Biological Imaging of HEK293 Cells Expressing PLCγ1 Using Surface-Enhanced Raman Microscopy

Lee, Sangyeop; Kim, Sungyong; Choo, Jaebum; Shin, Soon Young; Lee, Young Han; Choi, Ha Young; Ha, Seunghan; Kang, Ku; Oh, Cheol

doi:10.1021/ac061246a

Cited by 271 publications

(186 citation statements)

References 29 publications

(36 reference statements)

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“…Roughened surface noble metal nanoparticles labeled with binding affinity molecules have also been shown to dramatically increase the Raman signal of their complementary molecules once attached through the SERS mechanism (12,20,21). Conjugation of monoclonal antibodies with SERS nanoparticles for targeting and imaging of specific cancer markers in live cultured cells was also recently investigated (5). Another study presents the first in vivo application of SERS for glucose measurements in a rat (22) by s.c. implantation of functionalized SERS nanospheres.…”

Section: Discussionmentioning

confidence: 99%

“…Fluorescence imaging, in particular, has significant potential for in vivo studies but is limited by several factors (5,6), including a limited number of fluorescent molecular imaging agents available in the near infra-red (NIR) window with large spectral overlap between them, which restricts the ability to interrogate multiple targets simultaneously (multiplexing). In addition, background autofluorescence emanating from superficial tissue layers restricts the sensitivity and the depth to which fluorescence imaging can be used.…”

mentioning

confidence: 99%

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Noninvasive molecular imaging of small living subjects using Raman spectroscopy

Keren

Zavaleta

Cheng

et al. 2008

Proc. Natl. Acad. Sci. U.S.A.

558

475

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Molecular imaging of living subjects continues to rapidly evolve with bioluminescence and fluorescence strategies, in particular being frequently used for small-animal models. This article presents noninvasive deep-tissue molecular images in a living subject with the use of Raman spectroscopy. We describe a strategy for small-animal optical imaging based on Raman spectroscopy and Raman nanoparticles. Surface-enhanced Raman scattering nanoparticles and single-wall carbon nanotubes were used to demonstrate whole-body Raman imaging, nanoparticle pharmacokinetics, multiplexing, and in vivo tumor targeting, using an imaging system adapted for small-animal Raman imaging. The imaging modality reported here holds significant potential as a strategy for biomedical imaging of living subjects.nanotubes ͉ SERS nanoparticles M olecular imaging of living subjects provides the ability to study cellular and molecular processes that have the potential to impact many facets of biomedical research and clinical patient management (1-4). Imaging of small-animal models is currently possible by using positron emission tomography (PET), single photon emission computed tomography, magnetic resonance imaging, computed tomography, optical bioluminescence and fluorescence, high frequency ultrasound, and several other emerging modalities. However, no single modality currently meets the needs of high sensitivity, high spatial and temporal resolution, high multiplexing capacity, low cost, and high-throughput.Fluorescence imaging, in particular, has significant potential for in vivo studies but is limited by several factors (5, 6), including a limited number of fluorescent molecular imaging agents available in the near infra-red (NIR) window with large spectral overlap between them, which restricts the ability to interrogate multiple targets simultaneously (multiplexing). In addition, background autofluorescence emanating from superficial tissue layers restricts the sensitivity and the depth to which fluorescence imaging can be used. Moreover, rapid photobleaching of fluorescent molecules limits their useful lifetime and prevents studies of prolonged duration. Therefore, we have attempted to develop new strategies that may solve some of the limitations of fluorescence imaging in living subjects.Raman spectroscopy can differentiate the spectral fingerprint of many molecules, resulting in very high multiplexing capabilities. Narrow spectral features are easily separated from the broadband autofluorescence, because Raman is a scattering phenomenon as opposed to absorption/emission in fluorescence, and Raman active molecules are more photostable compared with fluorophores, which are rapidly photobleached. Unfortunately, the precise mechanism for photobleaching is not well understood. However, it has been linked to a transition from the excited singlet state to the excited triplet state. Photobleaching is significantly reduced for single molecules adsorbed onto metal particles because of the rapid quenching of excited electrons by the metal surface...

