Biological Fingerprinting of Herbal Samples by Means of Liquid Chromatography

Cieśla, Łukasz

doi:10.1155/2012/532418

Cited by 29 publications

(18 citation statements)

References 43 publications

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“…Fingerprint construction is helpful to detect adulterations, to control the process of extraction, and to test the quality of a finished product. Herbal sample fingerprints are a set of characteristic chromatographic or spectroscopic signals that enable sample recognition through comparison (Ciesla 2012). While many chromatographic methods have been used for fingerprint construction Springfield et al (2005) suggested that fingerprinting using HPLC is the best method for extract characterization.…”

Section: Discussionmentioning

confidence: 99%

Larvicidal potency of selected xerophytic plant extracts on Culex pipiens (Diptera: Culicidae)

Abutaha

Galil

Al-Keridis

et al. 2018

Entomological Research

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Chemical insecticides released into the environment may have adverse biological effects. Therefore, there is a need for ecofriendly insecticides for mosquito control. Xerophytic plant extracts that may provide more ecofriendly active component were evaluated against Culex pipiens 4th instars. Plant extracts prepared using different solvents with a Soxhlet apparatus and different concentrations were tested against Culex pipiens larvae. The effects were observed at 24 h and 72 h intervals and LD50 and LD90 values determined. Chloroform (CHCl3) and ethyl acetate (EtOAc) extracts of Althaea ludwigii were the most effective against Cx. pipiens 4th instars, but were highly dependent on extract concentrations and exposure time. Results suggest that A. ludwigii extracts contain bioactive compounds, such as phenols and saponins, that may provide effective Cx. pipiens larval control. However, the extract was found to be toxic to zebrafish larvae, and may be toxic to other aquatic fauna. Further studies to determine the active components and toxicity to other fauna are needed.

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Section: Discussionmentioning

confidence: 99%

Larvicidal potency of selected xerophytic plant extracts on Culex pipiens (Diptera: Culicidae)

Abutaha

Galil

Al-Keridis

et al. 2018

Entomological Research

View full text Add to dashboard Cite

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“…It was originally developed with the use of high-performance liquid chromatography. Apart from qualitative and quantitative data, it gives also the possibility to fish out the active compounds from a myriad of compounds present in herbal samples 3 . Chemical fingerprinting has been demonstrated to be a powerful technique for the quality control of herbal medicines.…”

Section: Introductionmentioning

confidence: 99%

Untitled

2019

IJPSR

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Development of quality assessment parameters for natural products is a prominence necessity to justify their acceptability and activity. Establishment of authentic and reliable analytical methods which profile the quantitative phytochemical composition of marker constituents in multicomponent composition like extract is a challenging task. A simple, rapid, precise, and reliable HPLC method was developed for the separation and estimation of ellagic acid from Epilobium angustifolium (Canadian willow herb) extract. The estimation was carried out using Sunfire C18 column, 5μ (4.6 × 250 mm); mobile phase consisting of 0.1% orthophosphoric acid and acetonitrile; the flow rate of 1 mL/min and ultraviolet detection at 280 nm with a properly resolved having run time of 35 min. The method was validated as a final verification of method development concerning precision, linearity, accuracy, ruggedness, and robustness. The correlation coefficient (r 2) > 0.999, a method is considered to be linear as the correlation coefficient was found to be within acceptance criteria. The % RSD of peak area response due to ellagic acid in five replicate injections of standard solution was to be less than 2.0%, and system suitability parameters were passed. The % Average recovery of ellagic acid in Canadian willow Extract observed within the acceptance criterion of 98-102% indicates the accuracy of the method. The present validation proves that the HPLC-method is suitable for the determination of assay of ellagic acid from Canadian willow herb, extract at prescribed conditions.

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“…Bioassay-coupled chromatography has also found application in biological finger printing of herbal samples. 8 Herbal finger printing involves a set of characteristic chromatographic and spectroscopic signals which lead to unambiguous sample identification upon comparison with reference(s). However, since traditional chromatographic analysis could only provide qualitative and quantitative information, compounds present in low concentration which may be responsible for the biological activity of the sample may not be detected.…”

Section: Introductionmentioning

confidence: 99%

“…However, since traditional chromatographic analysis could only provide qualitative and quantitative information, compounds present in low concentration which may be responsible for the biological activity of the sample may not be detected. 8 Thus, the need to add a system which involves bioassay coupled with chromatography and spectroscopy. In the search for active compounds from highly complex sample mixtures such as in the field of natural product research, target analysis only is ineffective.…”

Section: Introductionmentioning

confidence: 99%