Biological parameters of Trichostrongylus colubriformis in Meriones unguiculatus

Ziam, Hocine; Pandey, Vijay; Darwiche, J; Losson, B.; Kumar, V.

doi:10.1016/s0304-4017(98)00255-6

Cited by 9 publications

(8 citation statements)

References 33 publications

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“…Jirds (Meriones unguiculatus) have been used as the experimental host of this nematode (Panitz and Shum 1981;Court and Lees 1985). In our recent study, M. unguiculatus was found to be a fascinating host model and, in terms of larval development and the course of infection, displayed conditions similar to those observed during infections in sheep (Ziam et al 1999).…”

Section: Introductionmentioning

confidence: 72%

“…It has also been shown that humoral IgG from immune hosts reduces the in vitro production of eggs by females of T. colubriformis (Bone and Klesius 1986). It has been reported that T. colubriformis normally inhabits the small intestine of jirds (Panitz and Shum 1981;Ziam et al 1999). In the present study, adult T. colubriformis were harvested from the small as well as the large intestine of both single-infection and challengeinfection groups.…”

Section: Discussionmentioning

confidence: 99%

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Drug-abbreviated infections of Trichostrongylus colubriformis and development of immunity in jirds (Meriones unguiculatus)

Ziam¹,

Pandey²,

Elegbe³

et al. 2000

Parasitol Res

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The objective of this study was to examine the development and the duration of immunity achieved with drug-abbreviated infections of Trichostrongylus colubriformis in jirds (Meriones unguiculatus). Jirds were primarily infected either by trickle infection with 6 x 100 infective larvae (L3) of T. colubriformis at 3-day intervals or by a single infection with 600 L3. On day 35 post-infection, one batch of jirds from each group was autopsied; the others were treated with oxfendazole at a dose of 5 mg/kg and were challenged with 1,000 L3 on either day 7 or day 42 post-treatment. All jirds were autopsied at 17 days post-challenge. Trickle infection resulted in lower levels of egg production during the primary infection period. The systemic IgM and IgG antibody response was significantly stronger in trickle- and single-infected groups as compared with the negative control group (P < 0.01-P < 0.05). Significantly higher levels of intestinal IgA were demonstrated in trickle- and single-infected groups than in the negative control group (P < 0.01). Numbers of mucosal mast cells increased following infection, but this was not dependent on the type of immunisation. After challenge the extent of worm reduction was greater in trickle-infected than in single-infected subgroups. The IgM and IgG response was significantly stronger in challenged subgroups as compared with negative control subgroups (P < 0.01). However, the IgG response was weaker in control challenged subgroups than in challenged subgroups (P < 0.01). There was a negative correlation between the IgG response and the worm burden after the second challenge (r = -0.73). The acquired immunity to T. colubriformis infection in jirds developed within 5 weeks of primary infection. The level of immunity was higher after trickle infection than after single infection. Furthermore, the immunity persisted for at least 6 weeks after oxfendazole treatment in the absence of a worm burden and larval intake, which is very similar to the situation in domestic ruminant hosts.

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Section: Introductionmentioning

confidence: 72%

Section: Discussionmentioning

confidence: 99%

Drug-abbreviated infections of Trichostrongylus colubriformis and development of immunity in jirds (Meriones unguiculatus)

Ziam¹,

Pandey²,

Elegbe³

et al. 2000

Parasitol Res

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“…It has been reported that both T. colubriformis and T. vitrinus are confined only in the small intestine, and the majority of the adult worms are localized in the first part of the intestine (Taylor and Kilpatrick 1980;Beveridge et al 1989;Wagland et al 1996;Ziam et al 1999). In our work, we discovered more T. colubriformis in the ileum and jejunum rather than in the duodenum (53.78% individualsTables 3 and 4), T. vitrinus was found in the duodenum in 93.33% of infected lambs and in 90% of cases, T. vitrinus was found in the jejunum and ileum (Tables 3, 4).…”

