Biological pretreatment of corn stover for enhancing enzymatic hydrolysis using Bacillus sp. P3

Abstract

The biological pretreatment for the enzymatic hydrolysis of lignocellulosic biomasses depends exclusively on the effective pretreatment process. Herein, we report a significant enhancement of enzymatic saccharification obtained with corn stover using a bacterial strain Bacillus sp. P3. The hemicellulose removal from corn stover by the strain Bacillus sp. P3 was evaluated for enhancing subsequent enzymatic hydrolysis. Therefore, our study revealed that an alkaline-resistant xylanase as well as other enzymes produced by Bacillus sp. P3 in fermentation broth led to a substantially enhanced hemicellulose removal rate from corn stover within pH 9.36–9.68. However, after a 20-day pretreatment of corn stover by the strain P3, the glucan content was increased by 51% and the xylan content was decreased by 35%. After 72 h of saccharification using 20 U/g of commercial cellulase, the yield of reducing sugar released from 20-day pretreated corn stover was increased by 56% in comparison to the untreated corn stover. Therefore, the use of the strain P3 could be a promising approach to pretreat corn stover for enhancing the enzymatic hydrolysis process of industrial bioenergy productions.

Unleashing the Ligno-Hemicellulolytic Enzyme Potential of Pyrenophora phaeocomes S-1: A Solid State Fermentation Approach for Efficient Delignification of Corn Stover

“…At different time points (10,20,30,40, and 60 days) of incubation, the flasks were withdrawn in duplicate, and the contents were mixed with distilled water. The crude enzyme extract (CEE) was then extracted from the mixture as described in section 2.3.…”

“…Some research groups have explored co-culturing techniques using commercially known laccase (C. comatus) and xylanase (T. reesei) producers, achieving peak productivities of 2180 U/ml and 160 U/ml, respectively, after 5 days of incubation [29]. The early appearance of high levels of laccases and hemicellulases during fermentation facilitates the delignification process and reduces the duration of biological treatment [30]. The availability of high titers of lingo-hemicellulases at the early stages of fermentation appears to be advantageous for the biological pretreatment of corn stover.…”

“…Microbial treatment offers targeted action on the lignin-hemicellulose complex and provides the advantage of treated biomass with improved hydrolysis activities and a variety of ligno, hemicellulo, or cellulolytic enzyme systems. The productivity profiles of ligno-hemicellulolytic enzymes are critical in determining the efficiency of the biological pretreatment reaction [10,28,30].…”

Enhancement of saccharification of corn stover by cellulolytic enzyme produced from biomass-degrading bacteria

“…The biomass composition analysis was performed as mentioned in Wu et al (2021), in which cellulose and hemicellulose were analyzed by measuring the glucan and xylan contents. The Klason lignin analysis was performed by anthrone-sulfuric acid and orcinolhydrochloric acid methods.…”

“…Moreover, the study revealed Bacillus sp. produced maximum CMCase (17.8 U g -1 ) and xylanase (195.8 U g -1 ) from corn stover (0.5% w/v) among the various biomasses and they used corn stover for further study (Wu et al 2021).…”