Biological Effects of Gyrophoric Acid and Other Lichen Derived Metabolites, on Cell Proliferation, Apoptosis and Cell Signaling pathways

Mohammadi, Mahshid; Bagheri, Leila; Badreldin, Amr A.; Fatehi, Pedram; Pakzad, Leila; Suntres, Zacharias E.; Wijnen, André J. van

doi:10.1016/j.cbi.2021.109768

Cited by 22 publications

(19 citation statements)

References 80 publications

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“…In accordance to our hypothesis, several studies revealed that the genus Pseudomonas was dominant in PAH-contaminated sites and that hydrocarbon degradation ability and antibiotic resistance were strongly correlated in these bacteria [84]. In addition, the emergence of AMR genes was demonstrated to be 15 times higher in contaminated sites [85] [86].…”

Section: Discussionsupporting

confidence: 87%

Rhizocarpon geographicumlichen discloses a highly diversified microbiota carrying antibiotic resistance and Persistent Organic Pollutants tolerance

Miral

Kautsky

Alves-Carvalho

et al. 2022

Preprint

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As rock-inhabitants, lichens are exposed to extreme and fluctuating abiotic conditions associated with poor sources of nutriments. These extreme conditions confer to lichens the unique ability to develop protective mechanisms. Consequently, lichen-associated microbes disclose highly versatile lifestyles and ecological plasticity, enabling them to withstand extreme environments. Because of their ability to grow on poor and extreme habitats, bacteria associated with lichens can tolerate a wide range of pollutants and on the other hand secrete antimicrobial compounds. In addition, lichen-associated bacteria were described to harbor ecological functions crucial for the evolution of the lichen holobiont. Nevertheless, the ecological features of lichen-associated microbes are still underestimated. To explore the untapped ecological diversity of lichen-associated bacteria, we adopted a novel culturomic approach on the crustose lichen Rhizocarpon geographicum. We sampled R. geographicum in French habitats exposed to oil spill and we combined 9 culturing methods with 16S rRNA sequencing to capture the largest bacterial diversity. A deep functional analysis of the lichen-associated bacterial collection showed the presence of a set of bacterial strains resistant to a wide range of antibiotics and displaying tolerance to Persistent Organic Pollutants (POP). Our study is a starting point to explore the ecological features of the lichen microbiota.

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Section: Discussionsupporting

confidence: 87%

Rhizocarpon geographicumlichen discloses a highly diversified microbiota carrying antibiotic resistance and Persistent Organic Pollutants tolerance

Miral

Kautsky

Alves-Carvalho

et al. 2022

Preprint

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“…Lichen secondary metabolites hold great promise for biopharmaceutical applications [ 1 ]. However, data regarding their potential use as anti-GBM agents are scarce.…”

Section: Discussionmentioning

confidence: 99%

“…Lichens are a source of ~1000 unique secondary metabolites of various chemical structures, including aliphatic, cycloaliphatic, aromatic, and terpenic compounds [ 1 , 2 ]. These substances are usually deposited on the surface of mycelium cells and are mostly water-insoluble [ 3 ].…”

Section: Introductionmentioning

confidence: 99%

Lichen Secondary Metabolites Inhibit the Wnt/β-Catenin Pathway in Glioblastoma Cells and Improve the Anticancer Effects of Temozolomide

Majchrzak‐Celińska

Kleszcz

Studzińska-Sroka

et al. 2022

Cells

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Lichens are a source of secondary metabolites with significant pharmacological potential. Data regarding their possible application in glioblastoma (GBM) treatment are, however, scarce. The study aimed at analyzing the mechanism of action of six lichen secondary metabolites: atranorin, caperatic acid, physodic acid, squamatic acid, salazinic acid, and lecanoric acid using two- and three-dimensional GBM cell line models. The parallel artificial membrane permeation assay was used to predict the blood-brain barrier penetration ability of the tested compounds. Their cytotoxicity was analyzed using the MTT test on A-172, T98G, and U-138 MG cells. Flow cytometry was applied to the analysis of oxidative stress, cell cycle distribution, and apoptosis, whereas qPCR and microarrays detected the induced transcriptomic changes. Our data confirm the ability of lichen secondary metabolites to cross the blood-brain barrier and exert cytotoxicity against GBM cells. Moreover, the compounds generated oxidative stress, interfered with the cell cycle, and induced apoptosis in T98G cells. They also inhibited the Wnt/β-catenin pathway, and this effect was even stronger in case of a co-treatment with temozolomide. Transcriptomic changes in cancer related genes induced by caperatic acid and temozolomide were the most pronounced. Lichen secondary metabolites, caperatic acid in particular, should be further analyzed as potential anti-GBM agents.

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“…Based on the chemical structure of lichen compounds and its potential antioxidant activity, the depsides gyrophoric acid and lecanoric acid have carboxyl and hydroxyl groups that interact with several enzymatic active sites. Moreover, the aromatic rings of gyrophoric acid and lecanoric acid are responsible for their free radical scavenging properties [ 38 , 39 ]. Among the lichens investigated in this study, D. artica was the most active specie.…”

Section: Discussionmentioning

confidence: 99%

Lichen Extracts from Cetrarioid Clade Provide Neuroprotection against Hydrogen Peroxide-Induced Oxidative Stress

Hernández-Martín

González-Burgos²,

Divakar³

et al. 2022

Molecules

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Oxidative stress is involved in the pathophysiology of many neurodegenerative diseases. Lichens have antioxidant properties attributed to their own secondary metabolites with phenol groups. Very few studies delve into the protective capacity of lichens based on their antioxidant properties and their action mechanism. The present study evaluates the neuroprotective role of Dactylina arctica, Nephromopsis stracheyi, Tuckermannopsis americana and Vulpicida pinastri methanol extracts in a hydrogen peroxide (H2O2) oxidative stress model in neuroblastoma cell line “SH-SY5Y cells”. Cells were pretreated with different concentrations of lichen extracts (24 h) before H2O2 (250 µM, 1 h). Our results showed that D. arctica (10 µg/mL), N. stracheyi (25 µg/mL), T. americana (50 µg/mL) and V. pinastri (5 µg/mL) prevented cell death and morphological changes. Moreover, these lichens significantly inhibited reactive oxygen species (ROS) production and lipid peroxidation and increased superoxide dismutase (SOD) and catalase (CAT) activities and glutathione (GSH) levels. Furthermore, they attenuated mitochondrial membrane potential decline and calcium homeostasis disruption. Finally, high-performance liquid chromatography (HPLC) analysis revealed that the secondary metabolites were gyrophoric acid and lecanoric acid in D. artica, usnic acid, pinastric acid and vulpinic acid in V. pinastri, and alectoronic acid in T. americana. In conclusion, D. arctica and V. pinastri are the most promising lichens to prevent and to treat oxidative stress-related neurodegenerative diseases.

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Biological Effects of Gyrophoric Acid and Other Lichen Derived Metabolites, on Cell Proliferation, Apoptosis and Cell Signaling pathways

Cited by 22 publications

References 80 publications

Rhizocarpon geographicumlichen discloses a highly diversified microbiota carrying antibiotic resistance and Persistent Organic Pollutants tolerance

Rhizocarpon geographicumlichen discloses a highly diversified microbiota carrying antibiotic resistance and Persistent Organic Pollutants tolerance

Lichen Secondary Metabolites Inhibit the Wnt/β-Catenin Pathway in Glioblastoma Cells and Improve the Anticancer Effects of Temozolomide

Lichen Extracts from Cetrarioid Clade Provide Neuroprotection against Hydrogen Peroxide-Induced Oxidative Stress

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