Biological response of HeLa cells to gold nanoparticles coated with organic molecules

Cardoso-Ávila, Pablo Eduardo; Mendoza, A; Pichardo‐Molina, J. L.; Villavicencio, Lérida Liss Flores; Domínguez, José Pedro Castruita; C, Murali Krishna; López, Myrna Sabanero

doi:10.1016/j.tiv.2017.04.013

Cited by 15 publications

(5 citation statements)

References 42 publications

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“…We found that only 1D-AuNPs and 1D-AuNPs-FA and 2D-AuNPs showed significant change through all concentrations. Journal of Nanomaterials gold NPs coated with 4-ATP and BSA [27]. Our results obtained from XTT assays are not conclusive at all, but they still offer important information on the toxicity of these NPs, suggesting that more tests need to be carried out to get more information related to the possible damage effects of gold NPs and the coatings we used here.…”

Section: Biological Response Of Hela and Sh-sy5y Cells Tomentioning

confidence: 67%

“…While some reports affirm to have observed convincing toxicological results, others show that they are entirely biocompatible. Since it is undeniable that NPs are part of our day-to-day, more in vitro studies can be helpful to evaluate and determine their toxic effects [23][24][25][26][27][28] before they can be evaluated in animal models or moved on to testing in human beings [29].…”

Section: Introductionmentioning

confidence: 99%

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Biological Impact of the Interaction of Functionalized and Bioconjugated Gold Nanoparticles of Different Sizes on HeLa and SH‐SY5Y Human Cell Lines

Pichardo‐Molina

Andrade-Pérez

Torres-Ramírez

et al. 2022

Journal of Nanomaterials

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Nanotechnology opens new perspectives in many science and technology fields through new materials, such as metal nanoparticles. The biomedical field is one of these areas where nanoparticles offer promising application in the diagnosis and therapy of disease, generating biosensors for disease detection, bioimaging, and drug delivery. However, the controversy about whether nanoparticles are inert or exhibit different degrees of toxicity related to their physicochemical properties remains a subject of study and debate. This work shows how gold nanoparticles and nanorods were simultaneously functionalized with 4-aminothiophenol (4-ATP) and methoxypolyethylene glycol thiol (mPEG-SH). Then, folic acid (FA) gold nanoparticle bioconjugates caused diverse biological effects on HeLa and SH-SY5Y cell cultures after 24 h of incubation, when they were evaluated in the range of gold concentrations from 0.17 to 350 μM. We found notable changes in cell metabolic activity, viability, and biomass. 16 nm nanoparticles produced the most enhanced damage for functionalized (AuNPs) and bioconjugated (AuNPs-FA) nanoparticles, which we expected to be more biocompatible when coated with FA. Besides, epifluorescence images showed damage to F-actin microfilaments, adhesion and cell attachment loss, morphology changes (cells became round and detached), presence of blebs, and rupture of cell membrane. These results suggested that both AuNPs and AuNPs-FA have significant biological impact on HeLa and SH-SY5Y cell cultures, whereas gold nanorods showed significant changes only in the metabolic activity of SH-SY5Y cells when they were exposed to gold nanorods of 23 and 27 nm in length with a diameter of 5 nm, and crystal violet did not show evidence of toxicity.

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Section: Biological Response Of Hela and Sh-sy5y Cells Tomentioning

confidence: 67%

Section: Introductionmentioning

confidence: 99%

Biological Impact of the Interaction of Functionalized and Bioconjugated Gold Nanoparticles of Different Sizes on HeLa and SH‐SY5Y Human Cell Lines

Pichardo‐Molina

Andrade-Pérez

Torres-Ramírez

et al. 2022

Journal of Nanomaterials

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“… 38,39 Avila et al have also reported the preparation of AuNPs co-coated with both cysteamine and BSA, by mixing cysAuNPs with BSA. 40 The high binding affinity of BSA towards metal surfaces could be attributed to its high-molecular-weight charges, as well as the prevalence of carboxylate, thiol, amine, and imidazole groups within the protein structure. 39,41–43 Despite that, the positive error of BSA could be easily identified and eliminated based on its outlier dispersion ratios in both modes.…”

Section: Resultsmentioning

confidence: 99%

Cysteamine-coated gold nanoparticles for bimodal colorimetric detection with inverse sensitivity: a proof-of-concept with lysozyme

Wai

New

2020

RSC Adv.

