Biological phosphorus uptake under anoxic and aerobic conditions

Kerrn-Jespersen, Jens Peter; Henze, Mogens

doi:10.1016/0043-1354(93)90171-d

Cited by 284 publications

(96 citation statements)

References 3 publications

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“…This observation was slightly different from results reported in previous studies suggesting the presence of two PAO types, denitrifying PAOs and PAOs. These studies showed that denitrifying P uptake ceased, despite the presence of available nitrate due to internal PHA depletion of denitrifying PAOs, and P uptake by other PAOs continued again when oxygen was supplied (12,23,51,52). In the present study, our SBR operation showed that nitrite concentrations in the bulk solution increased concurrently with the decrease (Fig.…”

Section: Discussionsupporting

confidence: 54%

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Characterization of the Denitrification-Associated Phosphorus Uptake Properties of “Candidatus Accumulibacter phosphatis” Clades in Sludge Subjected to Enhanced Biological Phosphorus Removal

Kim

Lee

et al. 2013

Appl Environ Microbiol

124

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bTo characterize the denitrifying phosphorus (P) uptake properties of "Candidatus Accumulibacter phosphatis," a sequencing batch reactor (SBR) was operated with acetate. The SBR operation was gradually acclimated from anaerobic-oxic (AO) to anaerobic-anoxic-oxic (A2O) conditions by stepwise increases of nitrate concentration and the anoxic time. The communities of "Ca. Accumulibacter" and associated bacteria at the initial (AO) and final (A2O) stages were compared using 16S rRNA and polyphosphate kinase genes and using fluorescence in situ hybridization (FISH). The acclimation process led to a clear shift in the relative abundances of recognized "Ca. Accumulibacter" subpopulations from clades IIA > IA > IIF to clades IIC > IA > IIF, as well as to increases in the abundance of other associated bacteria (Dechloromonas [from 1.2% to 19.2%] and "Candidatus Competibacter phosphatis" [from 16.4% to 20.0%]), while the overall "Ca. Accumulibacter" abundance decreased (from 55.1% to 29.2%). A series of batch experiments combined with FISH/microautoradiography (MAR) analyses was performed to characterize the denitrifying P uptake properties of the "Ca. Accumulibacter" clades. In FISH/MAR experiments using slightly diluted sludge (ϳ0.5 g/liter), all "Ca. Accumulibacter" clades successfully took up phosphorus in the presence of nitrate. However, the "Ca. Accumulibacter" clades showed no P uptake in the presence of nitrate when the sludge was highly diluted (ϳ0.005 g/liter); under these conditions, reduction of nitrate to nitrite did not occur, whereas P uptake by "Ca. Accumulibacter" clades occurred when nitrite was added. These results suggest that the "Ca. Accumulibacter" cells lack nitrate reduction capabilities and that P uptake by "Ca. Accumulibacter" is dependent upon nitrite generated by associated nitrate-reducing bacteria such as Dechloromonas and "Ca. Competibacter."

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Section: Discussionsupporting

confidence: 54%

“…Many previous investigations have demonstrated that successful EBPR has been achieved using nitrate as a final electron acceptor (21)(22)(23)(24)(25), which suggests that "Ca. Accumulibacter" clades may have the ability to take up P using nitrate (8).…”

mentioning

confidence: 99%

Characterization of the Denitrification-Associated Phosphorus Uptake Properties of “Candidatus Accumulibacter phosphatis” Clades in Sludge Subjected to Enhanced Biological Phosphorus Removal

Kim

Lee

et al. 2013

Appl Environ Microbiol

124

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“…However, EBPR processes combined with biological nitrogen removal processes have a drawback in that the organic substrate used is a limiting factor for the activities of both PAOs and denitrifying bacteria. Recently, the occurrence of DNPAOs capable of utilizing nitrite or nitrate instead of oxygen as an electron acceptor for phosphorus uptake has been reported (2,10,13). The use of DNPAOs in biological nutrient removal processes is advantageous because identical organic substrates such as acetate can be efficiently used as the energy source for both nitrogen and phosphorus removals.…”

mentioning

confidence: 99%

Diversity of Nitrite Reductase Genes in “ Candidatus Accumulibacter phosphatis”-Dominated Cultures Enriched by Flow-Cytometric Sorting

Miyauchi

Oki

Aoi

et al. 2007

Appl Environ Microbiol

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"Candidatus Accumulibacter phosphatis" is considered a polyphosphate-accumulating organism (PAO) though it has not been isolated yet. To reveal the denitrification ability of this organism, we first concentrated this organism by flow cytometric sorting following fluorescence in situ hybridization (FISH) using specific probes for this organism. The purity of the target cells was about 97% of total cell count in the sorted sample. The PCR amplification of the nitrite reductase genes (nirK and nirS) from unsorted and sorted cells was performed. Although nirK and nirS were amplified from unsorted cells, only nirS was detected from sorted cells, indicating that "Ca. Accumulibacter phosphatis" has nirS. Furthermore, nirS fragments were cloned from unsorted (Ba clone library) and sorted (Bd clone library) cells and classified by restriction fragment length polymorphism analysis. The most dominant clone in clone library Ba, which represented 62% of the total number of clones, was not found in clone library Bd. In contrast, the most dominant clone in clone library Bd, which represented 59% of the total number of clones, represented only 2% of the total number of clones in clone library Ba, indicating that this clone could be that of "Ca. Accumulibacter phosphatis." The sequence of this nirS clone exhibited less than 90% similarity to the sequences of known denitrifying bacteria in the database. The recovery of the nirS genes makes it likely that "Ca. Accumulibacter phosphatis" behaves as a denitrifying PAO capable of utilizing nitrite instead of oxygen as an electron acceptor for phosphorus uptake.

