Biological preparation of highly effective immunomagnetic beads for the separation, concentration, and detection of pathogenic bacteria in milk

Lim, Min-Cheol; Lee, Gwan-Hyung; Huynh, Duyen Thi Ngoc; Hong, Chae-Eun; Park, Soo Yeon; Jung, Jong-Yun; Park, Cheon-Seok; Ko, Seong Young; Kim, Young-Rok

doi:10.1016/j.colsurfb.2016.05.077

Cited by 41 publications

(27 citation statements)

References 51 publications

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“…Immunomagnetic separation (IMS) is effective for identifying bacteria from foods due to selective separating target molecules from a heterogeneous matrix [12][13][14][15]. A target-specific antibody on the surface of a magnetic bead is a crucial factor for increasing the yield of selective separation and the concentration of the antigenic target [16][17][18]. Especially, the magnetic beads could be successfully separated and concentrated from the large volume of the sample solution by applying the external magnetic field.…”

Section: Introductionmentioning

confidence: 99%

Detection of Escherichia coli O157:H7 Using Automated Immunomagnetic Separation and Enzyme-Based Colorimetric Assay

Park

et al. 2020

Sensors

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The food industry requires rapid and simple detection methods for preventing harm from pathogenic bacteria. Until now, various technologies used to detect foodborne bacteria were time-consuming and laborious. Therefore, we have developed an automated immunomagnetic separation combined with a colorimetric assay for the rapid detection of E. coli O157:H7 in food samples. The colorimetric detection method using enzymatic reaction is fascinating because of its simplicity and rapidity and does not need sophisticated devices. Moreover, the proposed procedures for the detection of bacteria in food take less than 3 h including pre-enrichment, separation and detection steps. First, target-specific immunomagnetic beads were introduced to contaminated milk in a pre-enrichment step. Second, the pre-enriched sample solution containing target bacteria bound on immunomagnetic beads was injected into an automated pretreatment system. Subsequently, the immunomagnetic beads along with target bacteria were separated and concentrated into a recovery tube. Finally, released β-galactosidase from E. coli O157:H7 after lysis was reacted with chlorophenol red β-galactopyranoside (CPRG) used as a substrate and the colorimetric change of CPRG was determined by absorbance measuring or the naked eye. By the proposed approach in this study, we could detect 3 × 102 CFU/mL of E. coli O157:H7 from a milk sample within 3 h.

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Section: Introductionmentioning

confidence: 99%

Detection of Escherichia coli O157:H7 Using Automated Immunomagnetic Separation and Enzyme-Based Colorimetric Assay

Park

et al. 2020

Sensors

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“…In recent years, magnetic nanoparticles based technology with multidisciplinary and remarkable characteristics offers great possibilities for development for new analytical methodology [24–26]. Immunomagnetic beads (IMBs) due to the distinct physical and chemical properties, such as uniform diameter particles, large surface area, simplicity, superior compatibility, good sensitivity, and easy operation that can be homogenously distributed in colloidal suspension, have been widely applied in various fields, including nucleic acid extraction, peptide and protein analysis, and antibody purification [27–31]. In addition, IMBs have good biocompatibility for coupling with antibodies or small molecules and larger surface area than the flat base of a microtiter plate, which immobilizes more numbers of “active molecules” and enhances the sensitivity of the immunoassay.…”

Section: Introductionmentioning

confidence: 99%

Rapid determination of aflatoxin B1 by an automated immunomagnetic bead purification sample pretreatment method combined with high‐performance liquid chromatography

Zhang

Liu

et al. 2020

J of Separation Science

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We aimed to establish an automated versatile sample preconcentration method based on the modified immunomagnetic beads, which was utilized to enrich for aflatoxin B1 from the matrices. The critical main parameters affecting the extraction efficiency, such as usage amount of immunomagnetic beads, reaction time, elution time, and blending way were investigated. Under the optimized conditions, the content of aflatoxin B1 was analyzed by high‐performance liquid chromatography, the mobile phase consists of water–acetonitrile–methanol (42:18:10, v/v/v), and fluorescence detection was performed with excitation and emission wavelengths at 360 and 440 nm, respectively. Moreover, the performance of preconcentration method was compared with the conventional method based on the immunoaffinity column. The accuracy of two clean‐up methods was within the error range. In addition, the stability and recyclability of the immunomagnetic beads was studied by recycling them five times. The results for the respective analysis in various samples demonstrated that the developed extraction platform provides a promising approach that is simple, rapid, sensitive, and easy to use.

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“…In the literature, magnetic bead separation processes have been studied for soil clean up (Funada, 2018), wastewater treatment (Lee et al, 2015;Lei et al, 2016;Fuks et al, 2018;Pal et al,. 2018), concentrating pathogenic bacteria (Almand et al, 2016;Lim et al, 2016), viruses (Satoh et al, 2003Almand et al, 2016;Sakudo et al, 2016), and DNA (Satoh et al, 2008;Bordelon et al, 2013). DNA adsorption to the surface of magnetic beads has been mainly used to extract DNA from the sample matrix with higher concentration and lower impurities (Berensmeier, 2006).…”

Section: Introductionmentioning

confidence: 99%

Kaplanmış Manyetik Kürelere DNA Adsorpsiyonu: Fiziksel ve Kimyasal Adsorpsiyonun Karşılaştırılması

Bayraç

Sarikaya³

et al. 2019

Erzincan Üniversitesi Fen Bilimleri Enstitüsü Dergisi

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Based on their unique properties various iron magnetic nanoparticles have proved to be excellent nanomaterials for applications in separation and concentration process. Immobilization of deoxyribonucleic acid (DNA) molecules on the magnetic beads are acutely important and have potential uses in many techniques such as DNA extraction, concentration, biosensors, microarrays and next generation sequencing. In this study the adsorption of single stranded DNA (ssDNA) via poly-l-lysine coated iron oxide magnetic beads was performed under varying conditions of poly-l-lysine amount, initial DNA concentration, ionic strength, bonding type and length of DNA. The adsorption process was examined via Langmuir and Freundlich isotherm models. The ionic interaction between negatively charged DNA and positively charged surface of magnetic beads showed multilayer adsorption with Freundlich adsorption isotherm, covalent bonding between modified DNA and surface by crosslinking provided higher adsorption efficiency with Langmuir adsorption isotherm. Both adsorption methods provided magnetic beads with favorable adsorption of ssDNA.

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Biological preparation of highly effective immunomagnetic beads for the separation, concentration, and detection of pathogenic bacteria in milk

Cited by 41 publications

References 51 publications

Detection of Escherichia coli O157:H7 Using Automated Immunomagnetic Separation and Enzyme-Based Colorimetric Assay

Detection of Escherichia coli O157:H7 Using Automated Immunomagnetic Separation and Enzyme-Based Colorimetric Assay

Rapid determination of aflatoxin B1 by an automated immunomagnetic bead purification sample pretreatment method combined with high‐performance liquid chromatography

Kaplanmış Manyetik Kürelere DNA Adsorpsiyonu: Fiziksel ve Kimyasal Adsorpsiyonun Karşılaştırılması

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