Biological Evaluation of Silver Nanoparticles Incorporated Into chitosan-based Membranes

Shao, Jinlong; Yu, Na; Kolwijck, Eva; Wang, B.; Tan, Kar Woon; Jansen, John A.; Walboomers, X. Frank; Yang, Fang

doi:10.2217/nnm-2017-0172

Cited by 43 publications

(32 citation statements)

References 46 publications

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“…Chitosanbased membranes supplemented with 5, 10 or 15% w/w of silver nanoparticles showed good antimicrobial activity against gram-positive and gram-negative bacteria 43) . A similar phenomenon has been reported for silver supplementation to chitosan membranes, which inhibited Porphyromonas gingivalis and Fusobacterium nucleatum growth in a dose-dependent way 17) . However, silver nanoparticles have an important cytotoxicity on human cells limiting their application on clinical practice 19) .…”

Section: Discussionsupporting

confidence: 80%

“…Chemical cross-linkers, like glutaraldehyde, can fix the membranes for increased stability and mechanical strength 14) . To confer antimicrobial properties, chitosan membranes have been supplemented with several compounds with antimicrobial properties, such as antibiotics, silver sulfasalazine, chlorhexidine, and silver nanoparticles [15][16][17] . However, chlorhexidine and silver nanoparticles have the disadvantage of being highly cytotoxic, which limits their usage in clinical practice 18,19) .…”

Section: Introductionmentioning

confidence: 99%

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Antimicrobial potential of bismuth lipophilic nanoparticles embedded into chitosan-based membrane

et al. 2019

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The objective of this work was to analyze the antimicrobial and antibiofilm activities of bismuth lipophilic nanoparticles (BisBAL NPs) incorporated into chitosan-based membranes. Chitosan-based membranes were homogeneously embedded with BisBAL NPs, confirming the bismuth presence by scanning electron microscopy. The tensile strength of chitosan-based membrane alone or with BisBAL NPs showed similar results as elongation, suggesting that BisBAL NP addition did not affect membrane mechanical properties. Chitosan-based membranes complemented with 100 µM of BisBAL NPs caused a complete inhibition of biofilm formation and a 90-98% growth inhibition of six different oral pathogens. Cytotoxicity studies revealed that 80% of human gingival fibroblasts were viable after a 24-h exposure to the chitosan-based membrane with 100 µM of BisBAL NPs and collagen. Altogether, we conclude that the biological properties of chitosan-based membranes supplemented with BisBAL NPs could be a very interesting option for tissue regeneration.

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Section: Discussionsupporting

confidence: 80%

Section: Introductionmentioning

confidence: 99%

Antimicrobial potential of bismuth lipophilic nanoparticles embedded into chitosan-based membrane

et al. 2019

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“…Moreover, AgNPs have a much lower tendency to induce bacterial resistance compared with classical antibiotics (Sondi and Salopeksondi, 2004). Shao et al (2017) fabricated a chitosan-based membrane blended with AgNPs. The results of antibacterial property evaluation revealed that AgNPs could have sustained antibacterial properties against Porphyromonas gingivalis and Fusobacterium nucleatum in a dose-dependent manner.…”

Section: Nano-composite Electrospun Fibers In Periodontal Regenerationmentioning

confidence: 99%

Advance of Nano-Composite Electrospun Fibers in Periodontal Regeneration

Lin

2019

Front. Chem.

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Periodontitis is considered to be the main cause of tooth loss, which affects about 15% of the adult population around the world. Scaling and root-planning are the conventional treatments utilized to remove the contaminated tissue and bacteria, but eventually lead to the formation of a poor connection—long junctional epithelium. Therefore, regenerative therapies, such as guided tissue/bone regeneration (GTR/GBR) for periodontal regeneration have been attempted. GTR membranes, acting as scaffolds, create three-dimensional (3D) environment for the guiding of cell attachment, proliferation and differentiation, and play a significant role in periodontal regeneration. Nano-composite scaffolds based on electrospun nanofibers have gained great attention due to their ability to emulate natural extracellular matrix (ECM) that affects cell survival, attachment and reorganization. Promoted protein absorption, cellular reactions, activation of specific gene expression and intracellular signaling, and high surface area to volume ratio are also important properties of nanofibrous scaffolds. Moreover, several bioactive components, such as bioceramics and functional polymers can be easily blended into nanofibrous matrixes to regulate the physical-chemical-biological properties and regeneration abilities. Simultaneously, functional growth factors, proteins and drugs are also incorporated to regulate cellular reactions and even modify the local inflammatory microenvironment, which benefit periodontal regeneration and functional restoration. Herein, the progress of nano-composite electrospun fibers for periodontal regeneration is reviewed, including fabrication methods, compound types and processes, and surface modifications, etc. Significant proof-of-concept examples are utilized to illustrate the results of material characteristics, cellular interactions and periodontal regenerations. Finally, the existing limitations of nano-composite electrospun fibers and the development tendencies in future are also discussed.

