Biological Significance of Structural Adenovirus Components

Norrby, Erling

doi:10.1007/978-3-642-46118-7_1

Cited by 38 publications

(17 citation statements)

References 126 publications

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“…Favier et al predicted the theoretical PZC for each major capsid protein of HAdV 40 by using the primary sequences, and they showed that the PZC values for the hexon, penton base, long fiber protein, and short fiber protein were ϳ6.7, 5.8, 7.5, and 7.8, respectively (49). All of these values are higher than the HAdV 40 PZC we determined, which is consistent with prior observations that the PZC for an intact virion is lower than the PZC values for its structural protein components (54). PZC values were reported to be in the range of 3.…”

Section: Resultssupporting

confidence: 88%

Elution Is a Critical Step for Recovering Human Adenovirus 40 from Tap Water and Surface Water by Cross-Flow Ultrafiltration

Shi

Xagoraraki

Parent

et al. 2016

Appl Environ Microbiol

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This paper examines the recovery of the enteric adenovirus human adenovirus 40 (HAdV 40) by cross-flow ultrafiltration and interprets recovery values in terms of physicochemical interactions of virions during sample concentration. Prior to ultrafiltration, membranes were either blocked by exposure to calf serum (CS) or coated with a polyelectrolyte multilayer (PEM). HAdV 40 is a hydrophobic virus with a point of zero charge between pH 4.0 and pH 4.3. In accordance with predictions from the extended Derjaguin-Landau-Verwey-Overbeek theory, the preelution recovery of HAdV (r pre ) from deionized water was higher with PEMcoated membranes (r pre PEM ‫؍‬ 74.8% ؎ 9.7%) than with CS-blocked membranes (r pre CS ‫؍‬ 54.1% ؎ 6.2%). With either membrane type, the total virion recovery after elution (r post ) was high for both deionized water (r post PEM ‫؍‬ 99.5% ؎ 6.6% and r post CS ‫؍‬ 98.8% ؎ 7.7%) and tap water (r post PEM ‫؍‬ 89% ؎ 15% and r post CS ‫؍‬ 93.7% ؎ 6.9%). The nearly 100% recoveries suggest that the polyanion (sodium polyphosphate) and surfactant (Tween 80) in the eluent disrupt electrostatic and hydrophobic interactions between the virion and the membrane. Addition of EDTA to the eluent greatly improved the elution efficacy (r post CS ‫؍‬ 88.6% ؎ 4.3% and r post PEM ‫؍‬ 87.0% ؎ 6.9%) with surface water, even when the organic carbon concentration in the water was high (9.4 ؎ 0.1 mg/liter). EDTA likely disrupts cation bridging between virions and particles in the feed water matrix or the fouling layer on the membrane surface. For complex water matrices, the eluent composition is the most important factor for achieving high virion recovery. IMPORTANCEHerein we present the results of a comprehensive physicochemical characterization of HAdV 40, an important human pathogen. The data on HAdV 40 surface properties enabled rigorous modeling to gain an understanding of the energetics of virion-virion and virion-filter interactions. Cross-flow filtration for concentration and recovery of HAdV 40 was evaluated, with postelution recoveries from ultrapure water (99%), tap water (ϳ91%), and high-carbon-content surface water (ϳ84%) being demonstrated. These results are significant because of the very low adenovirus recoveries that have been reported, to date, for other methods. The recovery data were interpreted in terms of specific interactions, and the eluent composition was designed accordingly to maximize HAdV 40 recovery. P athogenic microorganisms cause many waterborne diseases, and the World Health Organization reports that water supply contamination leads to 842,000 deaths annually (1). Along with caliciviruses (e.g., norovirus), enteroviruses (e.g., poliovirus, coxsackievirus, and echovirus), and hepatitis A viruses, human adenovirus (HAdV) is one of the viral pathogens in EPA contaminant candidate lists 3 and 4 and is the second-leading cause of childhood gastroenteritis worldwide (2-4). Most cases of adenovirusassociated gastroenteritis stem from HAdV serotypes 40 and 41 (5), which are relatively resis...

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Section: Resultssupporting

confidence: 88%

Elution Is a Critical Step for Recovering Human Adenovirus 40 from Tap Water and Surface Water by Cross-Flow Ultrafiltration

Shi

Xagoraraki

Parent

et al. 2016

Appl Environ Microbiol

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“…It is of interest to analyze a possible relation of the observed cross-reactions to the sub groups of HAV in which the virus types show a subtle immunological interrelatedness [17], This also includes low-level cross-reactions. Of subgroup C, HAV 1, 2, and 5 were not neutralized by any of the SAV antisera, while HAV 6 of the same subgroup cross-reacted with four SAV antisera.…”

Section: Discussionmentioning

confidence: 99%

Immunological Relationship among Adenoviruses of Humans, Simians, and Nonprimates as Determined by the Neutralization Test

Willimzik¹,

Kalter²,

Lester³

et al. 1981

Intervirology

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98 antisera prepared against 25 simian adenoviruses and 31 adenovirus types from various nonprimate mammals and birds were tested for their ability to neutralize 35 human adenovirus prototypes. Antisera to these human adenovirus types were similarly tested against 25 simian adenovirus prototypes. Although very few reactions to the human adenoviruses were found in antisera to nonprimate viruses, cross-reactions among the simian and human adenoviruses were more frequent. The majority of these reactions, however, were of low titer and of questionable significance. Two chimpanzee adenovirus strains – Pan 9 and SAV 21 (C 1) – were found with an immunological relationship to human adenoviruses: Pan 9 strain showed a two-way cross relationship to human adenovirus 4, and SAV 21 (C 1) was neutralized by antisera prepared against human adenoviruses 14 and 16.

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“…Several review articles have been published, dealing with adenovirus components [2], with general [3,4] or selected [5] aspects of replica tion, with genetics [6], or with viral proteins [5,7].…”

mentioning

confidence: 99%

“…Properties of the two genera (M and A) are given separately, when distinct differences have been described. For more de tailed information, currently available reviews should be consulted [2][3][4][5][6][7] Aviadenovirus gal I (fowl adeno virus I).…”

mentioning

confidence: 99%