Biological effects of multimetal (Ni, Cd, Pb, Cu, Cr, Zn) mixture in rainbow trout Oncorhynchus mykiss: Laboratory exposure and recovery study

Stankevičiūtė, Milda; Makaras, Tomas; Pažusienė, Janina; Čapukoitienė, Brigita; Sauliutė, Gintarė; Jurgelėnė, Živilė; Raudonytė-Svirbutavičienė, Eva; Jokšas, Kęstutis

doi:10.1016/j.ecoenv.2021.112202

Cited by 5 publications

(2 citation statements)

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“…Aquatic toxicology research enables the estimation of the negative effects of biological, chemical, or physical agents on aquatic organisms and ecosystems and establish the basis of necessary conservation policies (Nikinmaa, 2014). In recent years, detailed evaluation using the comet assay, micronucleus test, nuclear abnormalities of the genotoxic effects of metalloid and heavy metals for different groups such as drinking water, fish, and other organisms has further increased the popularity of these toxicological studies (Doğanlar et al, 2016; Gökalp et al, 2020; Kontaş and Bostancı, 2020; Stankevičiūtė et al, 2021). In addition, the effects of metal(oid)s on aquatic organisms, ecosystems, and human health need to be investigated using genotoxicity tests (Lah et al, 2005; Gökalp et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

“…There is no detailed study on the effects of the mixtures formed by the metal(oid)s on these regulation limits on fish. It has been reported that aquatic systems have long been exposed to metal(oid) (Al, As, and Mn) contamination, especially of industrial, agricultural, and floodplain origin (Kontaş and Bostancı, 2020; Stankevičiūtė et al, 2021; Sarkar et al, 2022). In this study, it was investigated whether low and high-dose mixtures of Al, As, and Mn metal(oid)s have potential genotoxic effects on Cyprinus carpio .…”

Section: Introductionmentioning

confidence: 99%

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In vivo assessment of genotoxic effects in Cyprinus carpio L., 1758 (Teleostei: Cyprinidae) exposed to selected metal(oid)s

Kontaş

2022

Toxicol Ind Health

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Heavy metals and metalloids originating from industrial, agricultural, and urban wastes and increasing in aquatic ecosystems cause genotoxic damage to fish species. This study aimed to determine the potential genotoxic effects of mixtures of aluminum, arsenic, and manganese in Cyprinus carpio. The effects of the mixtures on erythrocyte cells of C. carpio were examined using the comet assay, micronucleus test, and erythrocytic nuclear abnormalities in two groups after exposure to three doses of the mixtures (Group A; Dose 1: 0.3 + 0.1 + 0.02 mg/L, Dose 2: 0.6 + 0.2 + 0.04 mg/L, Dose 3: 0.9 + 0.3 + 0.06 mg/L and Group B; Dose 1: 1 + 3 +0.1 mg/L, Dose 2: 2 + 6 + 0.2 mg/L, Dose 3: 3 + 9 + 0.3 mg/L). Experimental groups were formed according to the permissible limits specified in the Turkish Surface Water Quality Regulation (TSWQR). The results of comet assay parameters such as tail DNA %, tail moment, and olive tail moment confirmed the genotoxic effect of metal(oid)s mixtures on erythrocyte cells compared with control groups and showed that DNA damage increased with increasing the concentrations. The micronucleus and other nuclear abnormalities such as blebbed nuclei, notched nuclei, eightshaped nuclei, lobed nuclei, and binucleated cells were detected in the erythrocyte cells exposed to the mixtures. Consequently, it was found that the frequency of micronucleus and erythrocytic nuclear abnormalities significantly increased in the erythrocyte cells exposed to metal(oid) concentrations compared to control groups. These results show the existence of potential genotoxicity in C. carpio even at the minimum values specified in the TSWQR after exposure to the mixtures.

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