Biological effects of milk proteins and their peptides with emphasis on those related to the gastrointestinal ecosystem

Baldi, Alfonso; Politis, I.; Pecorini, C.; Fusi, E.; Chronopoulou, Roubini; Dell’Orto, V.

doi:10.1017/s002202990500110x

Cited by 76 publications

(64 citation statements)

References 28 publications

(30 reference statements)

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“…These usually have a latent activity within the native protein and are generated after proteolysis during gastrointestinal transit (24), acquiring an active role.…”

Section: Mfg M Protein Fragmentsmentioning

confidence: 99%

Proteolysis of Milk Fat Globule Membrane Proteins in Preterm Milk: A Transient Phenomenon with a Possible Biological Role?

Giuffrida

Cavaletto

Lamberti

et al. 2008

Int J Immunopathol Pharmacol

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Milk fat globule membrane (MFGM) proteins constitute a milk fraction currently of great interest, as they appear to significantly contribute to milk protective role. We investigated these proteins in human preterm colostrum and milk. For the former we found a peculiar 2-DE pattern, with a spot concentration at low molecular weight, which mass spectrometry analysis showed to be fragments belonging to some MFGM proteins with a well-known biological and especially immunological role: lactadherin, membrane-associated lactoferrin, butyrophilin, clusterin and heavy-chain immunoglobulin. Since we were able to rule out protease activity after specimen collection, we hypothesize the localization of the proteolytic enzymes in the alveolar cell membranes of the mammary gland. This mechanism is probably under hormonal control and the unexpected advent of preterm delivery would not allow hormonal conditions typical of lactation to occur immediately, causing a delay in enzymatic inhibition. This hypothesis is supported by some of our results, picturing a peculiar transient phenomenon of adaptation of the mammary-gland-membrane proteins after preterm delivery. Further studies will be required to verify whether the presence of protein fragments exerts a specific biological and immuno-defensive role in preterm infants, thus adding evidence to the outstanding biological role and benefits of mother's own milk in feeding preterm infants.

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“…These usually have a latent activity within the native protein and are generated after proteolysis during gastrointestinal transit (24), acquiring an active role.…”

Section: Mfg M Protein Fragmentsmentioning

confidence: 99%

Proteolysis of Milk Fat Globule Membrane Proteins in Preterm Milk: A Transient Phenomenon with a Possible Biological Role?

Giuffrida

Cavaletto

Lamberti

et al. 2008

Int J Immunopathol Pharmacol

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“…1 Introduction the newborn, allowed through the passage of milk proteins and peptides, which, after digestion, stimulate the mucosa immune system of the infant (Baldi et al 2005). When the mother is not able to breast feed, or after weaning, it becomes important to find an adequate alternative nourishment.…”

mentioning

confidence: 99%

Whey proteins and their antimicrobial properties in donkey milk: a brief review

Brumini

Criscione

Bordonaro

et al. 2015

Dairy Sci. & Technol.

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Milk is a source of bioactive compounds essential for health and growth of newborns. Donkey milk, rich in lactose and whey proteins, has been proven to be a good breast milk substitute during infancy and adequate nourishment for patients with cow milk protein allergy. Beside, this milk is gaining a growing interest for human nutrition because of some other alleged health benefit. It shows antibacterial activity toward a wide range of Gram-positive and Gram-negative bacteria, stimulates immune system in convalescence, regulates gastrointestinal flora, and prevents inflammatory and autoimmune diseases. As regards its antimicrobial properties, although all the milk components might contribute to this activity, the whey protein fraction of donkey milk is generally believed to play the main role. The aim of this review is to highlight the antimicrobial properties of donkey milk with a special focus on the whey protein fraction. The effects of preservation and processing treatments on whey protein content and antimicrobial activity are also discussed.

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“…In recent years, the bioactive peptides derived from milk proteins were found to have anticancer function, especially for gynecological cancer (5)(6)(7). Although these bioactive peptides were not as effective as chemotherapy drugs such as DDP in the treatment of cancer, they have no toxicity, or low toxicity to normal cells (untransformed cells).…”

Section: Introductionmentioning

confidence: 99%

The milk-derived hexapeptide PGPIPN inhibits the invasion and migration of human ovarian cancer cells by regulating the expression of MTA1 and NM23H1 genes

Zhao

Cai

Yang

et al. 2016

International Journal of Oncology

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Abstract. Some bioactive peptides derived from natural resources or synthesized by rational design have been proved to have very good anticancer effect. We studied the inhibition of PGPIPN, a hexapeptide derived from bovine β-casein, on the invasion and metastasis of human ovarian cancer cells in vitro and its molecular mechanism. The human ovarian cancer cells studied include the cell line SKOV 3 as well as the primary ovarian cancer cells from ovarian tumor tissues of 37 patients at initial debulking surgery, diagnosed as serous ovarian adenocarcinoma. We showed that PGPIPN inhibited the invasion of ovarian cancer cells with Transwell chamber assay, the migration of ovarian cancer cells with cell scratch assay and colony formation of ovarian cancer cells. The expression (mRNAs and proteins) of genes relevant to invasion and metastasis, MTA1, and NM23H1 were analyzed by real-time PCR and western blotting. PGPIPN repressed the expression of MTA1, and promoted NM23H1. The effects of PGPIPN were dose-dependent. Thus, our study suggests that PGPIPN is a potential therapeutic agent for adjuvant therapy of human malignant ovarian tumors.

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Biological effects of milk proteins and their peptides with emphasis on those related to the gastrointestinal ecosystem

Cited by 76 publications

References 28 publications

Proteolysis of Milk Fat Globule Membrane Proteins in Preterm Milk: A Transient Phenomenon with a Possible Biological Role?

Proteolysis of Milk Fat Globule Membrane Proteins in Preterm Milk: A Transient Phenomenon with a Possible Biological Role?

Whey proteins and their antimicrobial properties in donkey milk: a brief review

The milk-derived hexapeptide PGPIPN inhibits the invasion and migration of human ovarian cancer cells by regulating the expression of MTA1 and NM23H1 genes

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