Biological effects of the superoxide radical

Fridovich, Irwin

doi:10.1016/0003-9861(86)90526-6

Cited by 1,295 publications

(548 citation statements)

References 124 publications

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“…Antioxidant enzymes play a vital role in conferring tolerance to drought and other abiotic stresses. ROS such as superactive radicals, Hyderogen peroxide and the hydroxyl radicals liberated during stress can cause lipid peroxidation resulting in membrane damage, protein degradation (Davies 1987) and enzyme inactivation (Fridovich 1986). Plants have endogenous mechanism to protect cellular and sub-cellular system from toxic effects of these radicals in the form of antioxidant enzymes such as CAT, APX, POX and SOD (Larson 1988).…”

Section: Activity Of Antioxidant Enzymesmentioning

confidence: 99%

In vitro selection and characterization of polyethylene glycol (PEG) tolerant callus lines and regeneration of plantlets from the selected callus lines in sugarcane (Saccharum officinarum L.)

Srinath

Ftz

2013

Physiol Mol Biol Plants

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A system for in vitro selection of drought tolerant callus lines in sugarcane was developed. High molecular weight PEG was used as selective agent. Selected callus line grew better than non-selected callus when grown on different concentrations of PEG. The activity of antioxidant enzymes like CAT, POX, APX and SOD were high in selected callus than in non-selected callus. Osmolytes like proline and ascorbic acid were at higher levels in selected callus than in nonselected callus, however at higher concentrations (20-30 %) of PEG, levels of proline and ascorbic acid decreased. The frequency of organogenesis and number of plantlets decreased in selected callus than in non-selected callus. The results can be used for in vitro screening and manipulations of sugarcane for improvement of drought tolerance

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Section: Activity Of Antioxidant Enzymesmentioning

confidence: 99%

In vitro selection and characterization of polyethylene glycol (PEG) tolerant callus lines and regeneration of plantlets from the selected callus lines in sugarcane (Saccharum officinarum L.)

Srinath

Ftz

2013

Physiol Mol Biol Plants

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“…The pH of the various environments in in vivo and in vitro experimental systems is thus of great significance in determining the lifetime and diffusion path in solution of generated radicals, and their potential for direct reaction with cellular constituents. The reactivity of H0 2 /0 2~ in biological tissues has been extensively reviewed [25,34,54,55,64,93] and will not be dealt with here.…”

Section: The Chemistry Of Oxygen Radicals In Cellsmentioning

confidence: 99%

The generation of oxygen radicals during host plant responses to infection

Sutherland

1991

Physiological and Molecular Plant Pathology

323

168

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“…While Kupffer cell-derived radicals (e.g., superoxide anion) can either react directly with the surrounding lipid membrane (at low pHs [31]), or be converted to a hydroxyl radical that will react with lipids; in hepatocytes, excess peroxisomal H 2 O 2 conversion to a reactive hydroxyl radical in the presence of iron is the presumed mechanism of formation of lipid peroxides [32]. It should be noted that the utility of spin trapping with POBN for direct detection of hydroxyl radical, superoxide or nitric oxide is limited [33]; thus, the results of this study should be interpreted with caution.…”

Section: Direct Evidence For Peroxisome Proliferator-induced Sustainementioning

confidence: 99%

Sustained formation of α-(4-pyridyl-1-oxide)-N-tert-butylnitrone radical adducts in mouse liver by peroxisome proliferators is dependent upon peroxisome proliferator-activated receptor-α, but not NADPH oxidase

Woods

Burns

Maki

et al. 2007

Free Radical Biology and Medicine

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Reactive oxygen species are thought to be crucial for peroxisome proliferator-induced liver carcinogenesis. Free radicals have been shown to mediate the production of mitogenic cytokines by Kupffer cells and cause DNA damage in rodent liver. Previous in vivo experiments demonstrated that acute administration of the peroxisome proliferator di-(2-ethylhexyl) phthalate (DEHP) led to an increase in production of α-(4-pyridyl-1-oxide)-N-tert-butylnitrone (POBN) radical adducts in liver, an event that was dependent on Kupffer cell NADPH oxidase, but not peroxisome proliferator activated receptor (PPAR)α. Here, we hypothesized that continuous treatment with peroxisome proliferators will cause a sustained formation in POBN radical adducts in liver. Mice were fed diets containing either 4-chloro-6-(2,3-xylidino)-2-pyrimidinylthio acetic acid (WY-14,643, 0.05% w/w), or DEHP (0.6% w/w) for up to three weeks. Liver-derived radical production was assessed in bile samples by measuring POBN-radical adducts using electron spin resonance. Our data indicate that WY-14,643 causes a sustained increase in POBN radical adducts in mouse liver and that this effect is greater than that of DEHP. To understand the molecular source of these radical species, NADPH oxidase-deficient (p47 phox -null) and PPARα-null mice were examined after treatment with WY-14,643. No increase in radicals was observed in PPARα-null mice that were treated with WY-14,643 for 3 weeks, while the response in p47 phox -nulls was similar to that of wild-type mice. These results show that PPARα, not NADPH oxidase, is critical for a sustained increase in POBN radical production caused by peroxisome proliferators in rodent liver. Therefore, peroxisome proliferator-induced POBN radical production in Kupffer cells may be limited to an acute response to these compounds in mouse liver.

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Biological effects of the superoxide radical

Cited by 1,295 publications

References 124 publications

In vitro selection and characterization of polyethylene glycol (PEG) tolerant callus lines and regeneration of plantlets from the selected callus lines in sugarcane (Saccharum officinarum L.)

In vitro selection and characterization of polyethylene glycol (PEG) tolerant callus lines and regeneration of plantlets from the selected callus lines in sugarcane (Saccharum officinarum L.)

The generation of oxygen radicals during host plant responses to infection

Sustained formation of α-(4-pyridyl-1-oxide)-N-tert-butylnitrone radical adducts in mouse liver by peroxisome proliferators is dependent upon peroxisome proliferator-activated receptor-α, but not NADPH oxidase

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