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“…The nanocomposite material based on CsNPs and AgNPs, the Cs‐AgNPs, has been investigated in biomedical and pharmaceutical areas because of their strong antimicrobial properties against pathogenic microorganisms and multidrug‐resistant bacteria. [ 18,158–162 ]…”

Section: Chitosan–silver Nanoparticles (Cs‐agnps)mentioning

confidence: 99%

New Perspectives of Using Chitosan, Silver, and Chitosan–Silver Nanoparticles against Multidrug‐Resistant Bacteria

Polinarski

Beal

Silva

et al. 2021

Part & Part Syst Charact

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materials are typically developed at a critical length of under 100 nm. However, other phenomena, such as transparency and stable dispersion, can occasionally extend the upper limit, and the use of the prefix "nano" is accepted for dimensions smaller than 500 nm. [2] Two relevant factors differentiate nanomaterials from macroscopic materials: first, the quantum effect that can promote changes in physical properties, such as color and electrical conductivity, and second, the surface effect related to the increased surface area to volume ratio that favors physical and chemical interactions between atoms and the surrounding environment. [3] NPs are of the same order of magnitude as antibodies, membrane receptors, nucleic acids, proteins, and other biomolecules, thus representing a potential material to be used in medicine for imaging applications, diagnosis, therapies, or in medical devices. [4] The small size and large surface area of NPs facilitate their permeation through cell membranes and enhance their biological activity. [5] Microbial colonization of medical devices leads to the formation of microbial or fungal biofilms, which are complex assemblages of microbial cells associated with a surface and incorporated into an extracellular matrix. [6] Biofilms are critical clinical issues because they culminate in the pathogenesis of numerous bacterial infections that are difficult to treat and effectively eradicate with antibiotics. [7] Biofilm formation can occur in three stages: attachment, maturation, and dispersion. The attachment step can be further categorized in two-stage processes: initial reversible attachment and irreversible attachment, in which the attached biofilm can tolerate strong shear forces. [8] The bacteria deposition is mediated by sedimentation, Brownian motion, and hydrodynamic forces, whereas adhesion is governed by Van der Waals, acid-base, hydrophobic, and electrostatic interaction forces. [9] Attachment is the most crucial phase to prevent bacterial adhesion because the antimicrobial action becomes less effective after biofilm formation. [10] Medical devices commonly affected by microbial contamination and biofilm formation are catheters, probes, and wound dressings. Catheters are used in patients to administer fluids, blood products, and parenteral nutrition. [6] Most nosocomial infections in intensive care units (ICUs) are associated with the insertion and maintenance of central venous catheters Nanomaterials with antimicrobial activity are promising alternatives to overcome microbial resistance in medical devices. Catheters, probes, and wound dressings are among the medical devices mostly affected by microbial contamination and the formation of polymicrobial biofilms. Nanoparticles (NPs) derived from natural sources, such as chitosan nanoparticles (CsNPs), and metal-based nanoparticles, including silver nanoparticles (AgNPs), are receiving increased interest in nanomedicine. CsNPs have been widely explored as a coating material and antimicrobial agent. AgNPs have a strong antimicrobi...

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Biological Evaluation of Silver Nanoparticles Incorporated Into chitosan-based Membranes

Abstract: The antibacterial effect, combined with the findings on cyto- and biocompatibility warrants further investigation to the usefulness of chitosan/poly(ethylene oxide) membranes with silver nanoparticles, for clinical applications like guided tissue regeneration.

Cited by 43 publications

References 46 publications

Antimicrobial potential of bismuth lipophilic nanoparticles embedded into chitosan-based membrane

Antimicrobial potential of bismuth lipophilic nanoparticles embedded into chitosan-based membrane

Advance of Nano-Composite Electrospun Fibers in Periodontal Regeneration

New Perspectives of Using Chitosan, Silver, and Chitosan–Silver Nanoparticles against Multidrug‐Resistant Bacteria

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