Biological recovery and properties of poly(3-hydroxybutyrate) from Cupriavidus necator H16

Kunasundari, Balakrishnan; Arza, Carlos Rodriquez; Maurer, Frans H.J.; Murugaiyah, Vikneswaran; Kaur, Gurjeet; Sudesh, Kumar

doi:10.1016/j.seppur.2016.07.043

Cited by 38 publications

(16 citation statements)

References 22 publications

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“…When the lytic cycle is completed, the virus escapes from the host cell by breaking down the cell wall; this breaking down also liberates the PHA particles allowing their recovery [ 162 ]. In recent years, biological disruption methods included bacteria predators, rats, and mealworms [ 178 , 179 , 180 ].…”

Section: Pha Recoverymentioning

confidence: 99%

Recent Advances and Challenges towards Sustainable Polyhydroxyalkanoate (PHA) Production

et al. 2017

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Sustainable biofuels, biomaterials, and fine chemicals production is a critical matter that research teams around the globe are focusing on nowadays. Polyhydroxyalkanoates represent one of the biomaterials of the future due to their physicochemical properties, biodegradability, and biocompatibility. Designing efficient and economic bioprocesses, combined with the respective social and environmental benefits, has brought together scientists from different backgrounds highlighting the multidisciplinary character of such a venture. In the current review, challenges and opportunities regarding polyhydroxyalkanoate production are presented and discussed, covering key steps of their overall production process by applying pure and mixed culture biotechnology, from raw bioprocess development to downstream processing.

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Section: Pha Recoverymentioning

confidence: 99%

Recent Advances and Challenges towards Sustainable Polyhydroxyalkanoate (PHA) Production

et al. 2017

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“…Commercial PHAs are nowadays produced through fermentation of glucose or agricultural sugar substrates, which account for 30-40% of the total production costs [13][14][15]. To date, CH 4 -based biopolymer production has been focused on the synthesis of poly-3-hydroxybutyrate (PHB), which presents similar mechanical and thermal characteristics to those of conventional plastics and is biodegradable, thus enabling its rapid decomposition in the environment [16,17]. Recent attention has been paid to the optimization of PHB accumulation from a microbiological point of view by identifying the key limiting macro/micronutrients that boost PHB synthesis in methanotrophs.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous methane abatement and PHB production by Methylocystis hirsuta in a novel gas-recycling bubble column bioreactor

García-Pérez

López

Passos

et al. 2018

Chemical Engineering Journal

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The limited gas-liquid mass transfer represents the main challenge in the operation of costeffective bioreactors devoted to the treatment of poorly soluble gas pollutants such as methane (CH 4). This study evaluates the influence of internal gas-recycling strategies on the enhancement of CH 4 abatement in a bubble column bioreactor inoculated with the methanotroph Methylocystis hirsuta. Maximum CH 4 removal efficiencies of 72.9 ± 0.5 % (corresponding to elimination capacities of 35.2 ± 0.4 g m-3 h-1) were recorded under process operation at an empty bed residence time of 30 min and 0.50 m 3 gas m-3 reactor min-1 of internal gas-recycling rate. The accumulation of poly-3-hydroxybutyrate (PHB) in M. hirsuta was evaluated batchwise under limitations of potassium, manganese, nitrogen, and nitrogen with excess of iron. Nitrogen starvation resulted in the highest PHB content (28 ± 1 %). Likewise, the implementation of sequential N starvation cycles in a continuous bubble column reactor operated at a gas residence time of 30 min and an internal gasrecycling rate of 0.50 m 3 gas m-3 reactor min-1 supported a PHB content of up to 34.6 ± 2.5 %, with a volumetric PHB productivity of 1.4 ± 0.4 kg m-3 d-1 and elimination capacities of 16.2 ± 9.5 g m-3 h-1 .

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“…Mealworm has been recently reported for their ability to degrade polystyrene (PS) and two PS‐degrading bacterial strains were isolated from the mealworm gut . The remarkable findings of of the ability of mealworm in extracting PHAs granules has led to this study. Mealworms are omnivorous insects that can grow on low‐quality feeds such as organic wastes .…”

Section: Discussionmentioning

confidence: 99%

Production and recovery of poly(3‐hydroxybutyrate‐co‐3‐hydroxyvalerate) from biodiesel liquid waste (BLW)

2018

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Polyhydroxyalkanoates (PHAs) has been paid great attention because of its useful thermoplastic properties and complete degradation in various natural environments. But, at industrial level, the successful commercialization of PHAs is limited by the high production cost due to the expensive carbon source and recovery processes. Pseudomonas mendocina PSU cultured for 72 h in mineral salts medium (MSM) containing 2% (v/v) biodiesel liquid waste (BLW) produced 79.7 wt% poly(3-hydroxybutyrate) (PHB) at 72 h. In addition, this strain produced 43.6 wt% poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) with 8.6 HV mol% at 60 h when added with 0.3% sodium propionate. The synthesized intracellular PHA granules were recovered and purified by the recently reported biological method using mealworms. The weight average molecular weight (M ) and number average molecular weight (M ) of the biologically extracted PHA were higher than that from the chloroform extraction with comparable melting temperature (T ) and high purity. This study has successfully established a low-cost process to synthesize PHAs from BLW and subsequently confirmed the ability of mealworms to extract PHAs from various kinds of bacterial cells.

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Biological recovery and properties of poly(3-hydroxybutyrate) from Cupriavidus necator H16

Cited by 38 publications

References 22 publications

Recent Advances and Challenges towards Sustainable Polyhydroxyalkanoate (PHA) Production

Recent Advances and Challenges towards Sustainable Polyhydroxyalkanoate (PHA) Production

Simultaneous methane abatement and PHB production by Methylocystis hirsuta in a novel gas-recycling bubble column bioreactor

Production and recovery of poly(3‐hydroxybutyrate‐co‐3‐hydroxyvalerate) from biodiesel liquid waste (BLW)

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