Biological role of MicroRNA-103 based on expression profile and target genes analysis in pigs

Li, Guoxi; Wu, Zongsong; Li, Xinjian; Ning, Xiaomin; Li, Yanjie; Yang, Gongshe

doi:10.1007/s11033-010-0615-z

Cited by 26 publications

(23 citation statements)

References 42 publications

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“…As shown in Supplementary Fig. 2, the expression of the PPARg targets miR-103 and miR-107, which have previously been demonstrated to be induced during differentiation, was up-regulated in Lkb1-shRNA-expressing cells, particularly in the late stages of differentiation (day 6; Esau et al 2004, Li et al 2011. Interestingly, the expression of Cebpd was significantly higher in Lkb1-shRNA-expressing cells 30 min after the induction of differentiation (Fig.…”

Section: Lkb1 Depletion Facilitates 3t3-l1 Adipocyte Differentiationsupporting

confidence: 52%

LKB1 signalling attenuates early events of adipogenesis and responds to adipogenic cues

Gormand¹,

Berggreen²,

Amar³

et al. 2014

Journal of Molecular Endocrinology

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cAMP-response element-binding protein (CREB) is required for the induction of adipogenic transcription factors such as CCAAT/enhancer-binding proteins (C/EBPs). Interestingly, it is known from studies in other tissues that LKB1 and its substrates AMP-activated protein kinase (AMPK) and salt-inducible kinases (SIKs) negatively regulate gene expression by phosphorylating the CREB co-activator CRTC2 and class IIa histone deacetylases (HDACs), which results in their exclusion from the nucleus where they co-activate or inhibit their targets. In this study, we show that AMPK/SIK signalling is acutely attenuated during adipogenic differentiation of 3T3-L1 preadipocytes, which coincides with the dephosphorylation and nuclear translocation of CRTC2 and HDAC4. When subjected to differentiation, 3T3-L1 preadipocytes in which the expression of LKB1 was stably reduced using shRNA (Lkb1-shRNA), as well as Lkb1-knockout mouse embryonic fibroblasts (Lkb1 K/K MEFs), differentiated more readily into adipocyte-like cells and accumulated more triglycerides compared with scrambled-shRNA-expressing 3T3-L1 cells or Wt MEFs. In addition, the phosphorylation of CRTC2 and HDAC4 was reduced, and the mRNA expression of adipogenic transcription factors Cebpa, peroxisome proliferator-activated receptor g (Pparg) and adipocyte-specific proteins such as hormone-sensitive lipase (HSL), fatty acid synthase (FAS), aP2, GLUT4 and adiponectin was increased in the absence of LKB1. The mRNA and protein expression of Ddit3/CHOP10, a dominant-negative member of the C/EBP family, was reduced in Lkb1-shRNA-expressing cells, providing a potential mechanism for the up-regulation of Pparg and Cebpa expression. These results support the hypothesis that LKB1 signalling keeps preadipocytes in their non-differentiated form.

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Section: Lkb1 Depletion Facilitates 3t3-l1 Adipocyte Differentiationsupporting

confidence: 52%

LKB1 signalling attenuates early events of adipogenesis and responds to adipogenic cues

Gormand¹,

Berggreen²,

Amar³

et al. 2014

Journal of Molecular Endocrinology

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show abstract

“…For fat traits, miR-103 and miR-107 were highly correlated (Table 3). This is consistent with previous reports of miR-103 being involved in adipogenesis, lipid metabolism, and adipocyte differentiation [54,55] and of miR-103/107 being involved in glucose homeostasis and insulin sensitivity [56]. …”

Section: Discussionsupporting

confidence: 93%

Correlated mRNAs and miRNAs from co-expression and regulatory networks affect porcine muscle and finally meat properties

Ponsuksili

Hadlich

et al. 2013

BMC Genomics

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BackgroundPhysiological processes aiding the conversion of muscle to meat involve many genes associated with muscle structure and metabolic processes. MicroRNAs regulate networks of genes to orchestrate cellular functions, in turn regulating phenotypes.ResultsWe applied weighted gene co-expression network analysis to identify co-expression modules that correlated to meat quality phenotypes and were highly enriched for genes involved in glucose metabolism, response to wounding, mitochondrial ribosome, mitochondrion, and extracellular matrix. Negative correlation of miRNA with mRNA and target prediction were used to select transcripts out of the modules of trait-associated mRNAs to further identify those genes that are correlated with post mortem traits.ConclusionsPorcine muscle co-expression transcript networks that correlated to post mortem traits were identified. The integration of miRNA and mRNA expression analyses, as well as network analysis, enabled us to interpret the differentially-regulated genes from a systems perspective. Linking co-expression networks of transcripts and hierarchically organized pairs of miRNAs and mRNAs to meat properties yields new insight into several biological pathways underlying phenotype differences. These pathways may also be diagnostic for many myopathies, which are accompanied by deficient nutrient and oxygen supply of muscle fibers.

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“…Previous studies demonstrated that miR-103 was involved in various biological processis such as brain development, adipocyte differentiation, lipid metabolism, hematopoiesis, and immunity [55]–[58]. A recent study has reported that miR-103 also expressed in testis of piglets but not in adult pig testis, which indicates that miR-103 expression pattern depends on the developmental stages of pigs and may have important biological roles in testis [8]. miR-202-5p was highly expressed in adult human testis, which is consistent with the observation in mouse testis [6].…”

Section: Discussionmentioning

confidence: 97%

MicroRNA and piRNA Profiles in Normal Human Testis Detected by Next Generation Sequencing

et al. 2013

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BackgroundMicroRNAs (miRNAs) are the class of small endogenous RNAs that play an important regulatory role in cells by negatively affecting gene expression at transcriptional and post-transcriptional levels. There have been extensive studies aiming to discover miRNAs and to analyze their functions in the cells from a variety of species. However, there are no published studies of miRNA profiles in human testis using next generation sequencing (NGS) technology.ResultsWe employed Solexa sequencing technology to profile miRNAs in normal human testis. Total 770 known and 5 novel human miRNAs, and 20121 piRNAs were detected, indicating that the human testis has a complex population of small RNAs. The expression of 15 known and 5 novel detected miRNAs was validated by qRT-PCR. We have also predicted the potential target genes of the abundant known and novel miRNAs, and subjected them to GO and pathway analysis, revealing the involvement of miRNAs in many important biological phenomenon including meiosis and p53-related pathways that are implicated in the regulation of spermatogenesis.ConclusionsThis study reports the first genome-wide miRNA profiles in human testis using a NGS approach. The presence of large number of miRNAs and the nature of their target genes suggested that miRNAs play important roles in spermatogenesis. Here we provide a useful resource for further elucidation of the regulatory role of miRNAs and piRNAs in the spermatogenesis. It may also facilitate the development of prophylactic strategies for male infertility.

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Biological role of MicroRNA-103 based on expression profile and target genes analysis in pigs

Cited by 26 publications

References 42 publications

LKB1 signalling attenuates early events of adipogenesis and responds to adipogenic cues

LKB1 signalling attenuates early events of adipogenesis and responds to adipogenic cues

Correlated mRNAs and miRNAs from co-expression and regulatory networks affect porcine muscle and finally meat properties

MicroRNA and piRNA Profiles in Normal Human Testis Detected by Next Generation Sequencing

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