Biological fitness ofTrichoderma atrovirideduring long-term storage, after production in different culture conditions

Effects of temperature, light and incubation period on production, germination and bioactivity ofTrichoderma atroviride

“…The numbers of conidia harvested from the cultures were counted using a microscope slide haemocytometer and then were used for germination and bioactivity (inhibition) assessments as described by Daryaei et al . (). The bioactivity experiment was carried out using half strength PDA plates as it showed clear inhibition zone in dual agar culture assays.…”

“…This study was part of a programme aimed to develop new knowledge of effects of growth conditions, to increase persistence and efficiency of the key agent T. atroviride LU132, which has been commercialized and to understand the factors which may influence conidium fitness (quantity and quality) for biological control formulations of conidia that are robust (long‐surviving) and active against target plant pathogens (Daryaei ; Daryaei et al . ,b,c,d). Although the effects of culture conditions on the quantity of conidia produced of this strain have been previously studied (Steyaert et al .…”

pH and water activity in culture media affect biological control activity of Trichoderma atroviride against Rhizoctonia solani

“…Previous studies on conidium fitness (quantity and quality) of T. atroviride LU132 have shown that the optimum incubation temperature to maximise these characteristics was close to 25°C, and optimum bioactivity occurred after 15 d incubation (Daryaei et al, 2015). In the present study, the effect of culture pH and a w on conidium production and fitness as assessed by germinability and bioactivity against an important soil-borne plant pathogen, Rhizoctonia solani.…”

“…After 15 d incubation, one plate was randomly selected from each replicate or block of each conidium production treatment, and the conidia produced at the different pH and a w levels were harvested in SDW (10 ml per plate) and assessed for numbers of conidia, germination percentage and bioactivity as described by Daryaei (2014) and Daryaei et al (2015). From the pH experiment, harvested conidia from the 50 mM buffered pH series only were used for assays of germination and bioactivity.…”

“…T. atroviride LU132 has been shown to improve growth and establishment of ryegrass in the presence of R. solani (Kandula et al, 2015). Optimum temperature and incubation time as established in previous studies (Daryaei et al, 2015) were applied to test effects of pH and a w in growth medium in the present study. This information is important to inform the commercial production of BCAs, particularly those based on T. atroviride.…”