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“…Entonces, el fenómeno de liberación de fósforo se detendrá, y será sustituido por el de captación. Varios investigadores han descrito la captación de fósforo en condiciones anóxicas empleando nitrato como donador de electrones (Wentzel et al, 1985;Comeau et al, 1987;Kerrn-Jespersen y Henze, 1993;Kerrn-Jespersen et al, 1994), explicando que cuando se introducen nitratos a la fase anaerobia, la liberación de P se ve inhibida ya que se produce una respiración anóxica a base de nitratos, y el fenómeno que entonces predominará será el de bioacumulación de fósforo. De esta forma, es necesario conseguir un ambiente anaerobio estricto para que se produzca la liberación de fósforo y captación de materia orgánica, ya que la presencia de óxidos de nitrógeno aumentan el potencial redox de la fase anaerobia, disminuyendo de esta forma la cinética de liberación de fósforo.…”

Section: Nitratos Y Nitritosunclassified

Consideraciones de diseño para la eliminación biológica de fósforo empleando procesos biopelícula

Castro

Monzón

1999

ing.agua

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RESUMEN:El siguiente artículo es un resumen del estado del arte de la Eliminación Biológica de Fósforo (EBF) empleando procesos de biopelícula. En él se describen minuciosamente los mecanismos que intervienen en la eliminación biológica de este elemento, así como las teorías actualmente aceptadas. Se presentan los procesos biopelícula actualmente desarrollados, así como los consideraciones de diseño para reactores biopelícula, y la forma de trabajo necesaria para conseguir la EBF. INTRODUCCIÓNTradicionalmente, el sistema empleado para eliminar fósforo de las aguas residuales ha sido la precipitación química, práctica que está siendo sustituida por los sistemas biológicos, ya que a través de ellos es posible obtener bajos niveles de fósforo efluente sin la neceéis-dad de incurrir en altos gastos de operación y mantenimiento. Los procesos de cultivo en suspensión de eliminación biológica de fósforo han sido los primeros en desarrollarse y explotarse a gran escala, llegándose a implantar sistemas mixtos que combinan la eliminación biológica de fósforo con la precipitación química. Hasta hace poco tiempo se consideraba sumamente difícil, sino imposible, eliminar fósforo de las aguas residuales empleando procesos de biopelícula (Iwai y Kitao, 1994). Hoy día, la eliminación biológica de fós-foro empleando estos procesos, es posible gracias a la aplicación del concepto de operación secuencial (Sequencing Batch Reactor SBR). Las recientes publicaciones sobre eliminación biológica de fósforo empleando procesos biopelícula, demuestran el interés existente en la comunidad científica internacional por aprovechar las ventajas que brindan estos procesos, frente al sistema convencional de cultivo en suspensión, aunque de momento, la eliminación biológica de fósforo empleando procesos biopelícula se encuentra en fase experimental, y aún no ha sido establecida una aproximación de diseño específica. De esta forma, el diseño de procesos biopelícula de eliminación biológica de fósforo se lleva a cabo sobre la base de resultados experimentales de plantas pilotos, así como de una serie de factores, muchos de ellos comunes al diseño de sistemas de fangos activos. LA ELIMINACIÓN BIOLÓGICA DE FÓSFORO (EBF)Para lograr bajas concentraciones de fósforo (P) en un efluente por vía biológica, es necesario conseguir que los microorganismos acumulen este elemento más allá de sus necesidades metabólicas de crecimiento. Así, la EBF se basa en exponer la biomasa a ciclos anaerobio-aerobio. En el tiempo de contacto anaerobio, las bacterias que intervienen en la eliminación biológica del fósforo, usan sus reservas intracelulares de polifosfatos como fuente de energía, y almacenan sustratos orgánicos simples, como los ácidos grasos volátiles (AGV), lo que favorece la liberación de fósforo en la fase anaerobia. Durante la fase aerobia, las bacterias usan sus reservas de carbono como fuente de energía y acumulan más fósforo que el liberado en la fase previa, almacenando este elemento muy por encima de sus necesidades estequiométricas. Al finalizar esta...

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Biological phosphorus uptake under anoxic and aerobic conditions

Cited by 284 publications

References 3 publications

Characterization of the Denitrification-Associated Phosphorus Uptake Properties of “Candidatus Accumulibacter phosphatis” Clades in Sludge Subjected to Enhanced Biological Phosphorus Removal

Characterization of the Denitrification-Associated Phosphorus Uptake Properties of “Candidatus Accumulibacter phosphatis” Clades in Sludge Subjected to Enhanced Biological Phosphorus Removal

Diversity of Nitrite Reductase Genes in “ Candidatus Accumulibacter phosphatis”-Dominated Cultures Enriched by Flow-Cytometric Sorting

Consideraciones de diseño para la eliminación biológica de fósforo empleando procesos biopelícula

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