Biological Reactive Intermediates of Mycotoxins

Hsieh, Dennis P. H.

doi:10.1007/978-1-4684-5134-4_56

Cited by 7 publications

(3 citation statements)

References 40 publications

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“…AFB 1 is a potent mutagenic and carcinogenic mycotoxin produced by Aspergillus flavus and Aspergillus parasiticus that is frequently found in foodstuffs such as corn, peanuts, and cottonseeds (Hsieh 1986;Carrillo and others 1990;Liu and Massey 1992). AFB 1 is an extensively studied model carcinogen that is highly mutagenic to Salmonella typhimurium strains TA98 and TA100 and requires metabolic activation by specific cytochrome P-450 associated enzymes to produce the highly reactive AFB 1 -8, 9-epoxide, the ultimate carcinogenic compound (Groopman and others 1992; Metha and others 1993).…”

Section: Introductionmentioning

confidence: 99%

Comparison of Antimutagenic Activity of Phenolic Compounds in Newly Harvested and Stored Common Beans Phaseolus vulgaris against Aflatoxin B1

Aparicio‐Fernández¹,

Manzo‐Bonilla²,

Loarca‐Piña³

2005

Journal of Food Science

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: Phenolic compounds were extracted from recently harvested or stored black Jamapa beans (Phaseolus vulgaris) that were subjected or not to thermal treatment. The beans studied were cropped in the same area and were from the same lot. The highest amount of condensed tannins (CT) was found in the seed coat of recently harvested beans [222.41 ± 16mg of (+)‐catechin equivalents per gram of seed coat]. After 2 yof storage, the amount of CT dropped significantly [35.8 ± 3.4 mg of (+) ‐catechin equivalents per gram of seed coat]. Thermal treatment significantly reduced the amount of CT in whole beans by approximately 70%. The raw seeds contained 13.76 ±1.2 mg of (+)‐cat‐echin equivalents per gram of seeds and a portion of CT appeared in the broth [9.4 ± 0.1 mg of (+) ‐catechin equivalents per gram of lyophilized broth]. The antimutagenic activity of these extracted phenolic compounds was tested against aflatoxin B1 (AFB1) in the Kado microsuspension assay. Newly harvested beans showed higher antimutagenic activity against AFB1 mutagenicity than stored beans. The results suggest that to take the maximum advantage of components with biological activity present in beans, they must be used fresh.

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Section: Introductionmentioning

confidence: 99%

Comparison of Antimutagenic Activity of Phenolic Compounds in Newly Harvested and Stored Common Beans Phaseolus vulgaris against Aflatoxin B1

Aparicio‐Fernández¹,

Manzo‐Bonilla²,

Loarca‐Piña³

2005

Journal of Food Science

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“…Aflatoxin B 1 (AFB 1 ), a fungal toxin that is considered to be a potent carcinogen, is produced by Aspergillus flavus and A. parasiticus and is a frequent contaminant in food stuffs such as corn, peanuts and cottonseed [1][2][3]. AFB 1 is metabolised to the 8,9-epoxide by microsomal cytochrome P450 (P450).…”

Section: Introductionmentioning

confidence: 99%

Relationship Among Antimutagenic, Antioxidant and Enzymatic Activities of Methanolic Extract from Common Beans (Phaseolus vulgaris L)

Cardador‐Martínez

Albores²,

Bah

et al. 2006

Plant Foods Hum Nutr

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Common beans are rich in phenolic compounds, which can provide health benefits to the consumer. The objective of this work was to study the relationship among antimutagenicity, antioxidant and enzymatic activities of methanolic extract and trolox by principal components multivariate analysis. Antimutagenicity of phenolic compounds present in methanolic extract from the seed coat of common beans (P. vulgaris, Flor de Mayo Bajío cultivar) and trolox against AFB1 mutagenicity were evaluated in the Salmonella typhimurium microsuspension assay. Antioxidant capacity of methanolic extract and trolox were evaluated using beta-carotene and 1,1-diphenyl-2-picryhydrazyl (DPPH) in vitro model assays. Cythrome P450 activity was measured by fluorometric assay. For phenolic extract, trolox and phenolic extract+trolox, the inhibition on AFB1 mutagenicity in tester strain TA100 was 47, 59 and 69%, respectively. While in TA98 was 39, 48 and 68%. The inhibition of phenolic compounds, trolox and phenolic compounds+trolox on cytochrome P450 (CYP450) activity was 48, 59 and 88%, respectively. Correlation analysis showed that phenolic extract and trolox have high antimutagenic and antioxidant activity and also inhibited enzymatic activity. The results suggest that the primary mechanism of action of phenolic compounds in beans against AFB1 mutagenicity may be extra-cellular in the microsuspension assay.

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“…Among them, aflatoxin B 1 (AFB 1 ) is the most toxic mycotoxin to which humans are exposed 4 5 and the most potent naturally occurring chemical liver carcinogen known 6 . Previous studies have shown that AFB 1 is a potent mutagen and hepatocarinogen 7 8 . AFB 1 is metabolized into the reactive oxygen species (ROS): aflatoxin-8,9-expoxide 9 .…”

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confidence: 97%

Distinct response of the hepatic transcriptome to Aflatoxin B1 induced hepatocellular carcinogenesis and resistance in rats

Shi

Lin

et al. 2016

Sci Rep

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Aflatoxin is a natural potent carcinogen and a major cause of liver cancer. However, the molecular mechanisms of hepatocellular carcinogenesis remain largely unexplored. In this study, we profiled global gene expression in liver tissues of rats that developed hepatocellular carcinoma (HCC) from aflatoxin B1 (AFB1) administration and those that were AFB1-resistant, as well as rats without AFB1 exposure as a control. AFB1 exposure resulted in extensive perturbation in gene expression with different functions in HCC and AFB1 resistance (AR) samples. The differentially expressed genes (DEGs) in HCC sample were enriched for cell proliferation, cell adhesion and vasculature development that largely contribute to carcinogenesis. Anti-apoptosis genes were up-regulated in HCC sample whereas apoptosis-induction genes were up-regulated in AR sample. AFB1 exposure also caused extensive alteration in expression level of lncRNAs. Among all the 4511 annotated lncRNAs, half of them were highly expressed only in HCC sample and up-regulated a group of protein-coding genes with cancer-related functions: apoptosis regulation, DNA repair, and cell cycle. Intriguingly, these genes were down-regulated by lncRNAs highly expressed in AR sample. Collectively, apoptosis is the critical biological process for carcinogenesis in response to AFB1 exposure through changes in expression level of both protein-coding and lncRNA genes.

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Biological Reactive Intermediates of Mycotoxins

Cited by 7 publications

References 40 publications

Comparison of Antimutagenic Activity of Phenolic Compounds in Newly Harvested and Stored Common Beans Phaseolus vulgaris against Aflatoxin B1

Comparison of Antimutagenic Activity of Phenolic Compounds in Newly Harvested and Stored Common Beans Phaseolus vulgaris against Aflatoxin B1

Relationship Among Antimutagenic, Antioxidant and Enzymatic Activities of Methanolic Extract from Common Beans (Phaseolus vulgaris L)

Distinct response of the hepatic transcriptome to Aflatoxin B1 induced hepatocellular carcinogenesis and resistance in rats

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