Biological occupational allergy: Protein microarray for the study of laboratory animal allergy (LAA)

D’Ovidio, Maria Concetta; Wirz, Annarita; Zennaro, Danila; Massari, Stefania; Melis, Paola; Peri, Vittoria M; Rafaiani, Chiara; Riviello, M. Cristina; Mari, Adriano

doi:10.3934/publichealth.2018.4.352

Cited by 6 publications

(4 citation statements)

References 43 publications

(42 reference statements)

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“…Exposure to biocontaminants in animal facilities in USA and in the United Kingdom has received great attention [16][17][18][19] as demonstrated by the NIOSH publication in 1998 [20], where the so-called LAA (Laboratory Animal Allergy) was considered an occupational risk, and by Gordon in 2001 [21]. Various papers have been published in scientific literature both as a review [22][23][24] and experimental studies [25][26][27] while in Italy the topic of LAA is not studied carefully yet.…”

Section: Introductionmentioning

confidence: 99%

How Working Tasks Influence Biocontamination in an Animal Facility

et al. 2019

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The exposure to biocontaminants in animal facilities represents a risk for developing infectious, allergic and toxic diseases. The aim of this study was to determine what factors could be associated with a high level of exposure to biological agents through the measure and characterization of airborne fungi, bacteria, endotoxin, (1,3)-β-d-glucan and animal allergens. Airborne microorganisms were collected with an air sampler and identified by microscopic and biochemical methods. Endotoxin, (1,3)-β-d-glucan, Mus m 1, Rat n 1, Can f 1, Fel d 1, Equ c 4 allergens were detected on inhalable dust samples by Kinetic LAL, Glucatell, and ELISA assays, respectively. Our data evidenced that changing cages is a determinant factor in increasing the concentration of the airborne biocontaminants; the preparation of bedding and distribution of feed, performed in the storage area, is another critical working task in terms of exposure to endotoxins (210.7 EU/m3) and (1,3)-β-d-glucans (4.3 ng/m3). The highest concentration of Mus m 1 allergen (61.5 ng/m3) was observed in the dirty washing area. The detection of expositive peaks at risk of sensitization (>2 μg/g) by Fel d 1 in animal rooms shows passive transport by operators themselves, highlighting their role as vehicle between occupational and living environments.

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Section: Introductionmentioning

confidence: 99%

How Working Tasks Influence Biocontamination in an Animal Facility

et al. 2019

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“…Numerous categories of workers may be exposed to several biological, chemical, and physical agents that may induce and/or exacerbate allergic diseases in sensitized individuals [59][60][61][62][63][64][65][66][67].…”

Section: Pollen Allergy and Occupational Healthmentioning

confidence: 99%

New Developments in Climate Change, Air Pollution, Pollen Allergy, and Interaction with SARS-CoV-2

et al. 2023

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In recent years, the environmental impacts of climate change have become increasingly evident. Extreme meteorological events are influenced by climate change, which also alter the magnitude and pattern of precipitations and winds. Climate change can have a particularly negative impact on respiratory health, which can lead to the emergence of asthma and allergic respiratory illnesses. Pollen is one of the main components of the atmospheric bioaerosol and is able to induce allergic symptoms in certain subjects. Climate change affects the onset, length, and severity of the pollen season, with effects on pollen allergy. Higher levels of carbon dioxide (CO2) can lead to enhanced photosynthesis and a higher pollen production in plants. Pollen grains can also interact with air pollutants and be affected by thunderstorms and other extreme events, exacerbating the insurgence of respiratory diseases such as allergic rhinitis and asthma. The consequences of climate change might also favor the spreading of pandemics, such as the COVID-19 one.

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“…Laboratory animal allergy (LAA) is a relevant occupational health hazard for workers attending veterinary or research animal facilities. It was estimated that as many as 44% of personnel operating in contact with laboratory animals report symptoms related to occupational asthma or allergic rhinoconjunctivitis [ 1 , 2 ].…”

Section: Introductionmentioning

confidence: 99%

Component-Resolved Diagnosis Based on a Recombinant Variant of Mus m 1 Lipocalin Allergen

Ferrari

Breda

Spisni

et al. 2023

IJMS

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Exposure to the Mus m 1 aeroallergen is a significant risk factor for laboratory animal allergy. This allergen, primarily expressed in mouse urine where it is characterized by a marked and dynamic polymorphism, is also present in epithelium and dander. Considering the relevance of sequence/structure assessment in protein antigenic reactivity, we compared the sequence of the variant Mus m 1.0102 to other members of the Mus m 1 allergen, and used Discotope 2.0 to predict conformational epitopes based on its 3D-structure. Conventional diagnosis of mouse allergy is based on serum IgE testing, using an epithelial extract as the antigen source. Given the heterogeneous and variable composition of extracts, we developed an indirect ELISA assay based on the recombinant component Mus m 1.0102. The assay performed with adequate precision and reasonable diagnostic accuracy (AUC = 0.87) compared to a routine clinical diagnostic test that exploits the native allergen. Recombinant Mus m 1.0102 turned out to be a valuable tool to study the fine epitope mapping of specific IgE reactivity to the major allergen responsible for mouse allergy. We believe that advancing in its functional characterization will lead to the standardization of murine lipocalins and to the development of allergen-specific immunotherapy.

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Biological occupational allergy: Protein microarray for the study of laboratory animal allergy (LAA)

Cited by 6 publications

References 43 publications

How Working Tasks Influence Biocontamination in an Animal Facility

How Working Tasks Influence Biocontamination in an Animal Facility

New Developments in Climate Change, Air Pollution, Pollen Allergy, and Interaction with SARS-CoV-2

Component-Resolved Diagnosis Based on a Recombinant Variant of Mus m 1 Lipocalin Allergen

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