Biological Evaluation of Chitosan Nanofiber Membrane for Guided Bone Regeneration

Shin, Seung‐Yun; Park, Ho Nam; Kim, Kyoung Hwa; Lee, Myung Hee; Choi, Youngsok; Park, Yoon Jeong; Lee, Yong-Moo; Ku, Young; Rhyu, In‐Chul; Han, Soo Boo; Lee, Seung Jin; Chung, Chong Pyoung

doi:10.1902/jop.2005.76.10.1778

Cited by 160 publications

(102 citation statements)

References 32 publications

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“…Hence, there is a need for new method to prevent infection of GBR/GTR membranes and regenerating alveolar bone. Electrospun chitosan and other chitosan membrane materials have been advocated for GBR/GTR barrier membranes [1][2][3]. Chitosan is advantageous because it has been reported to stimulate the healing of dental pulp wounds, [4] is osteogenic, and has been shown to inhibit lippopolysaccharide (LPS)-induced monocyte activation [5,6].…”

Section: Introductionmentioning

confidence: 99%

Antimicrobial Activity of Minocycline-Loaded Genipin-Crosslinked Nano-Fibrous Chitosan Mats for Guided Tissue Regeneration

Norowski¹,

Babu²,

Adatrow³

et al. 2012

JBNB

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Antimicrobial delivery has been advocated for guided tissue regeneration (GTR) or guided bone regeneration (GBR) therapies involving patients with aggressive or unresolved periodontitis/peri-implantitis. Electrospun chitosan membranes demonstrate several advantages over traditional GTR barrier membranes because they stimulate healing, mimic the topology of the extracellular matrix, and allow for diffusion of nutrients and wastes into/out of the graft site, and were shown to stimulate bone formation in a rabbit calvarial critical-size defect model. Previously, we have shown improvements in mechanical properties and degradation kinetics by crosslinking electrospun membranes with 5 mM or 10 mM genipin. We have also demonstrated the ability of elecrospun chitosan membranes to inhibit lippopolysaccharide (LPS)-induced monocyte activation. In this study, minocycline was incorporated into the chitosan membrane by passive absorption at 5 or 10 mg/mL. The minocycline-loaded membranes and control membranes (carrier only) were tested against Porphyromonas gingivalis (P. gingivalis) by repeated zone of inhibition (ZOI) measurements. Testing showed that uncrosslinked and genipin-crosslinked membranes have similar capacity to absorb aqueous solutions (swelling ratio 1.7 -2.2). Minocycline loading resulted in bacterial inhibition for up to 8 days from crosslinked membranes (with 11 mm initial ZOI) whereas uncrosslinked membranes loaded with minocycline only inhibited bacteria for 4 days (with 8 mm initial ZOI). These in vitro results suggest that genipin-crosslinked electrospun chitosan membranes loaded with minocycline may be able to reduce early bacterial contamination of GTR graft sites.

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Section: Introductionmentioning

confidence: 99%

Antimicrobial Activity of Minocycline-Loaded Genipin-Crosslinked Nano-Fibrous Chitosan Mats for Guided Tissue Regeneration

Norowski¹,

Babu²,

Adatrow³

et al. 2012

JBNB

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“…Chitosancoated culture plates have been reported to upregulate genes associated with Coll-1, osteopontin, osteonectin, and OC in osteoblasts (38). Similarly, it has been shown that chitosan-coated fibers increased cell attachment, ALP activity, and the expression of osteopontin on preosteoblasts, resulting in significant mineralization in a 3D construct (39) and new bone formation in a rabbit calvarial model (40). Our in vivo experiment differs from the in vitro results from Matthews et al (38) and Yang et al (39) as the expression of Coll-1 was not modified, the specific ALP activity was reduced, and a trend toward reduction in the expression of OC was found.…”

Section: Discussionmentioning

confidence: 96%

Suture materials affect peri-implant bone healing and implant osseointegration

et al. 2015

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The aim of this study was to evaluate the effects of the remnants of two suture materials on osseointegration of titanium implants in a rabbit tibial model. Calibrated defects were prepared in the tibia of five Chinchilla rabbits. Filaments of nonresorbable (NR) nylon or resorbable (R) chitosan were placed at the bone to implant interface, whereas control sites had no suture material. After a healing period of 4 weeks, a pull-out test procedure was performed followed by enzymatic analyses of the wound fluid and relative quantification of mRNA levels for bone-related and cytokine markers from the peri-implant bone. A trend toward a reduced pull-out force was observed in the NR group (NR: 23.0 ± 12.8 N; R: 33.9 ± 11.3 N; control: 33.6 ± 24.0 N). Similarly, the bone resorption marker vacuolar type H+-ATPase was increased in the NR group compared with that in the control group (P = 0.041). The R group showed trends for lower alkaline phosphatase activity and osteocalcin expression and higher total protein content and RNA compared with the control group. In this submerged healing model, peri-implant bone healing was marginally affected by the two suture materials tested. However, there was a tendency toward better osseointegration and lower expression of bone resorption markers in the R group compared with the control group. (J Oral Sci 57, 219-227, 2015)Keywords: suture materials; chitosan; nylon; dental implants; bone; wound healing; osseointegration; biological complications. IntroductionForeign materials, such as cement remnants from the supraconstruction, can sometimes lead to peri-implant tissue complications (1). Theoretically, suture remnants displaced toward the peri-implant bone may have a similar negative effect on the peri-implant tissues. The choice of suture material may potentially influence the outcome of periodontal and implant procedures (2,3). The ideal suture material should provide uncomplicated stable primary wound closure without affecting the treatment outcome negatively. However, suture materials often seem to worsen the inflammatory response for a variety of reasons (4); therefore, the selection of an appropriate material is important (5,6). Materials that minimize inflammatory reactions are preferred, particularly in an environment, such as the oral cavity, where microbial levels are high. Moreover, suture materials and their metabolites might affect the healing process, particularly after implant placement, and can also provide a route for communication between the fixture and the oral cavity (2). Moreover, the wicking effect of some suture types can result in harbouring and transporting bacteria to the depth of the wound; thus, evading the host defense mechanism, resulting in local infection and subsequent

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“…An ideal material for bone regeneration should be biocompatible, biodegradable, easy to apply, and effective on bone repair. Since chitosan has been shown by many researchers to possess these properties 14,15) , this study was performed to evaluate its effectiveness on the healing of extraction socket.…”

Section: Discussionmentioning

confidence: 99%

Effect of Chitosan on Expression of Osteogenic Genes during the Healing of Rat Extraction Socket

Youn¹,

Jung²,

Lee³

et al. 2014

Journal of Korean Dental Science

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Biological Evaluation of Chitosan Nanofiber Membrane for Guided Bone Regeneration

Abstract: The biocompatibility of the chitosan nanofiber membrane was confirmed, with enhanced bone regeneration and no evidence of an inflammatory reaction. This experiment shows that the novel biodegradable chitosan nanofiber membrane may be useful as a tool for guided bone regeneration.

Cited by 160 publications

References 32 publications

Antimicrobial Activity of Minocycline-Loaded Genipin-Crosslinked Nano-Fibrous Chitosan Mats for Guided Tissue Regeneration

Antimicrobial Activity of Minocycline-Loaded Genipin-Crosslinked Nano-Fibrous Chitosan Mats for Guided Tissue Regeneration

Suture materials affect peri-implant bone healing and implant osseointegration

Effect of Chitosan on Expression of Osteogenic Genes during the Healing of Rat Extraction Socket

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