microscope which is at this stage a technology demonstrator, and finally, the SXR contact microscope. The systems are based on laser-plasma EUV and SXR sources, employing a double stream gas puff target. The EUV and SXR full field microscopes, operating at 13.8 nm and 2.88 nm wavelengths, respectively, are capable of imaging nanostructures with a sub-50 nm spatial resolution with relatively short (seconds) exposure times. The SXR contact microscope operates in the "water-window" spectral range, to produce an imprint of the internal structure of the sample in a thin layer of SXR light sensitive photoresist. Applications of such desk-top EUV and SXR microscopes for studies of variety of different samples -test objects for resolution assessment and other objects such as carbon membranes, DNA plasmid samples, organic and inorganic thin layers, diatoms, algae and carcinoma cells, are also presented. Details about the sources, the microscopes as well as the imaging results for various objects will be presented and discussed. The development of such compact imaging systems may be important to the new research related to biological, material science and nanotechnology applications.

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Biological soft X-ray tomography on beamline 2.1 at the Advanced Light Source

Cited by 87 publications

References 28 publications

Nanoimaging Using Soft X-Ray and EUV Sources Based on Double Stream Gas Puff Targets

Nanoimaging Using Soft X-Ray and EUV Sources Based on Double Stream Gas Puff Targets

Bioimaging Using Full Field and Contact EUV and SXR Microscopes with Nanometer Spatial Resolution

Soft x-ray imaging with incoherent sources

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