Biological efficacy of silk fibroin nanofiber membranes for guided bone regeneration

Kim, Kyoung-Hwa; Jeong, Lim; Park, Ho-Nam; Shin, Seung‐Yun; Park, Won Ho; Lee, Sangchul; Kim, Tae Il; Park, Yoon-Jeong; Seol, Yang‐Jo; Lee, Yong-Moo; Ku, Young; Rhyu, In‐Chul; Han, Soo-Boo; Chung, Chong-Pyoung

doi:10.1016/j.jbiotec.2005.06.033

Cited by 310 publications

(163 citation statements)

References 33 publications

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“…Cai et al (13) have reported that a poly (D,L-lactic acid) surface modified with 30 kDa of silk fibroin showed more ALP activity than an untreated poly (D,L-lactic acid) surface. In addition, MC3T3-E1 cells cultured on a silk fibroin membrane were reported to show ALP activity levels comparable to those on a surfacetreated culture dish (14). Other researchers have demonstrated that when silk fibroin is cultured with the bone cells or stem cells, bone tissue growth in vitro is induced (9,10).…”

Section: Resultsmentioning

confidence: 96%

Low molecular weight silk fibroin increases alkaline phosphatase and type I collagen expression in MG63 cells

Kim¹,

Choi²,

Jeong³

et al. 2010

BMB Reports

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Section: Resultsmentioning

confidence: 96%

Low molecular weight silk fibroin increases alkaline phosphatase and type I collagen expression in MG63 cells

Kim¹,

Choi²,

Jeong³

et al. 2010

BMB Reports

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“…An ideal periosteal graft would not only provide a physical structure that facilitates osteoconduction, but also osteoinductive signals that stimulate osteogenesis and ultimately promote biomineralization [8]. Membranes made of amniotic tissue [9], chitosan-silica [10] or silk fibroin nanofibers [11] have been reported to induce osteoblastic differentiation in vitro. Also, a variety of in vitro mineralizing membranes based on chitosan/bioactive glass nanoparticles [12], platelet-rich fibrin functionalized with alkaline phosphatase (ALK) [13], polycaprolactone fibers incorporating nano-apatite particles [14] or forsterite nanopowder [15], and collagen [16] have been developed.…”

Section: Introductionmentioning

confidence: 99%

Mineralization and bone regeneration using a bioactive elastin-like recombinamer membrane

et al. 2014

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“…Furthermore, some studies have certified that electrospun fibers alone can be tailored to fabricate functional scaffolds in bone tissue engineering and enhance bone regeneration to a certain extent without any inflammatory reaction. 11,20 During recent decades, many electrospun polymers have been used for bone tissue engineering, which include both nonbiodegradable and biodegradable polymers either with a synthetic or natural origin. Among them, nonbiodegradable electrospun polymers such as polyurethane and polyester urethane could interfere with tissue turnover and remodeling due to their slow degradation, even though they possessed substantial mechanical stability.…”

Section: Introductionmentioning

confidence: 99%

Preparation of poly(ethylene glycol)/polylactide hybrid fibrous scaffolds for bone tissue engineering

Fu²,

Fan³

et al. 2011

IJN

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Polylactide (PLA) electrospun fibers have been reported as a scaffold for bone tissue engineering application, however, the great hydrophobicity limits its broad application. In this study, the hybrid amphiphilic poly(ethylene glycol) (PEG)/hydrophobic PLA fibrous scaffolds exhibited improved morphology with regular and continuous fibers compared to corresponding blank PLA fiber mats. The prepared PEG/PLA fibrous scaffolds favored mesenchymal stem cell (MSC) attachment and proliferation by providing an interconnected porous extracellular environment. Meanwhile, MSCs can penetrate into the fibrous scaffold through the interstitial pores and integrate well with the surrounding fibers, which is very important for favorable application in tissue engineering. More importantly, the electrospun hybrid PEG/PLA fibrous scaffolds can enhance MSCs to differentiate into bone-associated cells by comprehensively evaluating the representative markers of the osteogenic procedure with messenger ribonucleic acid quantitation and protein analysis. MSCs on the PEG/PLA fibrous scaffolds presented better differentiation potential with higher messenger ribonucleic acid expression of the earliest osteogenic marker Cbfa-1 and mid-stage osteogenic marker Col I . The significantly higher alkaline phosphatase activity of the PEG/PLA fibrous scaffolds indicated that these can enhance the differentiation of MSCs into osteoblast-like cells. Furthermore, the higher messenger ribonucleic acid level of the late osteogenic differentiation markers OCN ( osteocalcin ) and OPN ( osteopontin ), accompanied by the positive Alizarin red S staining, showed better maturation of osteogenic induction on the PEG/PLA fibrous scaffolds at the mineralization stage of differentiation. After transplantation into the thigh muscle pouches of rats, and evaluating the inflammatory cells surrounding the scaffolds and the physiological characteristics of the surrounding tissues, the PEG/PLA scaffolds presented good biocompatibility. Based on the good cellular response and excellent osteogenic potential in vitro, as well as the biocompatibility with the surrounding tissues in vivo, the electrospun PEG/PLA fibrous scaffolds could be one of the most promising candidates in bone tissue engineering.

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Biological efficacy of silk fibroin nanofiber membranes for guided bone regeneration

Cited by 310 publications

References 33 publications

Low molecular weight silk fibroin increases alkaline phosphatase and type I collagen expression in MG63 cells

Low molecular weight silk fibroin increases alkaline phosphatase and type I collagen expression in MG63 cells

Mineralization and bone regeneration using a bioactive elastin-like recombinamer membrane

Preparation of poly(ethylene glycol)/polylactide hybrid fibrous scaffolds for bone tissue engineering

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