Biological incorporation of human acellular dermal matrix used in Achilles tendon repair

Abstract

Human acellular dermal matrices (ADMs) are used successfully in a variety of procedures, including sports medicine related, wound repair, and breast reconstructions, but the mechanism of repair is still not fully understood. An opportunity to explore this mechanism presented itself when a patient experienced a rerupture of the native tendon due to a fall that occurred 2 months after undergoing an Achilles tendon repair using Matracell treated ADM. The ADM was removed and an extensive histology analysis was performed on the tissue. Additionally, a literature review was conducted to determine the mechanism of ADM integration into the tendon structure and explore if differences in this mechanism exist for different types of human ADMS. The histology analysis demonstrated that the healing process during a tendon reconstruction procedure is similar to that of wound healing. Furthermore, the literature review showed that differences exist in the mechanism for integration among various human ADMs and that these differences may be due to variances in the methods and technologies that manufactures use to process human ADMs.

Scleral defect repair using decellularized porcine sclera in a rabbit model

“…23,30 During the decellularization processes, it is important to preserve intact unique structures and matrix components for tissue development and wound healing. [31][32][33][34][35][36][37] Here, we provide a modified method for the decellularization of porcine sclera by using cells disrupted with a salt solution, the mild detergent Triton X-100, and supernuclease. The results from histological staining, SEM, and TEM confirmed the undamaged architecture and retention of active ingredients in the ECM, suggesting that scleral collagen fibers did not suffer evident disruption.…”

“…During the decellularization processes, it is important to preserve intact unique structures and matrix components for tissue development and wound healing 31‐37 . Here, we provide a modified method for the decellularization of porcine sclera by using cells disrupted with a salt solution, the mild detergent Triton X‐100, and supernuclease.…”

Load-deformation characteristics of acellular human scalp: assessing tissue grafts from a material testing perspective

“…Moreover, it has been shown that the biomechanical properties of grafts may influence wound healing at the wound edges 7 following surgical interventions, with the scaffold’s stiffness being a strong regulator of stem cell differentiation 8 . Human acellular skin is a widely deployable graft material, which has, among others, been used for the treatment of chronic wounds 9 , congenital skin absence 10 , tendon repair 11 or cleft palate repair 5 . This given study is part of the ongoing Human Head Tissues Biomechanics project, which focuses on the mechanical description of human head tissues and their biomechanically adequate surgical replacement.…”

Rapid porcine corneal decellularization through the use of sodium N-lauroyl glutamate and supernuclease

“…31 As a genetically engineered endonuclease, benzonase has been used for decellularizing dermis and corneas. 32,33 Benzonase can quickly infiltrate into corneal stroma, efficiently degrade all forms of DNA and RNA without proteolytic activity, and also easily be removed by repeated washing. 34 Supernuclease is a homologous nuclease of benzonase.…”