Biological Sciences and Analytical Chemistry. HPLC separation of ring D-conjugated estrogens.

Takanashi, Kaori; Watanabe, Kimitsuna; Itoh, Shinji; Kamada, Maki; Honma, T.; Yoshizawa, Itsuo

doi:10.2116/bunsekikagaku.44.793

Cited by 4 publications

(1 citation statement)

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“…The suppression voltage of the detector was fixed to 0.8 V vs the Hg/Hg 2 Cl 2 reference electrode, which was shown to be the most effective condition for measurement of E2 and its catechol derivatives (11). A reversed-phase column packed with TSK gel ODS-80T M (4.6 mm i.d.…”

Section: Hplcmentioning

confidence: 99%

Studies on the Interactions between Drugs and Estrogen: Analytical Method for Prediction System of Gynecomastia Induced by Drugs on the Inhibitory Metabolism of Estradiol Using Escherichia coli Coexpressing Human CYP3A4 with Human NADPH-Cytochrome P450 Reductase

Satoh

Fujita

Munakata

et al. 2000

Analytical Biochemistry

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Section: Hplcmentioning

confidence: 99%

Studies on the Interactions between Drugs and Estrogen: Analytical Method for Prediction System of Gynecomastia Induced by Drugs on the Inhibitory Metabolism of Estradiol Using Escherichia coli Coexpressing Human CYP3A4 with Human NADPH-Cytochrome P450 Reductase

Satoh

Fujita

Munakata

et al. 2000

Analytical Biochemistry

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Comparison of ex vivo inhibitory effect between 2‐hydroxyestradiol and its 17‐sulfate on rat hepatic microsomal lipid peroxidation

Takanashi¹,

Y²,

Kyo³

et al. 2003

Lipids

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Two endogenous antioxidants that are speculated to be defense substances against preeclampsia, 2-hydroxyestradiol (2-OH-E2) and its 17-sulfate, 2-hydroxyestradiol 17-sulfate (2-OH-E2-17-S), were administered to rats to compare their inhibitory effects on hepatic microsomal lipid peroxidation, and the lipid peroxides were determined in NADPH- and ascorbic acid-dependent systems. The two catechols showed a strong inhibitory effect on lipid peroxidation in both systems, and the effect was dose dependent. However, a large difference was observed in their inhibition patterns. After administration of 2-OH-E2, the effect appeared immediately and decreased gradually with time. In contrast, the effect of 2-OH-E2-17-S appeared some time after administration and persisted for a longer time. Both catechols also showed a striking difference in their dynamics. After administration, 2-OH-E2 was detected in the blood together with its metabolites, 2-methoxyestradiol and 2-methoxyestrone, and they disappeared immediately. In contrast, 2-OH-E2-17-S was present in the blood for a longer time together with its O-methylated product, 2-methoxyestradiol 17-sulfate, but disappeared from liver microsomes within 2 h after administration. The results imply no occurrence of a direct inhibition effect of 2-OH-E2-17-S.

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Gas chromatography and high-performance liquid chromatography of natural steroids

Shimada¹,

Mitamura²,

Higashi³

2001

Journal of Chromatography A

171

106

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Biological Sciences and Analytical Chemistry. HPLC separation of ring D-conjugated estrogens.

Cited by 4 publications

References 0 publications

Studies on the Interactions between Drugs and Estrogen: Analytical Method for Prediction System of Gynecomastia Induced by Drugs on the Inhibitory Metabolism of Estradiol Using Escherichia coli Coexpressing Human CYP3A4 with Human NADPH-Cytochrome P450 Reductase

Studies on the Interactions between Drugs and Estrogen: Analytical Method for Prediction System of Gynecomastia Induced by Drugs on the Inhibitory Metabolism of Estradiol Using Escherichia coli Coexpressing Human CYP3A4 with Human NADPH-Cytochrome P450 Reductase

Comparison of ex vivo inhibitory effect between 2‐hydroxyestradiol and its 17‐sulfate on rat hepatic microsomal lipid peroxidation

Gas chromatography and high-performance liquid chromatography of natural steroids

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