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“…The investigation of ASCs after AuNPs uptake on a tridimensional polyethylene glycol modified fibrin gel system has allowed a long-term cell tracking with ultrasound and photoacoustic imaging, and observed no negative effect on cell morphology, viability, proliferation, network formation, and on expression and secretion of angiogenesis-related proteins and pericyte markers; maintaining the ASCs paracrine effect [ 50 ]. Gold nanoparticles have been used in tracking AuNPs labeled cells transplanted into animal models, using ultrasound and photoacoustic imaging [ 10 , 13 , 51 ], also for real time guided cell delivery [ 13 ], and Micro-Computed Tomography [ 11 , 12 , 49 ].…”

Section: Discussionmentioning

confidence: 99%

“…Gold nanoparticles (AuNPs) have numerous biomedical applications [ 1 ]; among diagnostics [ 2 ], cancer therapeutic use [ 2 , 3 , 4 , 5 , 6 , 7 ], enhancement in radiological imaging [ 8 ], improvement of transplantable matrices properties [ 9 ], as biotracers [ 3 , 10 , 11 , 12 , 13 ], for drug delivery [ 3 , 14 ], etc. Even though Nano Particles (NPs) can be prepared from metal and non-metal polymers, bioceramics, etc., the detection methods for metallic NPs could facilitate their observation with non-invasive detection methods, using tracing agents with high resolution and longer detection time, without intensity attenuation [ 15 ].…”

Section: Introductionmentioning

confidence: 99%

Laser Ablated Albumin Functionalized Spherical Gold Nanoparticles Indicated for Stem Cell Tracking

Dziedzic¹,

Mogharbel²,

Irioda³

et al. 2023

Materials

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Cell tracking in cell-based therapy applications helps distinguish cell participation among paracrine effect, neovascularization, and matrix deposition. This preliminary study examined the cellular uptake of gold nanoparticles (AuNPs), observing cytotoxicity and uptake of different sizes and AuNPs concentrations in Adipose-derived stromal cells (ASCs). ASCs were incubated for 24 h with Laser ablated Albumin functionalized spherical AuNPs (LA-AuNPs), with average sizes of 2 nm and 53 nm in diameter, in four concentrations, 127 µM, 84 µM, 42 µM, and 23 µM. Cytotoxicity was examined by Live/Dead assay, and erythrocyte hemolysis, and the effect on the cytoskeleton was investigated by immunocytochemistry for β-actin. The LA-AuNPs were internalized by the ASCs in a size and concentration-dependent manner. Clusters were observed as dispersed small ones in the cytosol, and as a sizeable perinuclear cluster, without significant harmful effects on the cells for up to 2 weeks. The Live/Dead and hemolysis percentage results complemented the observations that the larger 53 nm LA-AuNPs in the highest concentrated solution significantly lowered cell viability. The demonstrated safety, cellular uptake, and labelling persistency with LA-AuNPs, synthesized without the combination of chemical solutions, support their use for cell tracking in tissue engineering applications.

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Biological response of HeLa cells to gold nanoparticles coated with organic molecules

Cited by 15 publications

References 42 publications

Biological Impact of the Interaction of Functionalized and Bioconjugated Gold Nanoparticles of Different Sizes on HeLa and SH‐SY5Y Human Cell Lines

Biological Impact of the Interaction of Functionalized and Bioconjugated Gold Nanoparticles of Different Sizes on HeLa and SH‐SY5Y Human Cell Lines

Cysteamine-coated gold nanoparticles for bimodal colorimetric detection with inverse sensitivity: a proof-of-concept with lysozyme

Laser Ablated Albumin Functionalized Spherical Gold Nanoparticles Indicated for Stem Cell Tracking

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