Biological Phosphonates: Determination by Phosphorus-31 Nuclear Magnetic Resonance

Glonek, Thomas; Henderson, Thomas O.; Hilderbrand, Richard L.; Myers, Terrell C.

doi:10.1126/science.169.3941.192

Cited by 60 publications

(19 citation statements)

References 5 publications

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“…The spectrometer used was a Bruker HFX-5 operating at 36.43 MHz for 31p (21 KGauss) (2)(3)(4) and incorporating facilities for Fourier transform signal-averaging and broadband heteronuclear 1H decoupling.…”

Section: Methodsmentioning

confidence: 99%

Phosphate Metabolism in Intact Human Erythrocytes: Determination by Phosphorus-31 Nuclear Magnetic Resonance Spectroscopy

Henderson¹,

Costello²,

Omachi³

1974

Proc. Natl. Acad. Sci. U.S.A.

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Whole human blood was examined by 31p nuclear magnetic resonance spectroscopy. Individual phosphates (a,O,-y) In this study, we have used 31P nuclear magnetic resonance spectroscopy (31P NMR) to identify phosphorus compounds in intact human erythrocytes and to record metabolic changes directly in intact erythrocytes incubated in vitro. We have detected the individual phosphate groupings (a,,By) of ATP in intact erythrocytes and have determined that there may be at least two microenvironments for this molecule. In addition, changes in 2,3-diphosphoglycerate were followed immediately after withdrawal of blood, as well as during incubation of aged cells in the presence of added inosine and pyruvate at 250.Recently, Moon and Richards (1) applied the technique of a1P NMR to the study of rabbit whole blood and hemolysates, and were able to estimate the intracellular pH of carbon monoxide-treated rabbit erythrocytes by measuring the pHinduced changes in chemical shifts of the 2-and 3-phosphates of 2,3-diphosphoglycerate. MATERIALS AND METHODSThe spectrometer used was a Bruker HFX-5 operating at 36.43 MHz for 31p (21 KGauss) (2-4) and incorporating facilities for Fourier transform signal-averaging and broadband heteronuclear 1H decoupling.

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Section: Methodsmentioning

confidence: 99%

Phosphate Metabolism in Intact Human Erythrocytes: Determination by Phosphorus-31 Nuclear Magnetic Resonance Spectroscopy

Henderson¹,

Costello²,

Omachi³

1974

Proc. Natl. Acad. Sci. U.S.A.

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“…These model compounds were used to identify peaks for myo-inositol hexaphosphate (myo-IHP, phytate), choline P, and 2-aminotheylphosphonate. Finally, Newman and Tate (1980) identified other peaks as alkyl phosphonic esters, phospholipids, and DNA based on the literature (Glonek et al, 1970;Henderson et al, 1974;Hanlon et al, 1976). Subsequent studies used these peak assignments and built on them, with Tate and Newman (1982) adding glucose 1-phosphate and ethanolamine phosphate, and Condron et al (1990) adding teichoic acid, all based on spectra of model compounds analyzed in 1 M aqueous NaOH by R.H. Newman, as for Newman and Tate (1980).…”

Section: Introductionmentioning

confidence: 98%

Improved peak identification in 31P-NMR spectra of environmental samples with a standardized method and peak library

2015

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“…31p_NMR is currently the only technique capable of resolving the abundance of phosphonates in natural sediment samples (Glonek et al, 1970). Differential hydrolysis, the only other method that has been proposed for phosphonate quantification (Aalbers & Bieber, 1968), is not sensitive enough for analysis of sediment extracts (see Appendix C-1).…”

Section: What Is the Nature Of The "Residual" Lipid P?mentioning

confidence: 99%

Organic phosphorus in marine sediments : chemical structure, diagenetic alteration, and mechanisms of preservation

Laarkamp¹

2000

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Phosphorus, an essential nutrient, is removed from the oceans only through burial with marine sediments. Organic phosphorus (Po,g) constitutes an important fraction (ca. 25%) oftotal-P in marine sediments. However, given the inherent lability of primary Po,g biochemicals, it is a puzzle that any Pm, is preserved in marine sediments. The goal of this thesis was to address this apparent paradox by linking bulk and molecular-level Po" information.A newly-developed sequential extraction method, which isolates sedimentary Po" reservoirs based on solubility, was used in concert with 3lp nuclear magnetic resonance spectroscopy (31p_NMR) to quantify Po,g functional group concentrations. The coupled extraction/ 31 P-NMR method was applied to three sediment cores from the Santa Barbara Basin, and the first-ever high-resolution depth profiles of molecular-level Po,g distribution during diagenesis were generated.These depth profiles were used to consider regulation of Pm, distribution by biomass abundance, chemical structure, and physical protection mechanisms. Biomass cannot account for more than a few percent of sedimentary Po,g. No evidence for direct structural control on remineralization of Po,g was found. Instead, sorptive protection appears to be an important mechanism for Po,g preservation, and structure may act as a secondary control due to preferential sorption of specific Pm, compound classes.

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Biological Phosphonates: Determination by Phosphorus-31 Nuclear Magnetic Resonance

Cited by 60 publications

References 5 publications

Phosphate Metabolism in Intact Human Erythrocytes: Determination by Phosphorus-31 Nuclear Magnetic Resonance Spectroscopy

Phosphate Metabolism in Intact Human Erythrocytes: Determination by Phosphorus-31 Nuclear Magnetic Resonance Spectroscopy

Improved peak identification in 31P-NMR spectra of environmental samples with a standardized method and peak library

Organic phosphorus in marine sediments : chemical structure, diagenetic alteration, and mechanisms of preservation

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