Biological interactions between soil saprotrophic fungi and Ascaris suum eggs

Błaszkowska, Joanna; Wójcik, Anna; Kurnatowski, Piotr; Szwabe, Katarzyna

doi:10.1016/j.vetpar.2013.02.029

Cited by 10 publications

(4 citation statements)

References 27 publications

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“…The collected eggs were centrifuged in distilled water (3× at 1000 rpm) for 3 min. A. suum eggs were incubated in distilled water containing 0.05% formalin, 0.05% streptomycin sulfate, and 0.01% chloramphenicol [46,47].…”

Section: Ascaris Suum Eggsmentioning

confidence: 99%

Effect of Selected Entomopathogenic Fungal Species on Embryonic Development of Ascaris suum (Nematoda)

Mazurkiewicz-Zapałowicz,

Pilarczyk,

Kołodziejczyk

et al. 2023

Animals

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The aim of the study was to evaluate the potential of using five selected species of entomopathogenic fungi (Beauveria bassiana, B. brongniartii, Conidiobolus coronatus, Isaria fumosorosea, and Metarhizium robertsii) in the bioregulation of the dispersive stages of the parasitic nematode—Ascaris suum. Experimental cultures of each of the selected entomopathogenic fungi, as well as a control culture without fungi, were incubated with A. suum eggs at 26 °C for 28 days. Development of the A. suum eggs was observed using a light microscope on the 7th, 14th, 21st, and 28th days of incubation. The API-ZYM® test was used to determine, semiquantitatively, the activity of 19 hydrolytic enzymes from the entomopathogenic fungi. The cytotoxicity of the fungi was determined using tetrazole salt MTT. It was found that none of the five tested strains of entomopathogenic fungi showed an ovicidal effect, and none of them colonized the A. suum egg shells. However, ovistatic activity was observed mainly until the 14th day of incubation by I. fumosorosea, M. robertsii, and B. bassiana. In the MTT test, M. robertsii showed moderate cytotoxicity, while the other species showed low cytotoxicity. Among the strains tested, I. fumosorosea showed the highest spectrum of hydrolase production (13 out of 19 enzymes gave a positive reaction from 3 to 5; 20–40 nM or more). The absence of morphological changes in the A. suum egg shells suggests that the antagonistic effect of the studied entomopathogenic fungi may be due to their cytotoxicity, associated with the production of secondary metabolites—toxins (M. robertsii) and enzymatic activity (I. fumosorosea).

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Section: Ascaris Suum Eggsmentioning

confidence: 99%

Effect of Selected Entomopathogenic Fungal Species on Embryonic Development of Ascaris suum (Nematoda)

Mazurkiewicz-Zapałowicz,

Pilarczyk,

Kołodziejczyk

et al. 2023

Animals

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“…For example, the pore-forming crystal protein Cry5B, derived from Bacillus thuringiensis, exhibits considerable anthelmintic activity, killing Ascaris in vitro and in vivo [16]. Interestingly, saprotrophic fungal strains, such as Aspergillus fumigatus, can inhibit larval development and viability of A. suum eggs [17]. Though these are soil-dwelling organisms, their nematicidal activity exemplifies direct microbial threats to Ascaris which may also be posed by intestinal microbes.…”

Section: Relevance Of Ascaris-microbiota-host Cell Interactionsmentioning

confidence: 99%

“…Whilst the impact of Ascaris infection on the host mycobiome has not been addressed so far, mutual effects were observed in vitro. These include the demonstration of antifungal or anthelmintic properties of cecropins released by Ascarids and Aspergillus products, respectively [76,101], as well as the impaired embryonic development of Ascaris eggs exposed to saprotrophic soil fungi [17,102].…”

Section: Parasite-fungus Interactionsmentioning

confidence: 99%

Trilateral Relationship: Ascaris, Microbiota, and Host Cells

Midha

Ebner

Schlosser-Brandenburg

et al. 2021

Trends in Parasitology

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“…llo evolutivo de los huevos de Ascaris suum 48 , y más recientemente, la evaluación de los efectos ovistáticos u ovicidas de hongos filamentosos asilados del suelo sobre huevos de A. suum 49 .…”

Section: Ensayos Sobre Huevos De Nematodos Y Purificación De Enzimas unclassified

Interacción biológica de las enzimas producidas por hongos nematófagos saprófitos de suelo, contra huevos de Toxocara canis

Bojanich¹

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Los hongos nematófagos son enemigos naturales de los nematodos ya que pueden atacar nematodos vivos o sus huevos y utilizarlos como nutrientes. Para ello se valen de adaptaciones morfológicas de su micelio. El objetivo de este trabajo de investigación fue determinar la interacción biológica in vitro de hongos saprófitos de suelo con huevos de T. canis, observando la actividad mecánica y determinando la actividad enzimática entre los hongos saprófitos y los huevo de T. canis. Para ello se aislaron 40 géneros y 69 especies fúngicas saprófitas de suelos de plazas y parques de la ciudad de Corrientes. La frecuencia de huevos de T. canis en suelos fue de 13,43%. Para realizar los ensayos de interacción se eligieron los hongos, Ch. indicum, Ch. keratinophylum, C. lunata y T. ajelloi, obedeciendo esa elección a: frecuencia de recuperación en los suelos, antecedentes de antagonismo con huevos de nematodos tanto positivo como negativo y actividad quitinolítica. Como resultado de la interacción aparecen Ch. indicum, Ch. keratinophylum y C. lunata presentando un efecto ovicida alto (72,7, 74 y 78% de huevos afectados respectivamente); en tanto que T. ajelloi, con 18% de actividad. Las interacciones observadas al microscopio óptico y al Microscopio Electrónico de Barrido sobre los huevos de T. canis fueron: hifas rodeando y penetrando los huevos, cambios en la membrana del huevo, cubierta adelgazada con aumento de la permeabilidad y sin la trama fosetada característica, y larvas vacuoladas. Las hifas pueden penetrar y desarrollarse en el interior del huevo. Los enfrentamientos extractos enzimáticos crudos– huevos de T. canis revelaron las siguientes alteraciones: cambios en las características de la cubierta, entre ellos, adelgazamiento y permeabilidad en exceso, larvas L1 sin movimiento, desorganización interna y vacuolización del embrión.

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Biological interactions between soil saprotrophic fungi and Ascaris suum eggs

Cited by 10 publications

References 27 publications

Effect of Selected Entomopathogenic Fungal Species on Embryonic Development of Ascaris suum (Nematoda)

Effect of Selected Entomopathogenic Fungal Species on Embryonic Development of Ascaris suum (Nematoda)

Trilateral Relationship: Ascaris, Microbiota, and Host Cells

Interacción biológica de las enzimas producidas por hongos nematófagos saprófitos de suelo, contra huevos de Toxocara canis

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