Biological phosphorus removal by pure culture of Lampropedia spp.

Stante, Loredana; Cellamare, C. M.; Malaspina, F.; Bortone, G.; Tilche, A.

doi:10.1016/s0043-1354(96)00351-x

Cited by 80 publications

(57 citation statements)

References 25 publications

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“…(Suresh et al, 1985), Arthrobacter globiformis (Shoda et al, 1980) and Lampropedia spp. (Stante et al, 1997). Nevertheless, these isolates could not store substrate (i.e.…”

Section: Introductionmentioning

confidence: 95%

Micropruina glycogenica gen. nov., sp. nov., a new Gram-positive glycogen-accumulating bacterium isolated from activated sludge.

Shintani¹,

Liu²,

Hanada³

et al. 2000

International Journal of Systematic and Evolutionary Microbiology

114

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“…(Suresh et al, 1985), Arthrobacter globiformis (Shoda et al, 1980) and Lampropedia spp. (Stante et al, 1997). Nevertheless, these isolates could not store substrate (i.e.…”

Section: Introductionmentioning

confidence: 95%

Micropruina glycogenica gen. nov., sp. nov., a new Gram-positive glycogen-accumulating bacterium isolated from activated sludge.

Shintani¹,

Liu²,

Hanada³

et al. 2000

International Journal of Systematic and Evolutionary Microbiology

114

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“…(31), Microlunatus phosphovorus (36), Lampropedia spp. (40), and Gram-positive Actinobacteria (24). However, those organisms do not exhibit all of the characteristics of the EBPR biochemistry model.…”

mentioning

confidence: 99%

Analysis of the Fine-Scale Population Structure of “ Candidatus Accumulibacter phosphatis” in Enhanced Biological Phosphorus Removal Sludge, Using Fluorescence In Situ Hybridization and Flow Cytometric Sorting

Kim

Lee

Kim

et al. 2010

Appl Environ Microbiol

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To investigate the fine-scale diversity of the polyphosphate-accumulating organisms (PAO) "Candidatus Accumulibacter phosphatis" (henceforth referred to as "Ca. Accumulibacter"), two laboratory-scale sequencing batch reactors (SBRs) for enhanced biological phosphorus removal (EBPR) were operated with sodium acetate as the sole carbon source. During SBR operations, activated sludge always contained morphologically different "Ca. Accumulibacter" strains showing typical EBPR performances, as confirmed by the combined technique of fluorescence in situ hybridization (FISH) and microautoradiography (MAR). Fragments of "Ca. Accumulibacter" 16S rRNA genes were retrieved from the sludge. Phylogenetic analyses together with sequences from the GenBank database showed that "Ca. Accumulibacter" 16S rRNA genes of the EBPR sludge were clearly differentiated into four "Ca. Accumulibacter" clades, Acc-SG1, Acc-SG2, Acc-SG3, and Acc-SG4. The specific FISH probes Acc444, Acc184, Acc72, and Acc119 targeting these clades and some helpers and competitors were designed by using the ARB program. Microbial characterization by FISH analysis using specific FISH probes also clearly indicated the presence of different "Ca. Accumulibacter" cell morphotypes. Especially, members of Acc-SG3, targeted by probe Acc72, were coccobacillus-shaped cells with a size of approximately 2 to 3 m, while members of Acc-SG1, Acc-SG2, and Acc-SG4, targeted by Acc444, Acc184, and Acc119, respectively, were coccus-shaped cells approximately 1 m in size. Subsequently, cells targeted by each FISH probe were sorted by use of a flow cytometer, and their polyphosphate kinase 1 (ppk1) gene homologs were amplified by using a ppk1-specific PCR primer set for "Ca. Accumulibacter." The phylogenetic tree based on sequences of the ppk1 gene homologs was basically congruent with that of the 16S rRNA genes, but members of Acc-SG3 with a distinct morphology comprised two different ppk1 genes. These results suggest that "Ca. Accumulibacter" strains may be diverse physiologically and ecologically and represent distinct populations with genetically determined adaptations in EBPR systems.

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“…However, evidence indicating that Acinetobacter may not be responsible for EBPR includes pure culture performances not correlating with biological models (7,38) and analyses of EBPR bacterial communities indicating that Acinetobacter microorganisms are not present in high enough numbers to account for EBPR (7,14,24,30,41). Investigations of other EBPR-associated microorganisms are limited, although there has been some interest in grampositive bacteria such as Microlunatus phosphovorus (33,39), the gram-negative Lampropedia (35), and the Actinobacteria and ␣ Proteobacteria (25). However, there is no general consensus that these bacteria are examples of PAOs, and indeed Mino et al (31) concluded that rather than there being a single dominant PAO, several different bacterial groups may be important.…”

mentioning

confidence: 99%

Identification of Polyphosphate-Accumulating Organisms and Design of 16S rRNA-Directed Probes for Their Detection and Quantitation

Crocetti

Hugenholtz

Bond

et al. 2000

Appl Environ Microbiol

661

530

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Laboratory-scale sequencing batch reactors (SBRs) as models for activated sludge processes were used to study enhanced biological phosphorus removal (EBPR) from wastewater. Enrichment for polyphosphateaccumulating organisms (PAOs) was achieved essentially by increasing the phosphorus concentration in the influent to the SBRs. Fluorescence in situ hybridization (FISH) using domain-, division-, and subdivision-level probes was used to assess the proportions of microorganisms in the sludges. The A sludge, a high-performance P-removing sludge containing 15.1% P in the biomass, was comprised of large clusters of polyphosphate-containing coccobacilli. By FISH, >80% of the A sludge bacteria were ␤-2 Proteobacteria arranged in clusters of coccobacilli, strongly suggesting that this group contains a PAO responsible for EBPR. The second dominant group in the A sludge was the Actinobacteria. Clone libraries of PCR-amplified bacterial 16S rRNA genes from three high-performance P-removing sludges were prepared, and clones belonging to the ␤-2 Proteobacteria were fully sequenced. A distinctive group of clones (sharing >98% sequence identity) related to Rhodocyclus spp. (94 to 97% identity) and Propionibacter pelophilus (95 to 96% identity) was identified as the most likely candidate PAOs. Three probes specific for the highly related candidate PAO group were designed from the sequence data. All three probes specifically bound to the morphologically distinctive clusters of PAOs in the A sludge, exactly coinciding with the ␤-2 Proteobacteria probe. Sequential FISH and polyphosphate staining of EBPR sludges clearly demonstrated that PAO probe-binding cells contained polyphosphate. Subsequent PAO probe analyses of a number of sludges with various P removal capacities indicated a strong positive correlation between P removal from the wastewater as determined by sludge P content and number of PAO probe-binding cells. We conclude therefore that an important group of PAOs in EBPR sludges are bacteria closely related to Rhodocyclus and Propionibacter.

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Biological phosphorus removal by pure culture of Lampropedia spp.

Cited by 80 publications

References 25 publications

Micropruina glycogenica gen. nov., sp. nov., a new Gram-positive glycogen-accumulating bacterium isolated from activated sludge.

Micropruina glycogenica gen. nov., sp. nov., a new Gram-positive glycogen-accumulating bacterium isolated from activated sludge.

Analysis of the Fine-Scale Population Structure of “ Candidatus Accumulibacter phosphatis” in Enhanced Biological Phosphorus Removal Sludge, Using Fluorescence In Situ Hybridization and Flow Cytometric Sorting

Identification of Polyphosphate-Accumulating Organisms and Design of 16S rRNA-Directed Probes for Their Detection and Quantitation

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