Biological evaluation and molecular dynamics simulation of water-soluble fullerene derivative C60[C(COOH)2]3

Gaponenko, Ivan N.; Ageev, Sergei V.; Iurev, Gleb O.; Shemchuk, Olga S.; Meshcheriakov, Anatolii A.; Petrov, Andrey V.; Solovtsova, Irina L.; Vasina, Lubov V.; Tennikova, Tatiana; Murin, I. V.; Semenov, Konstantin N.; Sharoyko, Vladimir V.

doi:10.1016/j.tiv.2019.104683

Cited by 27 publications

(4 citation statements)

References 54 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The genotoxicity of C 60 (OH) 24 was evaluated using the method of DNA comets based on measuring the effect of C 60 (OH) 24 on the integrity of the DNA of human peripheral blood mononuclear cells (PBMC) using alkaline gel electrophoresis . The DNA comets were stained with the propidium iodide aqueous solution ( C = 10 μg·mL –1 ) and visualized using a Micromed 3 LUM fluorescence microscope (Russia).…”

Section: Methodsmentioning

confidence: 99%

In Vitro and In Silico Investigation of Water-Soluble Fullerenol C₆₀(OH)₂₄: Bioactivity and Biocompatibility

et al. 2021

Self Cite

View full text Add to dashboard Cite

Light fullerenes, C 60 and C 70 , have significant potential in biomedical applications due to their ability to absorb reactive oxygen species, inhibit the development of tumors, inactivate viruses and bacteria, and as the basis for developing systems for targeted drug delivery. However, the hydrophobicity of individual fullerenes complicates their practical use; therefore, creating water-soluble derivatives of fullerenes is increasingly important. Currently, the most studied soluble adducts of fullerenes are polyhydroxy fullerenes or fullerenols. Unfortunately, investigations of fullerenol biocompatibility are fragmental. They often lack reproducibility both in the synthesis of the compounds and their biological action. We here investigate the biocompatibility of a welldefined fullerenol C 60 (OH) 24 obtained using methods that minimize the content of impurities and quantitatively characterize the product's composition. We carry out comprehensive biochemical and biophysical investigations of C 60 (OH) 24 that include photodynamic properties, cyto-and genotoxicity, hemocompatibility (spontaneous and photo-induced hemolysis, platelet aggregation), and the thermodynamic characteristics of C 60 (OH) 24 binding to human serum albumin and DNA. The performed studies show good biocompatibility of fullerenol C 60 (OH) 24 , which makes it a promising object for potential use in biomedicine.

show abstract

Section: Methodsmentioning

confidence: 99%

In Vitro and In Silico Investigation of Water-Soluble Fullerenol C₆₀(OH)₂₄: Bioactivity and Biocompatibility

et al. 2021

Self Cite

View full text Add to dashboard Cite

show abstract

“…The cytotoxicity of atranorin was studied using the MTT method on the cell lines Human Umbilical Vein Endothelial Cells (ECV340) and Human Embryonic Kidney 293 (HEK293) 24,29,31,35 .…”

Section: Study Of Biocompatibility and Biological Activitymentioning

confidence: 99%

“…Concentrations of atranorin varied in the range of 10-100 μM; samples were incubated for 15 h at 37 °C and analysed by electrophoresis in 1 % agarose gel. Also, the genotoxicity of atranorin was assessed using the DNA comet assay, based on measuring the effect of substances under study on the DNA integrity of human PBMCs using alkaline microgel electrophoresis 35 . DNA comets were visualised using a Micromed 3 LUM fluorescence microscope (Russia).…”

Section: Study Of Biocompatibility and Biological Activitymentioning

confidence: 99%

Atranorin is a novel potential candidate drug for treating myelodysplastic syndrome