Section: Resultsmentioning

confidence: 99%

Linear distribution of nematodes in the gastrointestinal tract of tracer lambs

et al. 2008

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Forty-eight tracer lambs were killed in 2004-2007. The abomasum, duodenum, small intestine (jejunum and ileum), colon and caecum were collected and processed for parasites enumeration and identification-mucosal scrapings of both abomasums and intestines were digested. Out of 48 gastrointestinal tracts examined, all were found to be positive for nematode infection. Seventeen species of gastrointestinal nematodes were recovered: Bunostomum trigonocephalum, Cooperia curticei, Haemonchus contortus, Chabertia ovina, Nematodirus battus, Nematodirus filicollis, Oesophagostomum venulosum, Teladorsagia circumcincta, Trichostrongylus axei, Trichostrongylus colubriformis, Trichostrongylus vitrinus, Strongyloides papillosus, Trichuris ovis, Trichuris globulosa, Trichuris skrjabini and Skrjabinema ovis. All species were searched for in the entire gastrointestinal tract. Six species of nematodes were recovered from abnormal sites, naturally in small numbers of lambs as well as in small amounts: Nematodirus battus in the abomasums (6.67% of lambs), N. filicollis in the caecum and in the colon (%4 and 8%, respectively), T. axei in the colon (9.52%), T. colubriformis in the colon (13.89%), T. vitrinus in the caecum (16.67%), in the colon (20.00%) and in the abomasum (3.33%). T. ovis was found in one case in the small intestine.

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“…Many studies have been conducted using gerbils ( Meriones unguiculatus ), and this experimental model has demonstrated much promise regarding reversion of resistance (Molento & Prichard, 1999), anthelmintic evaluations (Kates & Thompson, 1967) and investigations of new anti-parasitic compounds (Ribeiro et al ., 2013). Gerbils are susceptible to infections caused by several nematodes, including Strongyloides stercoralis (Nolan et al ., 1993), Strongyloides venezuelensis , Nippostrongylus brasiliensis (Horii et al ., 1993), Trichostrongylus colubriformis (Conder et al ., 1991; Ziam et al ., 1999), H. contortus (Conder et al ., 1990) and Ostertagia circumcincta (Teladorsagia circumcincta) (Court et al ., 1988).…”

Section: Introductionmentioning

confidence: 99%

Meriones unguiculatus infected by Haemonchus contortus: evaluation of different experimental protocols

et al. 2018

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Many important studies on resistance reversion, anthelmintic efficacy and, especially, new molecules with antiparasitic effects are performed in laboratories using gerbils (Meriones unguiculatus) as the experimental model. This study aimed to evaluate the use of corticosteroids (dexamethasone and methylprednisolone acetate) in gerbils experimentally infected with different doses of infective larvae (sheathed or exsheathed) of Haemonchus contortus. In the first experiment, 28 gerbils were divided into seven groups infected by 2-6 × 103 larvae, with or without immunosuppression using corticosteroids. In the second experiment, eight gerbils were divided into two groups infected by 2 × 103 sheathed or exsheathed larvae. For the third assay, seven immunosuppressed gerbils were infected with 2 × 103 sheathed larvae and were killed 15 days post infection (PI). The highest number of parasites was recovered from methylprednisolone-immunosuppressed animals. We observed red and white blood cell alterations and biochemical parameters in infected animals that had undergone immunosuppression with methylprednisolone. We highlight that in the first and second experiments a satisfactory number of worms was recovered using sheathed larvae and immunocompetent animals. When exsheathed larvae were used, the number of worms recovered was unsatisfactory. A considerable larval burden was recovered from immunosuppressed gerbils 15 days PI, and body weight did not influence establishment of larvae.

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Biological parameters of Trichostrongylus colubriformis in Meriones unguiculatus

Cited by 9 publications

References 33 publications

Drug-abbreviated infections of Trichostrongylus colubriformis and development of immunity in jirds (Meriones unguiculatus)

Drug-abbreviated infections of Trichostrongylus colubriformis and development of immunity in jirds (Meriones unguiculatus)

Linear distribution of nematodes in the gastrointestinal tract of tracer lambs

Meriones unguiculatus infected by Haemonchus contortus: evaluation of different experimental protocols

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