Biological Properties of Ti-Si-C-O-N Thin Films

Abstract

The main aim of this work was to study the feasibility of new coatings for medical devices. Ti-Si-C-O-N films were deposited by DC unbalanced reactive magnetron sputtering, using different oxygen and nitrogen partial pressure ratios (pO2/pN2). Surface properties were also analysed. Staphylococcus epidermidis was used to study biofilm formation and cytotoxicity was determined using fibroblasts. Surface morphology changed with the increase of pO2/pN2. Samples of high hydrophobicity displayed opposite behaviour in terms of biofilm formation, presenting the highest and lowest biomass. Moreover, the sample with the highest Ti content was the one, with the lowest biofilm amount, raising the possibility of a correlation between Ti and biofilm formation capability. In fact, this sample also displayed the highest degree of cytotoxicity (near 35%). This work shows the feasibility of the proposed coatings and highlights the importance of joining together both biological properties (biofilm formation and cytotoxicity) with the surface characterization.

Hip prosthesis biomaterials: a challenge in prevention of biofilm formation

“…Cross-linked polyethylene combined with vitamin E-blended and a poly(2methacryloyloxyethyl phosphorylcholine) layer coatings, silver-zirconium carbonitride (Ag-ZrCN) films, acylase-containing polyurethane coatings and titanium-silicon-carbon-oxygen-nitrogen (Ti-Si-C-O-N) films had antibacterial activity. However, nanocrystalline diamond coatings did not presented the same effect (HENRIQUES et al, 2009;LEWIS et al, 2010;FERRERI et al, 2015;KYOMOTO et al, 2015;GROVER et al, 2016).…”

“…Biomaterials used on hip arthroplasties were researched to identify their possible influence on biofilm formation prevention on hip prosthesis. The presence of factors that interfere on biofilm formation was evidenced: metal roughness, antibiotic coatings action, difference in biofilm adhesion on distinct biomaterials, ions action, different concentrations of titanium and silver, coating activity of HD-CLPE(VE) with PMPC and acylase activity in disrupting quorum-sensing signals (RAMAGE et al, 2003;PARVIZI et al, 2004;TOMÁŠ;NACHTNEBL;OTIEPKA, 2007;HENRIQUES et al, 2009;HOSMAN et al, 2009HOSMAN et al, , 2011NURYASTUTI et al, 2011;GÓMEZ-BARRENA et al, 2012;NEUT et al, 2012NEUT et al, , 2015LASS et al, 2014;FERRERI et al, 2015;KYOMOTO et al, 2015;GROVER et al, 2016).…”

“…Various studies were performed to develop and evaluate new coatings with the ability to minimize microbial colonization (PARVIZI et al, 2004;HENRIQUES et al, 2009;LEWIS et al, 2010;NEUT et al, 2012NEUT et al, , 2015FERRERI et al, 2015;KYOMOTO et al, 2015;GROVER et al, 2016). Silver-zirconium carbonitride (Ag-ZrCN) thin films and cross-linked polyethylene combined with vitamin E-blended and a poly(2methacryloyloxyethyl phosphorylcholine) layer -(HD-CLPE(VE) with PMPC) coatings presented antimicrobial activity (FERRERI et al, 2015;KYOMOTO et al, 2015).…”

“…Moreover, the biomaterial developed with an acylase-containing polyurethane coating disrupted quorum-sensing signals 1397 Hip prosthesis biomaterials… MARQUES, D. M. et al responsible by communication and progression of biofilm formation (GROVER et al, 2016). On the other hand, nanocrystalline diamond coatings did not avoid biofilm development, and titaniumsilicon-carbon-oxygen-nitrogen (Ti-Si-C-O-N) coatings presented lower amount of biofilm (biomass), yet high cytotoxicity (HENRIQUES et al, 2009;LEWIS et al, 2010).…”