Semenov,

Prokopiev,

Petukhova

et al. 2024

Preprint

Self Cite

View full text Add to dashboard Cite

This work is devoted to the study of biocompatibility, cyto- and genotoxicity, mechanism of action and prospects for the use of atranorin, which is an AKT kinase inhibitor, for the treatment of myelodysplastic syndrome. Atranorin was isolated by preparative flash chromatography; identification was carried out by UV, IR, and NMR spectroscopy, mass spectrometry, and elemental analysis. Biocompatibility studies included studies of haemocompatibility, genotoxicity, antioxidant activity, cytotoxicity against ECV340 and HEK293 cell lines. Computer modelling of the interaction of atranorin with AKT kinase was carried out using docking followed by molecular dynamics of the resulting complexes; the ADMET properties of atranorin were also calculated. Flow cytometry included analysis of the expression level of PD-L1 and TIM-3 in the presence of atranorin on THP-1, Mono-Mac-1 and KG-1 cell lines, as well as human bone marrow cells.

show abstract

“…The results of another study (10)of DNA damage in human lymphocytes using the DNA comet method showed that the compound C60[C(COOH)2]3 is not genotoxic to Homo sapiens lymphocytes.…”

Section: Introductionmentioning

confidence: 99%

Solubilizer of Bacterial Origin Surfactin Increases the Biological Activity of C60 Fullerene

Emelyantsev,

Prazdnova,

Chistyakov

2023

Preprint

View full text Add to dashboard Cite

Currently, there exists conflicting data regarding the biological activity of unmodified fullerene C60. Various sources report its toxicity, geroprotective activity, and potential interaction with DNA. Contradictory findings regarding the toxicity of C60may arise from the use of toxic solvents, as well as the influence of bioavailability and bioactivity on the preparation conditions of C60suspensions. Furthermore, the microbiota of experimental animals can impact geroprotective activity results by releasing surfactants that facilitate substance penetration through the cell membrane.In this study, we selected conditions for solubilizing fullerene C60in a solution of surfactin, a surfactant of bacterial origin, as well as in a 2% aqueous solution of TWEEN 80, employing ultrasound. Through bioluminescent analysis using lux biosensors in E. coli MG1655, we observed that C60in surfactin reduced induced genotoxic and oxidative stress. Given that surfactin enhances membrane permeability to fullerene C60, suspensions of fullerene in designated concentrations of surfactin can be regarded as a DNA protector and antioxidant, warranting further investigation as a promising component of novel drugs.

show abstract

Biological evaluation and molecular dynamics simulation of water-soluble fullerene derivative C60[C(COOH)2]3

Cited by 27 publications

References 54 publications

In Vitro and In Silico Investigation of Water-Soluble Fullerenol C₆₀(OH)₂₄: Bioactivity and Biocompatibility

In Vitro and In Silico Investigation of Water-Soluble Fullerenol C₆₀(OH)₂₄: Bioactivity and Biocompatibility

Atranorin is a novel potential candidate drug for treating myelodysplastic syndrome

Solubilizer of Bacterial Origin Surfactin Increases the Biological Activity of C60 Fullerene

Contact Info

Product

Resources

About

Biological evaluation and molecular dynamics simulation of water-soluble fullerene derivative C60[C(COOH)2]3

Cited by 27 publications

References 54 publications

In Vitro and In Silico Investigation of Water-Soluble Fullerenol C60(OH)24: Bioactivity and Biocompatibility

In Vitro and In Silico Investigation of Water-Soluble Fullerenol C60(OH)24: Bioactivity and Biocompatibility

Atranorin is a novel potential candidate drug for treating myelodysplastic syndrome

Solubilizer of Bacterial Origin Surfactin Increases the Biological Activity of C60 Fullerene

Contact Info

Product

Resources

About

In Vitro and In Silico Investigation of Water-Soluble Fullerenol C₆₀(OH)₂₄: Bioactivity and Biocompatibility

In Vitro and In Silico Investigation of Water-Soluble Fullerenol C₆₀(OH)₂₄: Bioactivity and Biocompatibility