Biological significance of phosphoenolpyruvate carboxykinase in a cestode parasite,<i>Raillietina echinobothrida</i>and effect of phytoestrogens on the enzyme from the parasite and its host,<i>Gallus domesticus</i>

Ramnath,; Dutta, Asim Kumar; Dkhar, Barilin; Tandon, Veena; Das, Bidyadhar

doi:10.1017/s0031182017000518

Cited by 3 publications

(1 citation statement)

References 57 publications

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“…13 Then, a coupled spectrophotometric assay was reported, this time using pyruvate kinase (PK) and lactate dehydrogenase (LDH) with oxidation of NADH (Scheme 1c). 14,15 The methods presented in Scheme 1 were all developed between the 1950s and 1960s, but they are still in use for measuring the activity of PEPCK from different sources [16][17][18][19][20][21][22][23] with few or no modification. The two most used (Schemes 1b and 1c) rely on NADH-dependent enzymes in which the PEPCK activity is indirectly monitored by the absorbance decrease at 340 nm as a result of NADH depletion.…”

Section: Introductionmentioning

confidence: 99%

Direct Assay to Evaluate Phosphoenolpyruvate Carboxykinase Activity

Amaral

Correa

Corrêa

et al. 2019

J. Braz. Chem. Soc.

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Phosphoenolpyruvate carboxykinase (PEPCK) is a ubiquitous enzyme found in all known groups of organisms, acting in the reversible conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP) in the presence of divalent metal ion, and dependent of adenosine 5'-triphosphate (ATP) or guanosine-5'-triphosphate (GTP). PEPCK is an important enzyme in the metabolism of some organisms, such as Trypanosoma cruzi, being suggested as a potential drug target to treat Chagas' disease. Its catalytic activity is, classically, measured by coupled assays. Herein, a direct assay by liquid chromatography-tandem mass spectrometry (LC-MS/MS) capable of quantifying PEP, in co-elution with OAA by the differentiation obtained by the mass spectra, is reported. The developed assay was used throughout the purification protocol in order to measure the activity of PEPCK of T. cruzi, which was expressed in Escherichia coli. The purified enzyme was kinetically characterized by the developed method with Michaelis-Menten constant (K Mapp) values of 96 ± 4 and 275 ± 18 μmol L-1 to OAA and ATP as substrates, respectively. The developed assay was also used for ligand screening and proved to be able to identify very low inhibitions for small molecules (50 μmol L-1).

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Section: Introductionmentioning

confidence: 99%

Direct Assay to Evaluate Phosphoenolpyruvate Carboxykinase Activity

Amaral

Correa

Corrêa

et al. 2019

J. Braz. Chem. Soc.

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Genistein: is the multifarious botanical a natural anthelmintic too?

Tandon

Das

2018

J Parasit Dis

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Genistein (4',5,7-trihydroxyisoflavone) is naturally present in plants of the soy family and is known to have various pharmacological activities, such as anti-cancer, anti-diabetic, anti-oxidant, etc. The phytoestrogen is one of the major isoflavones found in some medicinal plants having anthelmintic properties. This review describes the putative role of genistein as an anthelmintic, which has been tested on some helminth parasites in vitro. Genistein has been shown to cause paralysis and alterations in the tegument and tegumental enzymes (acid phosphatase, alkaline phosphatase, adenosine triphosphatase, and 5'-nucleotidase) of helminth parasites. Alterations in the activities of several enzymes associated with the coordination system (specifically non-specific esterases, acetylcholine esterase, and nitric oxide synthase), and changes in the concentration of nitric oxide, cGMP, free amino acid pool, and tissue ammonia are observed in helminth parasites treated with genistein. The phytoestrogen also affects the carbohydrate metabolism by altering the activities of key enzymes involved in glycogen- and glucose-metabolism of a cestode parasite. Considering the significance of phosphoenolpyruvate carboxykinase (PEPCK) in glycolysis of the cestode parasite, i of the phytoestrogen for PEPCK in the parasite has been determined, and molecular docking of genistein into the active site of the enzyme has also been described. The potential beneficial role of genistein as a natural alternative in management of helminth parasites needs to be further explored, particularly considering its in vivo efficacy and pharmacokinetics.

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Comparative Proteomic Analysis of Different Parts of Taenia Hydatigena

Cai

et al. 2021

Front. Vet. Sci.

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Taenia hydatigena, a globally distributed parasite, is a canine tapeworm and causes huge economic losses in the food industry. Using LC-MS/MS, the proteomes of T. hydatigena cyst scolex, designated as CS, and the cyst without the scolex, designated as CWS, were profiled and a total of 764 different proteins were identified, 664 of which were identified in CS, 412 identified in CWS, and 312 in both. Comparative analysis revealed that CS had more abundant proteins associated with growth and development, while CWS had more abundant proteins constituting a scaffolding and protective extracellular matrix. Consistent with the sequencing data, the abundance of the five selected proteins was validated to be higher in CWS than CS by Western blotting. The current data will provide a clue for further pinpointing a role of these proteins in the biology of T. hydatigena.

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Biological significance of phosphoenolpyruvate carboxykinase in a cestode parasite,Raillietina echinobothridaand effect of phytoestrogens on the enzyme from the parasite and its host,Gallus domesticus

Cited by 3 publications

References 57 publications

Direct Assay to Evaluate Phosphoenolpyruvate Carboxykinase Activity

Direct Assay to Evaluate Phosphoenolpyruvate Carboxykinase Activity

Genistein: is the multifarious botanical a natural anthelmintic too?

Comparative Proteomic Analysis of Different Parts of Taenia Hydatigena

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