Biological phosphorus release and uptake under alternating anaerobic and anoxic conditions in a fixed-film reactor

Kerrn-Jespersen, Jens Peter; Henze, Mogens; Strube, Rune

doi:10.1016/0043-1354(94)90215-1

Cited by 67 publications

(29 citation statements)

References 5 publications

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“…Although little work has been done on anoxic respiration using nitrate as a terminal electron acceptor in Acinetobacter, the inhibitory effect of NO, on ' anaerobic ' phosphate release in activated sludge has previously been noted (Egli & Zehnder, 1994;Kroiss & Negm, 1994). In mixed cultures, such inhibition is probably a result of competition from denitrifying polyp bacteria (Kerrn-Jespersen et al, 1994). No direct mechanism for the inhibition of phosphate release by nitrate has been clearly demonstrated, although Klebanoff (1993) found that nitrite had a bactericidal effect on E. coli, possibly through the generation of free radicals.…”

Section: Discussionmentioning

confidence: 99%

The effect of heavy metals and other environmental conditions on the anaerobic phosphate metabolism of Acinetobacter johnsonii

Boswell¹,

Dick²,

Macaskie³

1999

Microbiology

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A strain of Acinetobacter with potential for bioremediation of heavy metalcontaminated waters was isoJated from a wastewater-treatment plant operating an enhanced biological phosphate removal process. N M R and extractive methods showed that polyphosphate accumulated aerobically was degraded under anaerobic conditions both in the presence and absence of cadmium or uranium (02-05 mM). N M R showed that free phosphate was formed at the expense of polyphosphate, and an extractive technique indicated that this reaction could be stimulated by the presence of UOi+ under these conditions. Energy-dispersive X-ray microanalysis demonstrated that only cadmium could enter the cells, and co-localized with intra-cellular granules containing phosphate and other divalent metals. The effects of other environmental parameters on the anaerobic phosphate metabolism were also investigated. Between pH 5 5 and 8.0, phosphate release increased with increasing pH. Between 4 "C and 37 "C, phosphate release increased with increasing temperature. The presence o f nitrate a t concentrations of 10 mM and above inhibited anoxic phosphate release, but supplying tungstate in the growth medium prior to anoxic incubation reduced the production o f active nitrate reductase and atleviated this effect.

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Section: Discussionmentioning

confidence: 99%

The effect of heavy metals and other environmental conditions on the anaerobic phosphate metabolism of Acinetobacter johnsonii

Boswell¹,

Dick²,

Macaskie³

1999

Microbiology

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“…The process requires alternating anaerobic-aerobic, or anaerobic-anoxic, conditions to enrich the polyphosphate-accumulating organisms (PAOs) responsible for P removal (Kerrn-Jespersen et al, 1994;Kuba et al, 1993;Smolders et al, 1995;Wentzel et al, 1988). According to the EBPR model (Smolders et al, 1995), under anaerobic conditions, PAOs take up volatile fatty acids (VFAs) and store them intracellularly in the form of polyhydroxyalkanoates (PHAs), with the reducing power being derived from the glycolysis of glycogen and energy partly from glycogen utilization but mostly from the hydrolysis of intracellularly stored polyphosphate (polyP).…”

Section: Introductionmentioning

confidence: 97%

Identification and comparison of aerobic and denitrifying polyphosphate‐accumulating organisms

Zeng

Saunders

Yuan

et al. 2003

Biotech & Bioengineering

160

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Two laboratory-scale sequencing batch reactors (SBRs) were operated for enhanced biological phosphorus removal (EBPR) in alternating anaerobic-aerobic or alternating anaerobic-anoxic modes, respectively. Polyphosphate-accumulating organisms (PAOs) were enriched in the anaerobic-aerobic SBR and denitrifying PAOs (DPAOs) were enriched in the anaerobic-aerobic SBR. Fluorescence in situ hybridization (FISH) demonstrated that the well-known PAO, "Candidatus Accumulibacter phosphatis" was abundant in both SBRs, and post-FISH chemical staining with 4,6-diamidino-2-phenylindol (DAPI) confirmed that they accumulated polyphosphate. When the anaerobic-anoxic SBR enriched for DPAOs was converted to anaerobic-aerobic operation, aerobic uptake of phosphorus by the resident microbial community occurred immediately. However, when the anaerobic-aerobic SBR enriched for PAOs was exposed to one cycle with anoxic rather than aerobic conditions, a 5-h lag period elapsed before phosphorus uptake proceeded. This anoxic phosphorus-uptake lag phase was not observed in the subsequent anaerobic-aerobic cycle. These results demonstrate that the PAOs that dominated the anaerobic-aerobic SBR biomass were the same organisms as the DPAOs enriched under anaerobic-anoxic conditions.

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“…The capacity of DNPAOs to utilize either oxygen or nitrate as the terminal electron acceptor allows for the simultaneous nitrogen and phosphorus removal from wastewater, reducing TOC and aeration demand (Kerrn-Jespersen et al, 1994;Kuba et al, 1993). Additionally, the utilization of nitrate rather than oxygen leads to a lower cell yield, resulting in a lower sludge production (Kuba et al, 1994).…”

Section: Introductionmentioning

confidence: 99%

Sequencing batch membrane biofilm reactor for simultaneous nitrogen and phosphorus removal: Novel application of membrane‐aerated biofilm

Terada

Yamamoto

Tsuneda

et al. 2006

Biotech & Bioengineering

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A sequencing batch membrane biofilm reactor (SBMBfR) was developed for simultaneous carbon, nitrogen, and phosphorus removal from wastewater. This reactor was composed of two functional parts: (1) a gas-permeable membrane on which a nitrifying biofilm formed and (2) a bulk solution in which bacteria, mainly denitrifying polyphosphate-accumulating organisms (DNPAOs), were suspended. The reactor was operated sequentially under anaerobic condition and then under membrane aeration condition in one cycle. During the anaerobic period, organic carbon was consumed by DNPAOs; this was accompanied by phosphate release. During the subsequent membrane aeration period, nitrifying bacteria utilized oxygen supplied directly to them from the inside of the membrane. Consequently, the nitrite and nitrate products diffused into the bulk solution, where they were used by DNPAOs as electron acceptors for phosphate uptake. In a long-term sequencing batch operation, the mean removal efficiencies of total organic carbon (TOC), total nitrogen (T-N), and total phosphorus (T-P) under steady-state condition were 99%, 96%, and 90%, respectively. In addition, fluorescence in situ hybridization (FISH) clearly demonstrated the difference in bacterial community structure between the membrane biofilm and the suspended sludge: ammonia-oxidizing bacteria belonging to the Nitrosomonas group were dominant in the region adjacent to the membrane throughout the operation, and the occupation ratio of the well-known polyphosphate-accumulating organism (PAO) Candidatus "Accumulibacter phosphates" in the suspended sludge gradually increased to a maximum of 37%.

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Biological phosphorus release and uptake under alternating anaerobic and anoxic conditions in a fixed-film reactor

Cited by 67 publications

References 5 publications

The effect of heavy metals and other environmental conditions on the anaerobic phosphate metabolism of Acinetobacter johnsonii

The effect of heavy metals and other environmental conditions on the anaerobic phosphate metabolism of Acinetobacter johnsonii

Identification and comparison of aerobic and denitrifying polyphosphate‐accumulating organisms

Sequencing batch membrane biofilm reactor for simultaneous nitrogen and phosphorus removal: Novel application of membrane‐aerated biofilm

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