Biological properties of the venom from the scorpionfish (Scorpaena plumieri) and purification of a gelatinolytic protease

Carrijo, Linda Christian; Andrich, Filipe; Lima, Maria Elena de; Cordeiro, Marta N.; Richardson, Michael; Figueiredo, Suely G.

doi:10.1016/j.toxicon.2005.01.021

Cited by 53 publications

(42 citation statements)

References 31 publications

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“…Due to their role in apoptosis and energy metabolism, recently there is growing interest to explore the potential of the mitochondria as a tool in cancer treatment. Some structural and functional alterations to the mitochondria in cancer cells render it possible to develop approaches to selectively affect cancer cells 6 Hepat Mon. 2017; 17(11):e11842.…”

Section: Discussionmentioning

confidence: 99%

“…Less toxic species like fish represent a valuable source of phar-macological compounds that may be a potential candidate for cancer treatment. Fish venom potentially include important source of pharmacological and physiological compounds that target integral processes within the human body more than many drugs (5,6). Stonefish belongs to the genus Synanceia and resides in the shallow waters of the Pacific and Indian Oceans.…”

Section: Introductionmentioning

confidence: 99%

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Persian Gulf Stonefish (Pseudosynanceia melanostigma) Venom Fractions Selectively Induce Apoptosis on Cancerous Hepatocytes from Hepatocellular Carcinoma Through ROS-Mediated Mitochondrial Pathway

et al. 2017

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Background: Globally, hepatocellular carcinoma (HCC) is the 3rd leading reason for mortality associated with cancer and the 6th most widespread malignant tumor. Objectives: This study aims to investigate selective toxicity of venom fractions of Pseudosynanceia melanostigma, commonly known as stonefish, on hepatocytes and mitochondria obtained from diethylnitrosamine (DEN) induced hepatocellular carcinoma (HCC). Methods: Different dilutions of extracted fractions from crude stonefish venom were treated on hepatocytes and mitochondria isolated from a rat model of hepatocellular carcinoma induced by diethylnitrosamine (DEN). In response to stonefish venom fractions, mitochondrial related parameters including generation of reactive oxygen species (ROS), mitochondrial membrane potential (MMP) collapse, mitochondrial swelling, cytochrome c release, activation of caspase 3, and induction of apoptosis were investigated. Results: Our results demonstrate that for the first time, fraction 3 of Pseudosynanceia melanostigma treatment on cancerous mitochondria had a significant accumulation of reactive oxygen species (ROS). In addition, mitochondrial membrane potential (MMP) disruption, mitochondrial swelling, and cytochrome c release increased. Moreover, fraction 3 induced selective toxicity only in cancerous hepatocytes from the HCC but not those from healthy cells. Additional research also determined a significant increase in activation of caspase 3 and induction of apoptosis. Conclusions: In conclusion, this study provides evidence that fraction 3 of Pseudosynanceia melanostigma venom selectively induces apoptosis in cancerous hepatocytes from HCC through a ROS-mediated mitochondrial pathway.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Persian Gulf Stonefish (Pseudosynanceia melanostigma) Venom Fractions Selectively Induce Apoptosis on Cancerous Hepatocytes from Hepatocellular Carcinoma Through ROS-Mediated Mitochondrial Pathway

et al. 2017

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“…Additionally, local edema and erythema may arise as consequence of envenoming. The systemic symptoms of cardiotoxic and vasorelaxant effects are more severe, resulting in a drastic drop of blood pressure (22)(23)(24)(25). Moreover, the observed increase in the epithelial permeability of the bronchi and alveoli, similar to the observed erythema and hemorrhages, indicate a loss of cell-matrix interactions (22,23).…”

Section: Discussionmentioning

confidence: 99%

“…These symptoms indicate the presence of toxins interfering with integrin-mediated cell-matrix interactions. Some of these symptoms have been observed after envenomation by the scorpionfish (Scorpaena plumieri) (22)(23)(24)(25) and have encouraged us to analyze this fish venom for potential integrin inhibitors.…”

mentioning

confidence: 99%

Plumieribetin, a Fish Lectin Homologous to Mannose-binding B-type Lectins, Inhibits the Collagen-binding α1β1 Integrin

Evangelista

Andrich

Rezende

et al. 2009

Journal of Biological Chemistry

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Recently, a few fish proteins have been described with a high homology to B-type lectins of monocotyledonous plants. Because of their mannose binding activity, they have been ascribed a role in innate immunity. By screening various fish venoms for their integrin inhibitory activity, we isolated a homologous protein from the fin stings and skin mucus of the scorpionfish (Scorpaena plumieri). This protein inhibits ␣1␤1 integrin binding to basement membrane collagen IV. By protein chemical and spectroscopic means, we demonstrated that this fish protein, called plumieribetin, is a homotetramer and contains a high content of anti-parallel ␤ strands, similar to the mannose-binding monocot B-lectins. It lacks both N-linked glycoconjugates and common O-glycan motifs. Despite its B-lectin-like structure, plumieribetin binds to ␣1␤1 integrin irrespective of N-glycosylation, suggesting a direct protein-protein interaction. This interaction is independent of divalent cations. On the cellular level, plumieribetin failed to completely detach hepatocarcinoma HepG2 cells and primary arterial smooth muscle cells from the collagen IV fragment CB3. However, plumieribetin weakened the cell-collagen contacts, reduced cell spreading, and altered the actin cytoskeleton, after the compensating ␣2␤1 integrin was blocked. The integrin inhibiting effect of plumieribetin adds a new function to the B-lectin family, which is known for pathogen defense.

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“…Consequently, accidents involving humans could represent significant economic and health problems. Therefore, it is important to perform further studies on this subject to improve the knowledge on bioactive toxins of these venoms, which may advance therapeutic techniques (2). The present text also represents the first statement on the venomous glandular tissue of a Scorpaenidae species from the northeastern Atlantic.…”

Section: Dear Sirmentioning

confidence: 93%

Are weight, length and amount of venom related in scorpionfish?

Vieira

Barreiros

2010

J. Venom. Anim. Toxins incl. Trop. Dis

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Biological properties of the venom from the scorpionfish (Scorpaena plumieri) and purification of a gelatinolytic protease

Cited by 53 publications

References 31 publications

Persian Gulf Stonefish (Pseudosynanceia melanostigma) Venom Fractions Selectively Induce Apoptosis on Cancerous Hepatocytes from Hepatocellular Carcinoma Through ROS-Mediated Mitochondrial Pathway

Persian Gulf Stonefish (Pseudosynanceia melanostigma) Venom Fractions Selectively Induce Apoptosis on Cancerous Hepatocytes from Hepatocellular Carcinoma Through ROS-Mediated Mitochondrial Pathway

Plumieribetin, a Fish Lectin Homologous to Mannose-binding B-type Lectins, Inhibits the Collagen-binding α1β1 Integrin

Are weight, length and amount of venom related in scorpionfish?

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