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Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

Noninvasive molecular imaging of small living subjects using Raman spectroscopy

Keren

Zavaleta

Cheng

et al. 2008

Proc. Natl. Acad. Sci. U.S.A.

558

475

View full text Add to dashboard Cite

show abstract

“…SERS targeted measurement and identification of several types of cancer cell has been shown, including osteosarcoma cells using AuNP SERS [303], cancerous renal cell detection [304], and Ag/Au core-shell nanoparticles conjugated with antibodies were used to image PLCγ protein in HEK293 cells [305]. Targeted SERS detection of circulating tumor cells has been shown in the presence of white blood cells [306].…”

Section: Applicationsmentioning

confidence: 99%

Raman spectroscopy: techniques and applications in the life sciences

Shipp¹,

Sinjab²,

Notingher³

2017

Adv. Opt. Photon.

255

189

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Raman spectroscopy is an increasingly popular technique in many areas including biology and medicine.It is based on Raman scattering, a phenomenon in which incident photons lose or gain energy via interactions with vibrating molecules in a sample. These energy shifts can be used to obtain information regarding molecular composition of the sample with very high accuracy. Applications of Raman spectroscopy in the life sciences have included quantification of biomolecules, hyperspectral molecular imaging of cells and tissue, medical diagnosis, and others. This review briefly presents the physical origin of Raman scattering explaining the key classical and quantum mechanical concepts. Variations of the Raman effect will also be considered, including resonance, coherent, and enhanced Raman scattering. We discuss the molecular origins of prominent bands often found in the Raman spectra of biological samples. Finally, we examine several variations of Raman spectroscopy techniques in practice, looking at their applications, strengths, and challenges. This review is intended to be a starting resource for scientists new to Raman spectroscopy, providing theoretical background and practical examples as the foundation for further study and exploration.

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“…However, Ag is considered unstable, degrades in vivo and is typically synthesized with cetrimonium bromide (CTAB), which is considered cytotoxic [282][283][284]. Additionally, it has been demonstrated that the peak intensity ratios of the Raman signal change significantly when silver aggregates are used leading to inconsistent results and poor reproducibility [276,[285][286][287].…”

Section: Surface Enhanced Raman Scattering (Sers) and Electromagneticmentioning

confidence: 99%

Controllable Cobalt Oxide/Au Hierarchically Nanostructured Electrode for Nonenzymatic Glucose Sensing

2016

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By electrodeposition and galvanic replacement reaction, we developed a facile, time-saving, cost-effective, and environmentally friendly, two-step synthesis route to obtain a controllable cobalt oxide/Au hierarchically nanostructured electrode for glucose sensing. The nanomaterials were characterized by transmission electron microscopy, scanning electron microscopy, Raman spectroscopy, energy-dispersive spectrometry, and X-ray photoelectron spectroscopy, meanwhile, the sensing performance was investigated by cyclic voltammetry and amperometric response. The results revealed that this novel electrode exhibited excellent electrocatalytic performance toward glucose oxidation, with a wide double-linear range from 0.2 μM to 20 mM and a low detection limit of 0.1 μM based on a signal-to-noise ratio of 3, which was mainly attributed to the ability of loading a small amount of Au with good electron conductivity on the surface of cobalt oxide nanosheets with large active surface area and synergistic electrocatalytic activity of Au and cobalt oxide toward glucose electrooxidation. This facile, sensitive, and selective glucose sensor is also proven to be suitable for the detection of glucose in human serum.

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Biological Imaging of HEK293 Cells Expressing PLCγ1 Using Surface-Enhanced Raman Microscopy

Cited by 271 publications

References 29 publications

Noninvasive molecular imaging of small living subjects using Raman spectroscopy

Noninvasive molecular imaging of small living subjects using Raman spectroscopy

Raman spectroscopy: techniques and applications in the life sciences

Controllable Cobalt Oxide/Au Hierarchically Nanostructured Electrode for Nonenzymatic Glucose Sensing

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