Biological responses of ligament fibroblasts and gene expression profiling on micropatterned silicone substrates subjected to mechanical stimuli

Park, Su A; Kim, In Ae; Lee, Yong Jae; Shin, Ji Won; Kim, Chong-Rak; Kim, Jeong Koo; Yang, Young‐Il; Shin, Jung-Woog

doi:10.1263/jbb.102.402

Cited by 54 publications

(40 citation statements)

References 29 publications

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“…We examined the morphological effects of a wide range of mechanical forces within the physiological and non-physiological regimes and found mechanical force magnitude and direction to influence the actin cytoskeleton reorientation of NIH 3T3 fibroblasts and HUVECs in isolation. Although these cells are primarily contact inhibited in vivo, previous studies show both fibroblasts and endothelial cells under mechanical stimulation adopt the same orientation in isolation (Neidlinger-Wilke et al, 2002;WojciakStothard and Ridley, 2003) and in monolayers (Malek and Izumo, 1996;Park et al, 2006), which suggests our results could be true regardless of whether contact inhibition occurs or not. Nonetheless, it would be interesting to compare the effects of dual integrated force inputs on single cells and cellular monolayers using metrics beyond those used in those study such as cellular contractility and cell stiffness, for example.…”

Section: Discussionsupporting

confidence: 55%

Cellular force signal integration through vector logic gates

Steward

Tan

Cheng

et al. 2015

Journal of Biomechanics

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Section: Discussionsupporting

confidence: 55%

Cellular force signal integration through vector logic gates

Steward

Tan

Cheng

et al. 2015

Journal of Biomechanics

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“…Our approach could not only impose mechanical stretching, but also impart fluid shear stress on cells. Most studies involving mechanical forces on fibroblasts generally involve tensile forces via cyclic or static loading modes [18][19][20][33][34][35]. Recent studies, however, have also revealed the influence of shear stress on fibroblast behavior [13].…”

Section: Resultsmentioning

confidence: 99%

Probing Cell Structure Responses Through a Shear and Stretching Mechanical Stimulation Technique

Steward

Cheng

Wang

et al. 2009

Cell Biochem Biophys

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Cells are complex, dynamic systems that respond to various in vivo stimuli including chemical, mechanical, and scaffolding alterations. The influence of mechanics on cells is especially important in physiological areas that dictate what modes of mechanics exist. Complex, multivariate physiological responses can result from multi-factorial, multi-mode mechanics, including tension, compression, or shear stresses. In this study, we present a novel device based on elastomeric materials that allowed us to stimulate NIH 3T3 fibroblasts through uniaxial strip stretching or shear fluid flow. Cell shape and structural response was observed using conventional approaches such as fluorescent microscopy. Cell orientation and actin cytoskeleton alignment along the direction of applied force were observed to occur after an initial 3 h time period for shear fluid flow and static uniaxial strip stretching experiments although these two directions of alignment were oriented orthogonal relative to each other. This response was then followed by an increasingly pronounced cell and actin cytoskeleton alignment parallel to the direction of force after 6, 12, and 24 h, with 85% of the cells aligned along the direction of force after 24 h. These results indicate that our novel device could be implemented to study the effects of multiple modes of mechanical stimulation on living cells while probing their structural response especially with respect to competing directions of alignment and orientation under these different modes of mechanical stimulation. We believe that this will be important in a diversity of fields including cell mechanotransduction, cell-material interactions, biophysics, and tissue engineering.

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“…After 6 and 24 h, increased proliferation was observed following a period of 15 and 60 min of stretching. Furthermore, there have been some reports where an appropriate level of mechanical force was shown to stimulate or inhibit cell proliferation [48][49][50][51][52][53][54][55]. Kaspar et al [56] reported an increase in human osteoblastic cell proliferation and type I collagen propeptide release under mechanical strain (1,000 μ strain, 1 Hz, 30 min/day, for 2 days).…”

Section: Discussionmentioning

confidence: 99%

Effect of mechanical tension on the human dental pulp cells

Han

Seo

Yoon

et al. 2008

Biotechnol Bioproc E

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In this study, we have evaluated the effects of mechanical tension on the proliferation and extracellular matrix (ECM) production of human dental pulp stem cells (DPSCs) using a flexwell system that imposed cyclic mechanical tension at 0.03 Hz with 0, 5, and 8% strains. In the early stage (4 days), DPSCs at 5 and 8% strains had a similar proliferation, which was higher than the control. However, in the late stage (10 days), DPSCs at 8% strain had a higher proliferation than the control and 5% strains. This result clearly demonstrated that DPSC proliferation under tension varied with culture time. In addition, mechanical tension was shown to increase the amount of lactate dehydrogenase (LDH) released during culture. RT-PCR analysis was used to show that mechanical tension also increased collagen and osteopontin expression and decreased α-smooth muscle actin (α-SMA) expression. Furthermore, FACS analysis showed that CD105 expression did not change in all groups but CD 90 expression decreased at 8% strain. In conclusion, our results suggest that an appropriate level of mechanical tension can serve as a potent positive modulator of proliferation, differentiation and ECM production in DPSCs. © KSBB hÉóïçêÇëW=ãÉÅÜ~åáÅ~ä=íÉåëáçåI=éêçäáÑÉê~íáçåI=ÇÉåí~ä=éìäé=ÅÉää= = = = =

show abstract

Biological responses of ligament fibroblasts and gene expression profiling on micropatterned silicone substrates subjected to mechanical stimuli

Cited by 54 publications

References 29 publications

Cellular force signal integration through vector logic gates

Cellular force signal integration through vector logic gates

Probing Cell Structure Responses Through a Shear and Stretching Mechanical Stimulation Technique

Effect of mechanical tension on the human dental pulp cells

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